FC920668

Overview
NameFC920668
Unique NameFC920668
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length688
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig10260 contig Ccv1_Contig10260:6..694. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHS1_CASGL (Chalcone synthase OS=Casuarina glauca GN=CHS1 PE=2 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 2.689e-101
Identity = 160/182 (87.91%), Postives = 173/182 (95.05%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNR 598
            M TV+E+R AQRA+GPATVLAIGTATP + ++Q+ YPDYYFRIT SEH TELKEKF+RMCDKSMIKKRYMYLTEEILKE+PNMCAYMAPSLDARQD+VVVE+PKLGKEAA KAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTVLRLAKDLAENNR
Sbjct:    1 MVTVEEVRKAQRAEGPATVLAIGTATPPNCLDQSTYPDYYFRITNSEHKTELKEKFQRMCDKSMIKKRYMYLTEEILKEHPNMCAYMAPSLDARQDMVVVEIPKLGKEAAVKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAKDLAENNR 182

HSP 2 Score: 56.225 bits (134), Expect = 2.689e-101
Identity = 26/28 (92.86%), Postives = 28/28 (100.00%), Query Frame = 1 
Query:  595 QGARVLVVCSEITAVTFRGPADTHLDSL 678
            +GARVLVVCSEITAVTFRGP+DTHLDSL
Sbjct:  182 RGARVLVVCSEITAVTFRGPSDTHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHSY_PUELO (Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 SV=1)

HSP 1 Score: 333.183 bits (853), Expect = 5.971e-101
Identity = 161/182 (88.46%), Postives = 172/182 (94.51%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNR 598
            M +V EIR AQRA+GPAT+LAIGTA P + V+Q+ YPDYYFRIT SEHMTELKEKF+RMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTK +GLRP VKR+MMYQQGCFAGGTVLRLAKDLAENN+
Sbjct:    1 MVSVAEIRQAQRAEGPATILAIGTANPPNCVDQSTYPDYYFRITNSEHMTELKEKFQRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKQLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNK 182

HSP 2 Score: 56.225 bits (134), Expect = 5.971e-101
Identity = 26/28 (92.86%), Postives = 28/28 (100.00%), Query Frame = 1 
Query:  595 QGARVLVVCSEITAVTFRGPADTHLDSL 678
            +GARVLVVCSEITAVTFRGP+DTHLDSL
Sbjct:  182 KGARVLVVCSEITAVTFRGPSDTHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHSY_PHAVU (Chalcone synthase 17 OS=Phaseolus vulgaris GN=CHS17 PE=2 SV=1)

HSP 1 Score: 332.798 bits (852), Expect = 7.790e-101
Identity = 160/182 (87.91%), Postives = 173/182 (95.05%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNR 598
            M +V EIR AQRA+GPAT+LAIGTATP++ V+Q+ YPDYYFRIT SEHMT+LKEKF+RMCDKSMIKKRYM+L EEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRP VKR+MMYQQGCFAGGTVLRLAKDLAENN+
Sbjct:    1 MVSVSEIRQAQRAEGPATILAIGTATPSNCVDQSTYPDYYFRITNSEHMTDLKEKFQRMCDKSMIKKRYMHLNEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNK 182

HSP 2 Score: 56.225 bits (134), Expect = 7.790e-101
Identity = 26/28 (92.86%), Postives = 28/28 (100.00%), Query Frame = 1 
Query:  595 QGARVLVVCSEITAVTFRGPADTHLDSL 678
            +GARVLVVCSEITAVTFRGP+DTHLDSL
Sbjct:  182 KGARVLVVCSEITAVTFRGPSDTHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHSY_ANTMA (Chalcone synthase OS=Antirrhinum majus GN=CHS PE=3 SV=1)

HSP 1 Score: 332.028 bits (850), Expect = 7.790e-101
Identity = 160/181 (88.40%), Postives = 172/181 (95.03%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENN 595
            M TV+E+R AQRA+GPATVLAIGTATPA+ V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKS IK+RYM+LTEEILKENP MC YMAPSLDARQDIVVVEVP+LGKEAA KAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKRFMMYQQGCFAGGTVLR+AKDLAENN
Sbjct:    1 MVTVEEVRRAQRAEGPATVLAIGTATPANCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSNIKRRYMHLTEEILKENPAMCEYMAPSLDARQDIVVVEVPRLGKEAAQKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRMAKDLAENN 181

HSP 2 Score: 56.9954 bits (136), Expect = 7.790e-101
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:  598 GARVLVVCSEITAVTFRGPADTHLDSL 678
            GARVLVVCSEITAVTFRGPADTHLDSL
Sbjct:  183 GARVLVVCSEITAVTFRGPADTHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHSY_VITVI (Chalcone synthase OS=Vitis vinifera GN=CHS PE=2 SV=1)

