FC876330

Overview
NameFC876330
Unique NameFC876330
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length634
Libraries
Library NameType
DevOvary1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig160 contig Ccv1_Contig160:13..647. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 8.851e-16
Identity = 32/92 (34.78%), Postives = 43/92 (46.74%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVGFWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+     RN       GF+ Y +FI A   +   GFGT+G    + +EI AF A    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   89 FDQMLKHRNDNACQGKGFYSYNAFINAARSFP--GFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPS 178

HSP 2 Score: 39.2762 bits (90), Expect = 8.851e-16
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  181 WPCAPGRKYFGRGPIQISHNYNYGPCG 207
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 8.856e-16
Identity = 32/92 (34.78%), Postives = 43/92 (46.74%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVGFWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+     RN       GF+ Y +FI A   +   GFGT+G    + +EI AF A    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   84 FDQMLKHRNDNACQGKGFYSYNAFINAARSFP--GFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPS 173

HSP 2 Score: 39.2762 bits (90), Expect = 8.856e-16
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  176 WPCAPGRKYFGRGPIQISHNYNYGPCG 202
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI2_PEA (Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 1.944e-15
Identity = 33/102 (32.35%), Postives = 46/102 (45.10%), Query Frame = 2 
Query:  236 TISDYFQVYQFENFFSKRNTPVAHAVGFWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            T+S       F N    RN        F+ Y +F++A   +    F   G    K +EI AFL     +T+ G+  A  GP AWG C+  E +PS +YC  S
Sbjct:   67 TLSSLISGDTFNNMLKHRNDNACQGKPFYTYDAFLSAAKAFP--NFANKGDTATKKREIAAFLGQTSHETTGGWPTAPDGPYAWGYCFLREQNPS-TYCQAS 165

HSP 2 Score: 43.5134 bits (101), Expect = 1.944e-15
Identity = 16/27 (59.26%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC  G +YY RG I I WNYNYG  G
Sbjct:  168 FPCASGKQYYGRGPIQISWNYNYGQCG 194
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHIC_SOLLC (Basic 30 kDa endochitinase OS=Solanum lycopersicum GN=CHI9 PE=1 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 7.205e-15
Identity = 33/93 (35.48%), Postives = 45/93 (48.39%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVG-FWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+   + RN         F+ Y +F+TA   +   GFGTTG    + +EI AFLA    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   81 FDQMLNHRNDNACQGKNNFYSYNAFVTAAGSFP--GFGTTGDITARKREIAAFLAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPS 171

HSP 2 Score: 39.2762 bits (90), Expect = 7.205e-15
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  174 WPCAPGRKYFGRGPIQISHNYNYGPCG 200
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI2_SOLTU (Endochitinase 2 (Fragment) OS=Solanum tuberosum GN=CHTB2 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 9.367e-15
Identity = 33/93 (35.48%), Postives = 45/93 (48.39%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVG-FWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+   + RN       G F+ Y +FI+A   +   GFGTTG    + +EI AF A    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   75 FDQMLNHRNDNACQGKGNFYSYNAFISAAGSFP--GFGTTGDITARKREIAAFFAQTSHETTGGWASAPDGPYAWGYCFLREQGSPGDYCTPS 165

HSP 2 Score: 39.2762 bits (90), Expect = 9.367e-15
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  168 WPCAPGRKYFGRGPIQISHNYNYGPCG 194
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI1_SOLTU (Endochitinase 1 (Fragment) OS=Solanum tuberosum GN=CHTB1 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 9.367e-15
Identity = 33/93 (35.48%), Postives = 45/93 (48.39%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVG-FWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+   + RN       G F+ Y +FI+A   +   GFGTTG    + +EI AF A    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   77 FDQMLNHRNDNACQGKGNFYSYNAFISAAGSFP--GFGTTGDITARKREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPS 167

HSP 2 Score: 39.2762 bits (90), Expect = 9.367e-15
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  170 WPCAPGRKYFGRGPIQISHNYNYGPCG 196
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI9_ORYSJ (Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht9 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.051e-14
Identity = 29/89 (32.58%), Postives = 43/89 (48.31%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVGFWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYC 532
            FE     RN     A GF+ Y + +TA   +    F  TG    + +E+ AFL     +T+ G+  A  GP +WG C+  E+  + SYC
Sbjct:   95 FEQLLLHRNDAACPARGFYTYDALVTAAAAFPD--FAATGDDEARKREVAAFLGQTSHETTGGWATAPDGPYSWGYCFKEEIGATASYC 181

HSP 2 Score: 37.7354 bits (86), Expect = 2.051e-14
Identity = 13/27 (48.15%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC P  +Y+ RG I + +NYNYG  G
Sbjct:  187 WPCAPDKKYFGRGPIQLSYNYNYGPAG 213
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI3_SOLTU (Endochitinase 3 (Fragment) OS=Solanum tuberosum GN=CHTB3 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.670e-14
Identity = 33/93 (35.48%), Postives = 45/93 (48.39%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVG-FWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+   + RN         F+ Y +FI+A   +   GFGTTG    + +EI AFLA    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   77 FDQMLNHRNDNACQGKNNFYSYNAFISAAGSFP--GFGTTGDITARKREIAAFLAQTSHETTGGWPSAPDGPYAWGYCFLREQGSPGDYCTPS 167

