DR403235

Overview
NameDR403235
Unique NameDR403235
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length388
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DR403235_ssr11 microsatellite DR403235_ssr11:11..26. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2998 contig Csv1_Contig2998:1061..1449. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_SCLS1 (Autophagy-related protein 8 OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) GN=atg8 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.335e-32
Identity = 44/54 (81.48%), Postives = 51/54 (94.44%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KKYLVP+DLTVGQFVYV+RKRIKLS EKAIFIFV  +LPPTAA+MS+IYEE+K
Sbjct:   46 KKKYLVPSDLTVGQFVYVIRKRIKLSPEKAIFIFVDEVLPPTAALMSSIYEEHK 99

HSP 2 Score: 66.2402 bits (160), Expect = 2.335e-32
Identity = 31/46 (67.39%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+R+AE+ RIR+KY DRIPVI EK E+SDI  IDKK
Sbjct:    2 RSKFKDEHPFEKRKAEAERIRQKYSDRIPVICEKVEKSDIATIDKK 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_CHAGB (Autophagy-related protein 8 OS=Chaetomium globosum GN=ATG8 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.340e-32
Identity = 44/54 (81.48%), Postives = 51/54 (94.44%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KKYLVP+DLTVGQFVYV+RKRIKLS EKAIFIFV  +LPPTAA+MS+IYEE+K
Sbjct:   46 KKKYLVPSDLTVGQFVYVIRKRIKLSPEKAIFIFVDEVLPPTAALMSSIYEEHK 99

HSP 2 Score: 66.2402 bits (160), Expect = 2.340e-32
Identity = 31/46 (67.39%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+R+AE+ RIR+KY DRIPVI EK E+SDI  IDKK
Sbjct:    2 RSKFKDEHPFEKRKAEAERIRQKYADRIPVICEKVEKSDIATIDKK 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_EMENI (Autophagy-related protein 8 OS=Emericella nidulans GN=atg8 PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.347e-32
Identity = 45/54 (83.33%), Postives = 51/54 (94.44%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KKYLVPADLTVGQFVYV+RKRIKLS EKAIFIFV  +LPPTAA+MS+IYEE+K
Sbjct:   46 KKKYLVPADLTVGQFVYVIRKRIKLSPEKAIFIFVDEVLPPTAALMSSIYEEHK 99

HSP 2 Score: 65.0846 bits (157), Expect = 2.347e-32
Identity = 31/46 (67.39%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+R+AE+ RIR KY DRIPVI EK E+SDI  IDKK
Sbjct:    2 RSKFKDEHPFEKRKAEAERIRAKYADRIPVICEKVEKSDIATIDKK 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_BOTFB (Autophagy-related protein 8 OS=Botryotinia fuckeliana (strain B05.10) GN=atg8 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 3.041e-32
Identity = 44/54 (81.48%), Postives = 51/54 (94.44%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KKYLVP+DLTVGQFVYV+RKRIKLS EKAIFIFV  +LPPTAA+MS+IYEE+K
Sbjct:   46 KKKYLVPSDLTVGQFVYVIRKRIKLSPEKAIFIFVDEVLPPTAALMSSIYEEHK 99

HSP 2 Score: 65.855 bits (159), Expect = 3.041e-32
Identity = 31/46 (67.39%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+R+AE+ RIR+KY DRIPVI EK E+SDI  IDKK
Sbjct:    2 RSKFKDEHPFEKRKAEAERIRQKYNDRIPVICEKVEKSDIATIDKK 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_MAGGR (Autophagy-related protein 8 OS=Magnaporthe grisea GN=ATG8 PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.139e-31
Identity = 44/54 (81.48%), Postives = 51/54 (94.44%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KKYLVPADLTVGQFVYV+RKRIKLS EKAIFIFV++ LPPTAA+MS+IYE +K
Sbjct:   46 KKKYLVPADLTVGQFVYVIRKRIKLSPEKAIFIFVQDTLPPTAALMSSIYELHK 99

HSP 2 Score: 66.2402 bits (160), Expect = 1.139e-31
Identity = 31/46 (67.39%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+R+AE+ RIR+KY DRIPVI EK E+SDI  IDKK
Sbjct:    2 RSKFKDEHPFEKRKAEAERIRQKYTDRIPVICEKVEKSDIATIDKK 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_LODEL (Autophagy-related protein 8 OS=Lodderomyces elongisporus GN=ATG8 PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.915e-31
Identity = 42/54 (77.78%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP DLTVGQFVYV+RKRIKL +EKAIFIFV +ILPPTAA++S IYEE+K
Sbjct:   46 KRKYLVPVDLTVGQFVYVIRKRIKLPSEKAIFIFVNDILPPTAALISTIYEEHK 99

HSP 2 Score: 66.6254 bits (161), Expect = 1.915e-31
Identity = 29/46 (63.04%), Postives = 38/46 (82.61%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+RQAE+ARI +++ DR+PVI EK E SDIP+IDK+
Sbjct:    2 RSQFKDEHPFEKRQAEAARIAQRFKDRVPVICEKVENSDIPEIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_CANAL (Autophagy-related protein 8 OS=Candida albicans GN=ATG8 PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.921e-31
Identity = 42/54 (77.78%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP DLTVGQFVYV+RKRIKL +EKAIFIFV +ILPPTAA++S IYEE+K
Sbjct:   46 KRKYLVPVDLTVGQFVYVIRKRIKLPSEKAIFIFVNDILPPTAALISTIYEEHK 99

