CX308586

Overview
NameCX308586
Unique NameCX308586
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length447
Libraries
Library NameType
AbsLeaSub1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig735 contig Ccv1_Contig735:257..703. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: HBP1_CASGL (Hemoglobin-1 OS=Casuarina glauca PE=1 SV=2)

HSP 1 Score: 113.235 bits (282), Expect = 4.951e-25
Identity = 59/121 (48.76%), Postives = 78/121 (64.46%), Query Frame = -1
Query:    1 AFTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKEA 363
            A TE+QEAL+ +SW V+K+N     L+ F  I E AP ++ +FSFL DSN   E N KLK HA  +F   CESA +LR+ G      +TLK+LG++H K  + + HFEV K +LL TIKEA
Sbjct:    2 ALTEKQEALLKQSWEVLKQNIPAHSLRLFALIIEAAPESKYVFSFLKDSNEIPENNPKLKAHAAVIFKTICESATELRQKGHAVWDNNTLKRLGSIHLKNKITDPHFEVMKGALLGTIKEA 122          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB_CANLI (Leghemoglobin OS=Canavalia lineata PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.543e-21
Identity = 58/121 (47.93%), Postives = 76/121 (62.81%), Query Frame = -1
Query:    1 AFTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKEA 363
            AF+E+QE+LV  SW   K+N       F+  I E AP+AQ +FSFL +   P   N KLK HA  VF MT +SAVQLR  G+V + + T   LG+VH + GV++ HF V K +LL+T KEA
Sbjct:    3 AFSEKQESLVKSSWEAFKQNVPHHSAVFYTLILEKAPAAQNMFSFLSNGVDP--NNPKLKAHAEKVFKMTVDSAVQLRAKGEVVLADPT---LGSVHVQKGVLDPHFLVVKEALLKTFKEA 118          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB3_PEA (Leghemoglobin Lb120-1 OS=Pisum sativum PE=2 SV=3)

HSP 1 Score: 98.2117 bits (243), Expect = 1.648e-20
Identity = 58/120 (48.33%), Postives = 80/120 (66.67%), Query Frame = -1
Query:    1 FTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKEA 360
            FTE+QEALV  SW + K+N     + F+  I + AP+A+ +FSFL DS   +  + KL+ HA  VF M  +SA+QLR +G+V V ++TL   GA+H + GVV+ HF V K +LLETIKEA
Sbjct:    3 FTEKQEALVNSSWELFKQNPS-YSVLFYTIILKKAPAAKGMFSFLKDS-AEVVDSPKLQAHAEKVFGMVHDSAIQLRASGEVVVGDATL---GAIHIQKGVVDPHFVVVKEALLETIKEA 117          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB2_LUPLU (Leghemoglobin-2 OS=Lupinus luteus PE=1 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 1.648e-20
Identity = 57/121 (47.11%), Postives = 77/121 (63.64%), Query Frame = -1
Query:    4 AFTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFL-GDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKE 363
            A TE Q ALV  SW     N  +   +FF+ + EIAP+A+ LFSFL G S VP + N +L+ HA  VF +  E+A+QL+  G V V ++TLK LG+VH   GV + HF V K ++L+TIKE
Sbjct:    3 ALTESQAALVKSSWEEFNANIPKHTHRFFILVLEIAPAAKDLFSFLKGTSEVP-QNNPELQAHAGKVFKLVYEAAIQLQVTG-VVVTDATLKNLGSVHVSKGVADAHFPVVKEAILKTIKE 121          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB2_MEDTR (Leghemoglobin 2 OS=Medicago truncatula GN=LB2 PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.811e-20
Identity = 58/119 (48.74%), Postives = 80/119 (67.23%), Query Frame = -1
Query:    4 FTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKE 360
            FTE+QEALV  SW + K+N G   L F+  I E AP+A+ +FSFL D+   ++ + KL++HA  VF M  +SAVQLR  G V + ++TL   GA+H + GVV+ HF V K +LL+TIKE
Sbjct:    3 FTEKQEALVNSSWELFKQNPGNSVL-FYTIILEKAPAAKGMFSFLKDT-AGVQDSPKLQSHAEKVFGMVRDSAVQLRATGGVVLGDATL---GAIHIQKGVVDPHFVVVKEALLKTIKE 116          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB6_PEA (Leghemoglobin Lb120-34 OS=Pisum sativum PE=2 SV=3)

HSP 1 Score: 97.0561 bits (240), Expect = 3.671e-20
Identity = 57/120 (47.50%), Postives = 80/120 (66.67%), Query Frame = -1
Query:    1 FTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKEA 360
            FTE+QEALV  SW + K+N     + F+  I + AP+A+ +FSFL DS   +  + KL+ HA  VF M  +SA+QLR +G+V + ++TL   GA+H + GVV+ HF V K +LLETIKEA
Sbjct:    3 FTEKQEALVNSSWELFKQNPS-YSVLFYTIILKKAPAAKGMFSFLKDS-AEVVDSPKLQAHAEKVFGMVHDSAIQLRASGEVVLGDATL---GAIHIQKGVVDPHFVVVKEALLETIKEA 117          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB5_PEA (Leghemoglobin Lb120-29 OS=Pisum sativum PE=2 SV=3)

