BQ623101

Overview
NameBQ623101
Unique NameBQ623101
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length646
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12771 contig Csv1_Contig12771:132..777. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_GEOLS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=ispF PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/132 (48.48%), Postives = 92/132 (69.70%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHG+D+HRL     LI+GG+++P E+G   HSD DVLLH + DA+LGAL + DIG+ FPD+DP +KGA S   ++  V L+   GY +GNLDAT+I QRPK++P+ + +R N++   G +    N+KA T
Sbjct:    2 RIGHGYDVHRLVEDRKLIMGGVDIPWEKGLLGHSDADVLLHAIADALLGALAMGDIGKHFPDTDPAFKGADSMKLLEHVVGLIRTQGYAVGNLDATIIAQRPKMAPHIQAMRENVARACGVEVDRINVKATT 133
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURPS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia pseudomallei GN=ispF PE=1 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURP6 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia pseudomallei (strain 668) GN=ispF PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURP1 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia pseudomallei (strain 1710b) GN=ispF PE=1 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURP0 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia pseudomallei (strain 1106a) GN=ispF PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURMS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia mallei (strain SAVP1) GN=ispF PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURMA (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia mallei GN=ispF PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURM9 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia mallei (strain NCTC 10229) GN=ispF PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_BURM7 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Burkholderia mallei (strain NCTC 10247) GN=ispF PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.950e-32
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 3 
Query:  237 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKTH 644
            + FR+G G+D+H+L PG PLIIGG+ +P+ERG   HSD DVLLH + DA+ GA  L DIG+ F D+DP++KGA S   ++E    + +AG+ I N+D+T+I Q PKL+P+ + +R N++  L       N+KAKT+
Sbjct:    1 MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTN 136
[Close]
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Match: ISPF_SHESH (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sediminis (strain HAW-EB3) GN=ispF PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.546e-32
Identity = 65/132 (49.24%), Postives = 91/132 (68.94%), Query Frame = 3 
Query:  246 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPPGGNLKAKT 641
            R+GHGFD+H+   G PL++GG+++P+E G  AHSDGDV+LH + DA+LGA+ L DIG+ FPD+D ++KGA S   ++   RL  E GY +GNLD T+I Q PK++P+ E IR  L+     D    N+KA T
Sbjct:    4 RIGHGFDVHKFGGGSPLLLGGVHIPYETGLIAHSDGDVVLHAISDAVLGAVALGDIGKHFPDTDEEFKGADSRHLLRHCYRLAKEKGYSMGNLDVTIIAQAPKMAPHIEAIREVLALDFETDTDNINVKATT 135
The following BLAST results are available for this feature:
BLAST of BQ623101 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ISPF_GEOLS1.950e-3248.482-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURPS1.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURP61.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURP11.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURP01.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURMS1.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURMA1.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURM91.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_BURM71.950e-3247.062-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHESH2.546e-3249.242-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00192 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00192 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623101 ID=BQ623101; Name=BQ623101; organism=Citrus sinensis; type=EST; length=646bp
AGATGGTGTTGACGATGGCCGCTCAATCATACACCACCACTCCTCTCCAC
AGCAAAATTACGAATAAGCCACTATGTCCTCCTCCGTTGAGTTTGAAGCC
TCGTAGCTTAACCGCCAAACATCTCCGCACAACACAGTCAACGTCGCTGC
CTCGTATTTCAGTTTCAGCTGCAGCCATTTCTTCCATCGAAGTTAAAGAG
TCATCGGCTTCGATCCAACCGTCCAAATCAAAATCACTGCCTTTTCGTGT
GGGCCACGGGTTTGATCTTCATAGATTGGAGCCTGGCTACCCTCTGATCA
TCGGTGGAATCAATGTTCCCCACGAGAGAGGCTGCGAGGCTCACTCTGAC
GGTGATGTTTTGCTTCACTGTGTTGTGGATGCAATTTTGGGAGCATTGGG
TCTTCCTGATATTGGTCAGATATTTCCTGATTCTGATCCTAAGTGGAAGG
GTGCACCATCCTCTGTTTTTATCAAAGAAGCTGTGAGACTCATGGATGAG
GCCGGCTATGAGATTGGCAACTTGGATGCCACGCTGATTCTTCAAAGACC
AAAATTGAGCCCATACAAGGAGACTATCCGAACCAACTTGTCTGAACTGC
TAGGAGCAGACCCCCCCGGTGGAAATCTGAAAGCGAAAACTCATGA
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