HSP 2 Score: 39.2762 bits (90), Expect = 2.670e-14
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  170 WPCAPGRKYFGRGPIQISHNYNYGPCG 196
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 4.501e-14
Identity = 31/92 (33.70%), Postives = 42/92 (45.65%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVGFWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+     RN        F+ Y +FI A   +   GFGT+G    + +EI AF A    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   88 FDQMLKHRNENSCQGKNFYSYNAFINAARSFP--GFGTSGDINARKREIAAFFAQTSHETTGGWASAPDGPYAWGYCFLRERGNPGDYCPPS 177

HSP 2 Score: 39.2762 bits (90), Expect = 4.501e-14
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  180 WPCAPGRKYFGRGPIQISHNYNYGPCG 206
[Close]
BLAST of FC876330 vs. ExPASy Swiss-Prot
Match: CHI4_SOLTU (Endochitinase 4 (Fragment) OS=Solanum tuberosum GN=CHTB4 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 5.864e-14
Identity = 32/93 (34.41%), Postives = 42/93 (45.16%), Query Frame = 2 
Query:  266 FENFFSKRNTPVAHAVG-FWDYQSFITATVKYQPLGFGTTGTKLDKMKEICAFLAHVGCKTSCGYGVATGGPLAWGLCYNHEMSPSQSYCDDS 541
            F+     RN         F+ Y +FI A   +   GFGTTG    + +EI AF A    +T+ G+  A  GP AWG C+  E      YC  S
Sbjct:   87 FDQMLKHRNDNACQGKNNFYSYNAFINAARSFG--GFGTTGDTTARKREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPS 177

HSP 2 Score: 39.2762 bits (90), Expect = 5.864e-14
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 1 
Query:  553 YPCTPGAEYYARGAIPIYWNYNYGATG 633
            +PC PG +Y+ RG I I  NYNYG  G
Sbjct:  180 WPCAPGRKYFGRGPIQISHNYNYGPCG 206
The following BLAST results are available for this feature:
BLAST of FC876330 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 35
Match NameE-valueIdentityDescription
CHI1_TOBAC8.851e-1634.78Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1... [more]
CHI2_TOBAC8.856e-1634.78Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1... [more]
CHI2_PEA1.944e-1532.35Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=... [more]
CHIC_SOLLC7.205e-1535.48Basic 30 kDa endochitinase OS=Solanum lycopersicum... [more]
CHI2_SOLTU9.367e-1535.48Endochitinase 2 (Fragment) OS=Solanum tuberosum GN... [more]
CHI1_SOLTU9.367e-1535.48Endochitinase 1 (Fragment) OS=Solanum tuberosum GN... [more]
CHI9_ORYSJ2.051e-1432.58Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHI3_SOLTU2.670e-1435.48Endochitinase 3 (Fragment) OS=Solanum tuberosum GN... [more]
CHIT_SOLTU4.501e-1433.70Endochitinase OS=Solanum tuberosum PE=2 SV=1[more]
CHI4_SOLTU5.864e-1434.41Endochitinase 4 (Fragment) OS=Solanum tuberosum GN... [more]

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Properties
Property NameValue
Genbank descriptionC32003E01EF DevOvary1 Citrus clementina cDNA clone C32003E01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC876330 ID=FC876330; Name=FC876330; organism=Citrus clementina; type=EST; length=634bp
GATCTTCCCCCAAGTGAATCTCGCAACAACAGGTAGATCTGGTGAGAACG
AGAAAATAAAGATGAAGAATGGCAGTGTGGCAATGACACTGACAGTGGCA
CTAGTACTGGCACTGGCAGTGAGTTTGGCAAATGGAGATGAATCAGAGAA
GGTGATAGTGAAGAAGGTGAAGGGGAAAAAAGTTTGCATAAAAGGGTGGG
AGTGCCCAACATGGTCGAAATTCTGCTGTAACGAGACGATATCAGACTAT
TTCCAGGTTTACCAGTTTGAGAATTTCTTCTCGAAAAGGAACACGCCGGT
GGCACATGCCGTGGGCTTCTGGGATTATCAGTCTTTCATTACTGCCACCG
TCAAATATCAGCCCCTCGGCTTCGGTACCACCGGTACTAAGCTTGATAAG
ATGAAGGAGATTTGCGCTTTTCTTGCCCACGTCGGCTGCAAGACCTCTTG
TGGATATGGGGTGGCCACTGGAGGACCATTGGCCTGGGGACTTTGCTACA
ACCATGAAATGAGCCCCAGCCAGTCATATTGTGATGATTCCTACAATACA
CATATCCCTGCACCCCTGGAGCTGAGTACTACGCCCGAGGTGCTATACCT
ATATACTGGAACTATAACTATGGAGCTACTGGGG
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