HSP 1 Score: 333.954 bits (855), Expect = 1.016e-100
Identity = 162/181 (89.50%), Postives = 172/181 (95.03%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENN 595
            M +V EIR AQRA+GPATVLAIGTATPA+ V QADYPDYYFRIT SEHMTELKEKFKRMC+KSMI KRYM+LTEEILKENPN+CAYMAPSLDARQD+VVVEVPKLGKEAA KAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GL+PSVKR MMYQQGCFAGGTVLRLAKDLAENN
Sbjct:    1 MVSVAEIRKAQRAEGPATVLAIGTATPANCVYQADYPDYYFRITNSEHMTELKEKFKRMCEKSMINKRYMHLTEEILKENPNVCAYMAPSLDARQDMVVVEVPKLGKEAAAKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLKPSVKRLMMYQQGCFAGGTVLRLAKDLAENN 181

HSP 2 Score: 54.6842 bits (130), Expect = 1.016e-100
Identity = 25/27 (92.59%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:  598 GARVLVVCSEITAVTFRGPADTHLDSL 678
            G+RVLVVCSEITAVTFRGP+DTHLDSL
Sbjct:  183 GSRVLVVCSEITAVTFRGPSDTHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHS1_CAMSI (Chalcone synthase 1 OS=Camellia sinensis GN=CHS1 PE=2 SV=1)

HSP 1 Score: 330.872 bits (847), Expect = 2.944e-100
Identity = 159/182 (87.36%), Postives = 171/182 (93.96%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNR 598
            M TV++IR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEH  ELKEKFKRMCDKSMIKKRYMYLTEEILKENP +C YMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTVLRLAKDLAENN+
Sbjct:    1 MVTVEDIRRAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHKAELKEKFKRMCDKSMIKKRYMYLTEEILKENPQVCEYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAKDLAENNK 182

HSP 2 Score: 56.225 bits (134), Expect = 2.944e-100
Identity = 26/28 (92.86%), Postives = 28/28 (100.00%), Query Frame = 1 
Query:  595 QGARVLVVCSEITAVTFRGPADTHLDSL 678
            +GARVLVVCSEITAVTFRGP+DTHLDSL
Sbjct:  182 KGARVLVVCSEITAVTFRGPSDTHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHS6_SOYBN (Chalcone synthase 6 OS=Glycine max GN=CHS6 PE=3 SV=1)

HSP 1 Score: 330.872 bits (847), Expect = 3.842e-100
Identity = 159/181 (87.85%), Postives = 172/181 (95.03%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENN 595
            M +V+EIR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT S+HM ELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYM PSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAENN
Sbjct:    1 MVSVEEIRKAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSDHMNELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMEPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENN 181

HSP 2 Score: 55.8398 bits (133), Expect = 3.842e-100
Identity = 26/27 (96.30%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:  598 GARVLVVCSEITAVTFRGPADTHLDSL 678
            GARVLVVCSEITAVTFRGP+DTHLDSL
Sbjct:  183 GARVLVVCSEITAVTFRGPSDTHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHSE_IPONI (Chalcone synthase E OS=Ipomoea nil GN=CHSE PE=2 SV=1)

HSP 1 Score: 332.028 bits (850), Expect = 5.012e-100
Identity = 157/182 (86.26%), Postives = 173/182 (95.05%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNR 598
            M TV+E+R AQRA GPAT++AIGT+TP + V+Q+ YPDYYFRIT SEH+ ELKEKFKRMC+KSMIKKRYMYLTEEILKENPN+CAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GL+PSVKRFMMYQQGCFAGGTV+RLAKDLAENN+
Sbjct:    1 MVTVEEVRKAQRAQGPATIMAIGTSTPQNCVDQSTYPDYYFRITNSEHLVELKEKFKRMCEKSMIKKRYMYLTEEILKENPNICAYMAPSLDARQDIVVVEVPKLGKEAAQKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLQPSVKRFMMYQQGCFAGGTVIRLAKDLAENNK 182

HSP 2 Score: 54.299 bits (129), Expect = 5.012e-100
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = 1 
Query:  595 QGARVLVVCSEITAVTFRGPADTHLDSL 678
            +GARVLVVCSEITAVTFRGP+D HLDSL
Sbjct:  182 KGARVLVVCSEITAVTFRGPSDAHLDSL 209
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHS4_SORBI (Chalcone synthase 4 OS=Sorghum bicolor GN=CHS4 PE=3 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 6.537e-100
Identity = 161/180 (89.44%), Postives = 171/180 (95.00%), Query Frame = 2 
Query:   59 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNR 598
            TV+E+R AQRA GPATVLAIGTATPA+ V+QADYPDYYFRITKSEHMTELKEKFKRMCDKS I+KRYM+LTEE L ENPNMCAYMAPSLDARQDIVVVEVPKLGK AA KAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTK++GLRPSVKR MMYQQGCFAGGTVLR+AKDLAENNR
Sbjct:    7 TVEEVRKAQRATGPATVLAIGTATPANCVHQADYPDYYFRITKSEHMTELKEKFKRMCDKSQIRKRYMHLTEEYLAENPNMCAYMAPSLDARQDIVVVEVPKLGKAAAQKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKMLGLRPSVKRLMMYQQGCFAGGTVLRVAKDLAENNR 186

