CB305012

Overview
NameCB305012
Unique NameCB305012
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length579
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2325 contig Csv1_Contig2325:131..710. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_EUGGR (Elongation factor 1-alpha OS=Euglena gracilis GN=TEF PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 3.703e-41
Identity = 52/75 (69.33%), Postives = 62/75 (82.67%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF+ + TKIDRRSGKELE EPKF+K+GDA +V M P KPM VE+F+DYPPLG  +  DMRQTVAVGVIK+V KK+
Sbjct:  359 KFATIQTKIDRRSGKELEAEPKFIKSGDAAIVLMKPQKPMCVESFTDYPPLG-VSCGDMRQTVAVGVIKSVNKKE 432

HSP 2 Score: 84.7297 bits (208), Expect = 3.703e-41
Identity = 37/45 (82.22%), Postives = 43/45 (95.56%), Query Frame = -3 
Query:  443 SNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 577
            SN+K+DPA+EAA+FT+QVII+NHPGQIGNGYAPVLDCHT HIA K
Sbjct:  315 SNAKNDPAKEAADFTAQVIILNHPGQIGNGYAPVLDCHTCHIACK 359
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_YARLI (Elongation factor 1-alpha OS=Yarrowia lipolytica GN=TEF PE=2 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 4.824e-41
Identity = 54/75 (72.00%), Postives = 66/75 (88.00%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF  L+ KIDRR+GK++E  PKF+K+GDA +VKM+P+KPM VE F++YPPLGRFAVRDMRQTVAVGVIK+VEK D
Sbjct:  370 KFDTLIEKIDRRTGKKMEDSPKFIKSGDAAIVKMVPSKPMCVEAFTEYPPLGRFAVRDMRQTVAVGVIKSVEKSD 444

HSP 2 Score: 69.3218 bits (168), Expect = 4.824e-41
Identity = 29/44 (65.91%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +   +F +QVI++NHPGQIG GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPKGCDSFNAQVIVLNHPGQIGAGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_PICGU (Elongation factor 1-alpha OS=Pichia guilliermondii GN=TEF1 PE=3 SV=2)

HSP 1 Score: 118.627 bits (296), Expect = 1.067e-40
Identity = 55/75 (73.33%), Postives = 66/75 (88.00%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF  L+ KIDRR+GK++E  PKF+K+GDA +VKM+P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+VEK D
Sbjct:  369 KFDTLLEKIDRRTGKKMEDNPKFVKSGDASIVKMVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVEKSD 443

HSP 2 Score: 67.781 bits (164), Expect = 1.067e-40
Identity = 28/44 (63.64%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +   +FT+QVI++NHPGQI  GY+PVLDCHT+HIA K
Sbjct:  326 DSKNDPPKGCDSFTAQVIVLNHPGQISAGYSPVLDCHTAHIACK 369
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1AC_PORPU (Elongation factor 1-alpha C OS=Porphyra purpurea GN=TEF-C PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.067e-40
Identity = 56/75 (74.67%), Postives = 66/75 (88.00%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KFSEL+ K+DRRSGK+LE  PK +K+GDA MVKM+ +KPM VE F+ YPPLGRFAVRDMRQTVAVGVIK+VEKK+
Sbjct:  363 KFSELILKMDRRSGKKLEDSPKMIKSGDAAMVKMVASKPMCVEAFTSYPPLGRFAVRDMRQTVAVGVIKSVEKKE 437

HSP 2 Score: 67.781 bits (164), Expect = 1.067e-40
Identity = 29/44 (65.91%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +  A+F +QVII+NHPG+I  GYAPVLDCHT+HIA K
Sbjct:  320 DSKNDPPKGCASFNAQVIILNHPGEIHAGYAPVLDCHTAHIACK 363
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_ARXAD (Elongation factor 1-alpha OS=Arxula adeninivorans GN=TEF PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.390e-40
Identity = 56/75 (74.67%), Postives = 67/75 (89.33%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            +F EL+ KIDRRSGK++E  PKF+K GDA +V+MIP+KPM VETF++YPPLGRFAVRDMRQTVAVGVIK+VEK D
Sbjct:  369 RFDELIEKIDRRSGKKVEDSPKFVKAGDAAIVRMIPSKPMCVETFTEYPPLGRFAVRDMRQTVAVGVIKSVEKSD 443

