CB305012

Overview
NameCB305012
Unique NameCB305012
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length579
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2325 contig Csv1_Contig2325:131..710. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_NEUCR (Elongation factor 1-alpha OS=Neurospora crassa GN=tef-1 PE=3 SV=2)

HSP 1 Score: 116.316 bits (290), Expect = 9.857e-42
Identity = 55/73 (75.34%), Postives = 65/73 (89.04%), Query Frame = -1 
Query:  226 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEK 444
            KF+EL+ KIDRR+GK +E  PKF+K+GDA +VKMIP+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK V+K
Sbjct:  370 KFAELLEKIDRRTGKAVEASPKFIKSGDAAIVKMIPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVDK 442

HSP 2 Score: 73.559 bits (179), Expect = 9.857e-42
Identity = 31/44 (70.45%), Postives = 39/44 (88.64%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP   AA+FT+QVI++NHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPAGAASFTAQVIVLNHPGQVGAGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_AURPU (Elongation factor 1-alpha OS=Aureobasidium pullulans GN=TEF1 PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 1.284e-41
Identity = 57/75 (76.00%), Postives = 66/75 (88.00%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KFSELV KIDRR+GK +E  PKF+K+GDA +VKM+P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+V K D
Sbjct:  368 KFSELVEKIDRRTGKSVEAAPKFIKSGDAAIVKMVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVAKSD 442

HSP 2 Score: 68.9366 bits (167), Expect = 1.284e-41
Identity = 28/44 (63.64%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +   +F +QVI++NHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  325 DSKNDPPKGCDSFNAQVIVLNHPGQVGAGYAPVLDCHTAHIACK 368
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_PODAN (Elongation factor 1-alpha OS=Podospora anserina GN=TEF PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.284e-41
Identity = 55/73 (75.34%), Postives = 66/73 (90.41%), Query Frame = -1 
Query:  226 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEK 444
            KFSEL+ KIDRR+GK +E+ PKF+K+GDA +VKM+P+KPM VE F++YPPLGRFAVRDMRQTVAVGVIK VEK
Sbjct:  370 KFSELLQKIDRRTGKAVEESPKFIKSGDAAIVKMVPSKPMCVEAFTEYPPLGRFAVRDMRQTVAVGVIKKVEK 442

HSP 2 Score: 71.2478 bits (173), Expect = 1.284e-41
Identity = 30/44 (68.18%), Postives = 38/44 (86.36%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP   AA+F +QVI++NHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPMGAASFDAQVIVLNHPGQVGAGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_AJECG (Elongation factor 1-alpha OS=Ajellomyces capsulata (strain ATCC 26029 / G186AR / H82 / RMSCC 2432) GN=TEF PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.674e-41
Identity = 56/75 (74.67%), Postives = 66/75 (88.00%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KFSEL+ KIDRR+GK +E  PKF+K+GDA +VKM+P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+V K D
Sbjct:  370 KFSELIEKIDRRTGKSVENNPKFIKSGDAAIVKMVPSKPMCVEPFTDYPPLGRFAVRDMRQTVAVGVIKSVIKSD 444

HSP 2 Score: 69.3218 bits (168), Expect = 1.674e-41
Identity = 28/44 (63.64%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +   +F +QVI++NHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPKGCESFNAQVIVLNHPGQVGAGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_ASPOR (Elongation factor 1-alpha OS=Aspergillus oryzae GN=tef1 PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.674e-41
Identity = 55/73 (75.34%), Postives = 68/73 (93.15%), Query Frame = -1 
Query:  226 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEK 444
            KF+EL+ KIDRR+GK +E +PKF+K+GDA +VKMIP+KPM VE+F+D+PPLGRFAVRDMRQTVAVGVIK+VEK
Sbjct:  370 KFAELLEKIDRRTGKSVEDKPKFIKSGDAAIVKMIPSKPMCVESFTDFPPLGRFAVRDMRQTVAVGVIKSVEK 442

HSP 2 Score: 70.8626 bits (172), Expect = 1.674e-41
Identity = 29/44 (65.91%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP     +F +QVI++NHPGQ+GNGYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPAGCDSFNAQVIVLNHPGQVGNGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_SORMA (Elongation factor 1-alpha OS=Sordaria macrospora GN=TEF PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.674e-41
Identity = 55/73 (75.34%), Postives = 65/73 (89.04%), Query Frame = -1 
Query:  226 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEK 444
            KF+EL+ KIDRR+GK +E  PKF+K+GDA +VKMIP+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK V+K
Sbjct:  370 KFAELLEKIDRRTGKAVETSPKFIKSGDAAIVKMIPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVDK 442

HSP 2 Score: 72.7886 bits (177), Expect = 1.674e-41
Identity = 31/44 (70.45%), Postives = 39/44 (88.64%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP   AA+FT+QVI++NHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPVGAASFTAQVIVLNHPGQVGAGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_CANAL (Elongation factor 1-alpha OS=Candida albicans GN=TEF1 PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.181e-41
Identity = 58/75 (77.33%), Postives = 67/75 (89.33%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF  LV KIDRR+GK+LE+ PKF+K+GDA +VKM+PTKPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+VEK D
Sbjct:  369 KFDTLVEKIDRRTGKKLEENPKFVKSGDAAIVKMVPTKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVEKSD 443