HSP 2 Score: 52.373 bits (124), Expect = 6.537e-100
Identity = 23/28 (82.14%), Postives = 28/28 (100.00%), Query Frame = 1 
Query:  595 QGARVLVVCSEITAVTFRGPADTHLDSL 678
            +GARVLVVCSEITAVTFRGP+++HLDS+
Sbjct:  186 RGARVLVVCSEITAVTFRGPSESHLDSM 213
[Close]
BLAST of FC920668 vs. ExPASy Swiss-Prot
Match: CHS2_SOLLC (Chalcone synthase 2 OS=Solanum lycopersicum GN=CHS2 PE=2 SV=2)

HSP 1 Score: 330.487 bits (846), Expect = 6.539e-100
Identity = 157/182 (86.26%), Postives = 172/182 (94.51%), Query Frame = 2 
Query:   53 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNR 598
            M TV+E+R AQRA GPAT++AIGTATP++ V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMI KRYM+LTEEILKENPN+C YMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITH++FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTV+RLAKDLAENN+
Sbjct:    1 MVTVEEVRRAQRAKGPATIMAIGTATPSNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMINKRYMHLTEEILKENPNICEYMAPSLDARQDIVVVEVPKLGKEAAQKAIKEWGQPKSKITHVVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVIRLAKDLAENNK 182

HSP 2 Score: 55.4546 bits (132), Expect = 6.539e-100
Identity = 25/28 (89.29%), Postives = 28/28 (100.00%), Query Frame = 1 
Query:  595 QGARVLVVCSEITAVTFRGPADTHLDSL 678
            +GARVLVVCSEITAVTFRGP+DTHLDS+
Sbjct:  182 KGARVLVVCSEITAVTFRGPSDTHLDSM 209
The following BLAST results are available for this feature:
BLAST of FC920668 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 145
Match NameE-valueIdentityDescription
CHS1_CASGL2.689e-10187.91Chalcone synthase OS=Casuarina glauca GN=CHS1 PE=2... [more]
CHSY_PUELO5.971e-10188.46Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 S... [more]
CHSY_PHAVU7.790e-10187.91Chalcone synthase 17 OS=Phaseolus vulgaris GN=CHS1... [more]
CHSY_ANTMA7.790e-10188.40Chalcone synthase OS=Antirrhinum majus GN=CHS PE=3... [more]
CHSY_VITVI1.016e-10089.50Chalcone synthase OS=Vitis vinifera GN=CHS PE=2 SV... [more]
CHS1_CAMSI2.944e-10087.36Chalcone synthase 1 OS=Camellia sinensis GN=CHS1 P... [more]
CHS6_SOYBN3.842e-10087.85Chalcone synthase 6 OS=Glycine max GN=CHS6 PE=3 SV... [more]
CHSE_IPONI5.012e-10086.26Chalcone synthase E OS=Ipomoea nil GN=CHSE PE=2 SV... [more]
CHS4_SORBI6.537e-10089.44Chalcone synthase 4 OS=Sorghum bicolor GN=CHS4 PE=... [more]
CHS2_SOLLC6.539e-10086.26Chalcone synthase 2 OS=Solanum lycopersicum GN=CHS... [more]

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Properties
Property NameValue
Genbank descriptionC32011E03EF RVDevelop1 Citrus clementina cDNA clone C32011E03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920668 ID=FC920668; Name=FC920668; organism=Citrus clementina; type=EST; length=688bp
GGAACTCACCAAGCACGAGCCACCAACATATTCATTAAGGGTTCAACTTG
AAATGGCAACCGTTCAAGAGATCAGAAACGCTCAGCGTGCCGACGGCCCG
GCCACCGTCCTCGCCATCGGTACGGCCACGCCTGCCCACAGTGTCAACCA
GGCTGATTATCCCGACTATTACTTCAGGATCACAAAGAGCGAGCATATGA
CGGAGCTTAAAGAGAAGTTCAAGCGCATGTGTGACAAGTCGATGATTAAG
AAGCGTTACATGTACTTAACGGAAGAGATTTTGAAGGAAAACCCCAACAT
GTGCGCTTACATGGCTCCATCACTCGACGCACGTCAAGACATTGTGGTGG
TCGAAGTGCCGAAGCTCGGGAAAGAAGCTGCTACAAAGGCCATCAAAGAA
TGGGGCCAGCCCAAGTCTAAGATCACCCACCTCATCTTCTGCACCACCTC
CGGCGTCGACATGCCCGGCGCCGACTACCAGCTCACCAAACTGATCGGCC
TGCGCCCCTCCGTCAAGCGCTTCATGATGTACCAACAAGGATGTTTCGCC
GGCGGCACTGTTCTTCGCCTCGCTAAAGACTTGGCTGAGAACAACAGGGC
GCTCGCGTTCTTGTTGTCTGTTCGGAGATCACGGCAGTCACTTTCCGCGG
CCCTGCCGATACTCATCTTGATTCTCTTTGTGAGCCAG
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