HSP 2 Score: 66.6254 bits (161), Expect = 1.921e-31
Identity = 29/46 (63.04%), Postives = 38/46 (82.61%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+RQAE+ARI +++ DR+PVI EK E SDIP+IDK+
Sbjct:    2 RSQFKDEHPFEKRQAEAARIAQRFKDRVPVICEKVENSDIPEIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_PICPA (Autophagy-related protein 8 OS=Pichia pastoris GN=ATG8 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 5.559e-31
Identity = 40/54 (74.07%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP DLTVGQFVYV+RKRIK+ +EKAIFIF+ +ILPPTAA+MS +YEE+K
Sbjct:   46 KRKYLVPCDLTVGQFVYVIRKRIKIPSEKAIFIFIDDILPPTAALMSKVYEEHK 99

HSP 2 Score: 65.855 bits (159), Expect = 5.559e-31
Identity = 29/46 (63.04%), Postives = 38/46 (82.61%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+R+AE+ RIR K+ DRIPVI EK E+SDIP++DK+
Sbjct:    2 RSQFKDEHPFEKRKAEAMRIRYKFADRIPVICEKVEKSDIPEVDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_SCHPO (Autophagy-related protein 8 OS=Schizosaccharomyces pombe GN=atg8 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.231e-30
Identity = 44/54 (81.48%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KKYLVP+DLTVGQFVYV+RKRIKLS EKAIFIF+  ILPPTAA+MS IYEE+K
Sbjct:   46 KKKYLVPSDLTVGQFVYVIRKRIKLSPEKAIFIFIDEILPPTAALMSTIYEEHK 99

HSP 2 Score: 60.8474 bits (146), Expect = 1.231e-30
Identity = 29/46 (63.04%), Postives = 35/46 (76.09%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK +   E+R+ ES RIREKYPDRIPVI EK ++SDI  IDKK
Sbjct:    2 RSQFKDDFSFEKRKTESQRIREKYPDRIPVICEKVDKSDIAAIDKK 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_VANPO (Autophagy-related protein 8 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) GN=ATG8 PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.235e-30
Identity = 40/54 (74.07%), Postives = 49/54 (90.74%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP+DLTVGQFVYV+RKRI L  EKAIFIFV + LPPTAA+MSA+Y+E+K
Sbjct:   46 KRKYLVPSDLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAVYQEHK 99

HSP 2 Score: 67.0106 bits (162), Expect = 1.235e-30
Identity = 31/46 (67.39%), Postives = 39/46 (84.78%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            KSSFK E+P E+R+ ES RI EK+ +RIPVI EKAE+SDIP+IDK+
Sbjct:    2 KSSFKSEYPFEKRKTESERITEKFQNRIPVICEKAEKSDIPEIDKR 47
The following BLAST results are available for this feature:
BLAST of DR403235 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 72
Match NameE-valueIdentityDescription
ATG8_SCLS12.335e-3281.48Autophagy-related protein 8 OS=Sclerotinia sclerot... [more]
ATG8_CHAGB2.340e-3281.48Autophagy-related protein 8 OS=Chaetomium globosum... [more]
ATG8_EMENI2.347e-3283.33Autophagy-related protein 8 OS=Emericella nidulans... [more]
ATG8_BOTFB3.041e-3281.48Autophagy-related protein 8 OS=Botryotinia fuckeli... [more]
ATG8_MAGGR1.139e-3181.48Autophagy-related protein 8 OS=Magnaporthe grisea ... [more]
ATG8_LODEL1.915e-3177.78Autophagy-related protein 8 OS=Lodderomyces elongi... [more]
ATG8_CANAL1.921e-3177.78Autophagy-related protein 8 OS=Candida albicans GN... [more]
ATG8_PICPA5.559e-3174.07Autophagy-related protein 8 OS=Pichia pastoris GN=... [more]
ATG8_SCHPO1.231e-3081.48Autophagy-related protein 8 OS=Schizosaccharomyces... [more]
ATG8_VANPO1.235e-3074.07Autophagy-related protein 8 OS=Vanderwaltozyma pol... [more]

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Properties
Property NameValue
Genbank descriptionCSAF-PNP1244E11 Flavedo Mature Citrus sinensis cDNA clone CSAF-PNP1244E11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR403235 ID=DR403235; Name=DR403235; organism=Citrus sinensis; type=EST; length=388bp
GGGGAGTCTTTTTATTTATTTATTTATTTTAATCCGTCGATTCATATTCA
TTTTCCCCTTGGAAGCAGTAGAAAGAACAAGGAGAAGCTATGGCCAAGAG
CTCCTTCAAGCTTGAACACCCACTCGAAAGGAGGCAGGCTGAATCTGCCC
GTATCAGGGAGAAGTATCCTGATAGAATACCAGTGATTGTGGAGAAGGCT
GAAAGGAGTGACATACCTGATATTGACAAAAAAAATACCTGGTTCCTGCT
GATCTGACTGTTGGACAATTTGTTTATGTGGTCCGGAAGAGGATAAAGCT
CAGTGCTGAGAAGGCCATATTCATATTTGTCAAGAACATTTTGCCACCCA
CTGCTGCAATGATGTCTGCAATTTATGAGGAAAACAAA
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