HSP 2 Score: 65.855 bits (159), Expect = 2.181e-41
Identity = 27/44 (61.36%), Postives = 36/44 (81.82%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +   +F +QVI++NHPGQI  GY+PVLDCHT+HIA K
Sbjct:  326 DSKNDPPKGCDSFNAQVIVLNHPGQISAGYSPVLDCHTAHIACK 369
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_PODCU (Elongation factor 1-alpha OS=Podospora curvicolla GN=TEF PE=3 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 2.841e-41
Identity = 55/73 (75.34%), Postives = 66/73 (90.41%), Query Frame = -1 
Query:  226 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEK 444
            KF+EL+ KIDRR+GK +E+ PKF+K+GDA +VKMIP+KPM VE F++YPPLGRFAVRDMRQTVAVGVIK VEK
Sbjct:  370 KFAELLQKIDRRTGKAVEESPKFIKSGDAAIVKMIPSKPMCVEAFTEYPPLGRFAVRDMRQTVAVGVIKKVEK 442

HSP 2 Score: 70.8626 bits (172), Expect = 2.841e-41
Identity = 30/44 (68.18%), Postives = 38/44 (86.36%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP   AA+F +QVI++NHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPLGAASFDAQVIVLNHPGQVGAGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_STYLE (Elongation factor 1-alpha OS=Stylonychia lemnae GN=EFAA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 2.842e-41
Identity = 52/75 (69.33%), Postives = 65/75 (86.67%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF E+ +K+DRRSGK LE+EPKF+K+G+A +V+M+P KPM VE F+ YPPLGRFAVRDM+QTVAVGVIK V KK+
Sbjct:  359 KFDEIESKVDRRSGKVLEEEPKFIKSGEAALVRMVPQKPMCVEAFNQYPPLGRFAVRDMKQTVAVGVIKEVVKKE 433

HSP 2 Score: 74.3294 bits (181), Expect = 2.842e-41
Identity = 31/45 (68.89%), Postives = 39/45 (86.67%), Query Frame = -3 
Query:  443 SNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 577
            S+SK+DPA++  NF +QVI++NHPGQI  GYAPVLDCHT+HIA K
Sbjct:  315 SDSKNDPAKDTTNFLAQVIVLNHPGQIQKGYAPVLDCHTAHIACK 359
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_PUCGR (Elongation factor 1-alpha OS=Puccinia graminis GN=TEF PE=3 SV=2)

HSP 1 Score: 113.235 bits (282), Expect = 3.702e-41
Identity = 55/75 (73.33%), Postives = 64/75 (85.33%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF  L  KIDRR+GK LE+ PKF+K+GDA +VKM+P+K M VE F+DYPPLGRFAVRDMRQTVAVGVIK+VEK D
Sbjct:  368 KFDTLQQKIDRRTGKALEEAPKFVKSGDACLVKMVPSKAMCVEPFADYPPLGRFAVRDMRQTVAVGVIKSVEKTD 442

HSP 2 Score: 74.7146 bits (182), Expect = 3.702e-41
Identity = 31/44 (70.45%), Postives = 39/44 (88.64%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            ++K DP +EAA+F +QVI++NHPGQIGNGY PVLDCHT+HIA K
Sbjct:  325 DTKCDPPKEAASFVAQVIVLNHPGQIGNGYCPVLDCHTAHIACK 368
The following BLAST results are available for this feature:
BLAST of CB305012 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158
Match NameE-valueIdentityDescription
EF1A_NEUCR9.857e-4275.34Elongation factor 1-alpha OS=Neurospora crassa GN=... [more]
EF1A_AURPU1.284e-4176.00Elongation factor 1-alpha OS=Aureobasidium pullula... [more]
EF1A_PODAN1.284e-4175.34Elongation factor 1-alpha OS=Podospora anserina GN... [more]
EF1A_AJECG1.674e-4174.67Elongation factor 1-alpha OS=Ajellomyces capsulata... [more]
EF1A_ASPOR1.674e-4175.34Elongation factor 1-alpha OS=Aspergillus oryzae GN... [more]
EF1A_SORMA1.674e-4175.34Elongation factor 1-alpha OS=Sordaria macrospora G... [more]
EF1A_CANAL2.181e-4177.33Elongation factor 1-alpha OS=Candida albicans GN=T... [more]
EF1A_PODCU2.841e-4175.34Elongation factor 1-alpha OS=Podospora curvicolla ... [more]
EF1A_STYLE2.842e-4169.33Elongation factor 1-alpha OS=Stylonychia lemnae GN... [more]
EF1A_PUCGR3.702e-4173.33Elongation factor 1-alpha OS=Puccinia graminis GN=... [more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0003_IIaR_G01 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0003_IIaR_G01 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB305012 ID=CB305012; Name=CB305012; organism=Citrus sinensis; type=EST; length=579bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAACTTACCAAAAAAAAAC
TCTGATATAAAACCAAATTCAGAGAATTAAAATGAAGTCCCGTAAAACAA
ACTAAAACACGACAAAAATAGCATAAACAGAGAAAATAAAAGCTAACTAT
AGCAGATAGATCCCAAAAAACTCCTCACTTCTTCTTGGCTGCAGACTTGG
TGACCTTGGCACCTGTTGGATCCTTCTTTTCAACATTCTTAATCACACCA
ACAGCAACAGTCTGACGCATGTCCCTAACGGCAAAACGACCAAGAGGTGG
GTAGTCAGAGAAGGTTTCAACAACCATGGGCTTGGTGGGAATCATCTTAA
CCATTCCAGCATCTCCATTCTTCAAGAACTTGGGCTCCTTCTCAAGCTCC
TTGCCTGATCGCCTATCAATCTTCGTCACGAGCTCAGAAAACTTTGACGG
CAATGTGGGAGGTGTGGCAGTCAAGCACTGGGGCATATCCATTTCCAATC
TGGCCAGGGTGGTTCATGATGATGACCTGTGAGGTGAAGTTGGCAGCTTC
CCTGGCTGGATCATCCTTCGAGTTGGAAG
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