HSP 2 Score: 66.2402 bits (160), Expect = 1.390e-40
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +   +FT+QVII NHPGQI  GY+PVLDCHT+HIA +
Sbjct:  326 DSKNDPPKGCDSFTAQVIIFNHPGQISAGYSPVLDCHTAHIACR 369
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_PORYE (Elongation factor 1-alpha OS=Porphyra yezoensis PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.391e-40
Identity = 56/75 (74.67%), Postives = 66/75 (88.00%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KFSEL+ K+DRRSGK+LE  PK +K+GDA MVKM+ +KPM VE F+ YPPLGRFAVRDMRQTVAVGVIK+VEKK+
Sbjct:  363 KFSELILKMDRRSGKKLEDTPKMIKSGDAAMVKMVASKPMCVEAFTQYPPLGRFAVRDMRQTVAVGVIKSVEKKE 437

HSP 2 Score: 67.781 bits (164), Expect = 1.391e-40
Identity = 29/44 (65.91%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +  A+F +QVII+NHPG+I  GYAPVLDCHT+HIA K
Sbjct:  320 DSKNDPPKGCASFNAQVIILNHPGEIHAGYAPVLDCHTAHIACK 363
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A2_SCHPO (Elongation factor 1-alpha-B/C OS=Schizosaccharomyces pombe GN=tef1b PE=1 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.360e-40
Identity = 55/76 (72.37%), Postives = 66/76 (86.84%), Query Frame = -1 
Query:  217 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKDP 444
            KF+EL+ KIDRRSGK++E+ PKF+K+GDA + KM+P+KPM VE F+DY PLGRFAVRDMRQTVAVGVIK VEK  P
Sbjct:  369 KFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAP 444

HSP 2 Score: 69.3218 bits (168), Expect = 2.360e-40
Identity = 30/44 (68.18%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP    A+FT+QVII+NHPGQI  GY+PVLDCHT+HIA K
Sbjct:  326 DSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACK 369
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A1_SCHPO (Elongation factor 1-alpha-A OS=Schizosaccharomyces pombe GN=tef1a PE=1 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 2.360e-40
Identity = 55/76 (72.37%), Postives = 66/76 (86.84%), Query Frame = -1 
Query:  217 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKDP 444
            KF+EL+ KIDRRSGK++E+ PKF+K+GDA + KM+P+KPM VE F+DY PLGRFAVRDMRQTVAVGVIK VEK  P
Sbjct:  369 KFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAP 444

HSP 2 Score: 69.3218 bits (168), Expect = 2.360e-40
Identity = 30/44 (68.18%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP    A+FT+QVII+NHPGQI  GY+PVLDCHT+HIA K
Sbjct:  326 DSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACK 369
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_CRYNE (Elongation factor 1-alpha OS=Cryptococcus neoformans GN=TEF1 PE=2 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 2.360e-40
Identity = 51/75 (68.00%), Postives = 66/75 (88.00%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF+EL+ KIDRR+GK +E  PKF+K+GDA +VK++  KP+ VET++DYPPLGRFAVRDMRQTVAVGVIK+V+K +
Sbjct:  369 KFAELIEKIDRRTGKVMEAAPKFVKSGDAAIVKLVAQKPLCVETYADYPPLGRFAVRDMRQTVAVGVIKSVDKTE 443

HSP 2 Score: 73.1738 bits (178), Expect = 2.360e-40
Identity = 31/44 (70.45%), Postives = 38/44 (86.36%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP  EAA+F +QVI++NHPGQIG GY PVLDCHT+HIA K
Sbjct:  326 DSKNDPPMEAASFNAQVIVLNHPGQIGAGYTPVLDCHTAHIACK 369
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_ARTSA (Elongation factor 1-alpha OS=Artemia salina PE=1 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 3.075e-40
Identity = 52/76 (68.42%), Postives = 64/76 (84.21%), Query Frame = -1 
Query:  217 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKDP 444
            KF+E+  K DRR+GK  E EPKF+K+GDA M+ ++P+KP+ VE FSD+PPLGRFAVRDMRQTVAVGVIK+V  KDP
Sbjct:  371 KFAEIKEKCDRRTGKTTEAEPKFIKSGDAAMITLVPSKPLCVEAFSDFPPLGRFAVRDMRQTVAVGVIKSVNFKDP 446

HSP 2 Score: 71.2478 bits (173), Expect = 3.075e-40
Identity = 30/45 (66.67%), Postives = 39/45 (86.67%), Query Frame = -3 
Query:  443 SNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 577
            S+SK++PAR + +F +QVI++NHPGQI NGY PVLDCHT+HIA K
Sbjct:  327 SDSKNNPARGSQDFFAQVIVLNHPGQISNGYTPVLDCHTAHIACK 371
The following BLAST results are available for this feature:
BLAST of CB305012 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158
Match NameE-valueIdentityDescription
EF1A_EUGGR3.703e-4169.33Elongation factor 1-alpha OS=Euglena gracilis GN=T... [more]
EF1A_YARLI4.824e-4172.00Elongation factor 1-alpha OS=Yarrowia lipolytica G... [more]
EF1A_PICGU1.067e-4073.33Elongation factor 1-alpha OS=Pichia guilliermondii... [more]
EF1AC_PORPU1.067e-4074.67Elongation factor 1-alpha C OS=Porphyra purpurea G... [more]
EF1A_ARXAD1.390e-4074.67Elongation factor 1-alpha OS=Arxula adeninivorans ... [more]
EF1A_PORYE1.391e-4074.67Elongation factor 1-alpha OS=Porphyra yezoensis PE... [more]
EF1A2_SCHPO2.360e-4072.37Elongation factor 1-alpha-B/C OS=Schizosaccharomyc... [more]
EF1A1_SCHPO2.360e-4072.37Elongation factor 1-alpha-A OS=Schizosaccharomyces... [more]
EF1A_CRYNE2.360e-4068.00Elongation factor 1-alpha OS=Cryptococcus neoforma... [more]
EF1A_ARTSA3.075e-4068.42Elongation factor 1-alpha OS=Artemia salina PE=1 S... [more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0003_IIaR_G01 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0003_IIaR_G01 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB305012 ID=CB305012; Name=CB305012; organism=Citrus sinensis; type=EST; length=579bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAACTTACCAAAAAAAAAC
TCTGATATAAAACCAAATTCAGAGAATTAAAATGAAGTCCCGTAAAACAA
ACTAAAACACGACAAAAATAGCATAAACAGAGAAAATAAAAGCTAACTAT
AGCAGATAGATCCCAAAAAACTCCTCACTTCTTCTTGGCTGCAGACTTGG
TGACCTTGGCACCTGTTGGATCCTTCTTTTCAACATTCTTAATCACACCA
ACAGCAACAGTCTGACGCATGTCCCTAACGGCAAAACGACCAAGAGGTGG
GTAGTCAGAGAAGGTTTCAACAACCATGGGCTTGGTGGGAATCATCTTAA
CCATTCCAGCATCTCCATTCTTCAAGAACTTGGGCTCCTTCTCAAGCTCC
TTGCCTGATCGCCTATCAATCTTCGTCACGAGCTCAGAAAACTTTGACGG
CAATGTGGGAGGTGTGGCAGTCAAGCACTGGGGCATATCCATTTCCAATC
TGGCCAGGGTGGTTCATGATGATGACCTGTGAGGTGAAGTTGGCAGCTTC
CCTGGCTGGATCATCCTTCGAGTTGGAAG
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