HSP 1 Score: 95.9005 bits (237), Expect = 8.179e-20
Identity = 57/120 (47.50%), Postives = 80/120 (66.67%), Query Frame = -1
Query:    1 FTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKEA 360
            FT++QEALV  SW + K+N G   + F+  I + AP+ + +FSFL DS   +  + KL+ HA  VF M  +SAVQLR +G+V + ++TL   GA+H + GVV+ HF V K +LLETIKEA
Sbjct:    3 FTDKQEALVNSSWELFKQNPG-YSVLFYNIILKKAPATKGMFSFLKDS-AGVVDSPKLQAHAEKVFGMVHDSAVQLRVSGEVVLGDATL---GAIHIQKGVVDSHFVVVKEALLETIKEA 117          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB4_MEDSA (Leghemoglobin OS=Medicago sativa GN=LB3 PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 8.179e-20
Identity = 55/120 (45.83%), Postives = 78/120 (65.00%), Query Frame = -1
Query:    1 FTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKEA 360
            FT +QEALV  SW   K+N     + F+  I E AP+A+ +FSFL DS   ++ + +L+ HA  VF M  +SAVQLR  G+V + ++T   LG++H + GVV+ HF V K +LL+TIKEA
Sbjct:    3 FTADQEALVNSSWESFKQNLPGYSVFFYTTILEKAPAAKGMFSFLKDS-AGVQDSPQLQAHAEKVFGMVRDSAVQLRATGEVVLGDAT---LGSIHIQKGVVDPHFVVVKEALLKTIKEA 118          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB1_MEDSA (Leghemoglobin-1 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.068e-19
Identity = 54/121 (44.63%), Postives = 79/121 (65.29%), Query Frame = -1
Query:    1 AFTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKEA 363
            +FT++QEALV  SW   K+N     + F+  + E AP+A+ LFSFL +S   ++ + +L+ HA  VF +  +SAVQLR  G V + ++T   LGA+H + GVV+ HF V K +LL+TIKEA
Sbjct:    2 SFTDKQEALVNSSWEAFKQNLPRYSVFFYTVVLEKAPAAKGLFSFLKNS-AEVQDSPQLQAHAEKVFGLVRDSAVQLRATGGVVLGDAT---LGAIHVRKGVVDPHFVVVKEALLKTIKEA 118          
BLAST of CX308586 vs. ExPASy Swiss-Prot
Match: LGB3_MEDSA (Leghemoglobin-3 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.395e-19
Identity = 57/119 (47.90%), Postives = 78/119 (65.55%), Query Frame = -1
Query:    4 FTEEQEALVVKSWNVMKKNAGELGLKFFLRIFEIAPSAQKLFSFLGDSNVPLEQNQKLKTHAMSVFVMTCESAVQLRKAGKVTVRESTLKKLGAVHFKYGVVNEHFEVTKFSLLETIKE 360
            FT++QEALV  SW   K+N G   L F+  I E AP+A+ +FSFL DS   ++ + KL++HA  VF M  +SA QLR  G V + ++TL   GA+H + GVV+ HF V K +LL+TIKE
Sbjct:    3 FTDKQEALVNSSWESFKQNPGNSVL-FYTIILEKAPAAKGMFSFLKDS-AGVQDSPKLQSHAEKVFGMVRDSAAQLRATGGVVLGDATL---GAIHIQKGVVDPHFAVVKEALLKTIKE 116          
The following BLAST results are available for this feature:
BLAST of CX308586 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 50
Match NameE-valueIdentityDescription
HBP1_CASGL4.951e-2548.76Hemoglobin-1 OS=Casuarina glauca PE=1 SV=2[more]
LGB_CANLI2.543e-2147.93Leghemoglobin OS=Canavalia lineata PE=2 SV=1[more]
LGB3_PEA1.648e-2048.33Leghemoglobin Lb120-1 OS=Pisum sativum PE=2 SV=3[more]
LGB2_LUPLU1.648e-2047.11Leghemoglobin-2 OS=Lupinus luteus PE=1 SV=2[more]
LGB2_MEDTR2.811e-2048.74Leghemoglobin 2 OS=Medicago truncatula GN=LB2 PE=2... [more]
LGB6_PEA3.671e-2047.50Leghemoglobin Lb120-34 OS=Pisum sativum PE=2 SV=3[more]
LGB5_PEA8.179e-2047.50Leghemoglobin Lb120-29 OS=Pisum sativum PE=2 SV=3[more]
LGB4_MEDSA8.179e-2045.83Leghemoglobin OS=Medicago sativa GN=LB3 PE=2 SV=1[more]
LGB1_MEDSA1.068e-1944.63Leghemoglobin-1 OS=Medicago sativa PE=2 SV=1[more]
LGB3_MEDSA1.395e-1947.90Leghemoglobin-3 OS=Medicago sativa PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionC21001F01Rv AbsLeaSub1 Citrus clementina cDNA clone C21001F01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX308586 ID=CX308586; Name=CX308586; organism=Citrus clementina; type=EST; length=447bp
TGCCTCTTTTATGGTTTCCAGTAGTGAAAATTTTGTGACCTCAAAATGCT
CATTAACAACACCATATTTGAAGTGGACAGCTCCTAATTTTTTTAGAGTT
GATTCTCTTACTGTCACTTTGCCTGCTTTCCTCAGTTGCACTGCTGATTC
ACACGTCATAACAAAAACAGACATGGCATGGGTCTTGAGCTTCTGGTTCT
GCTCTAGAGGCACATTTGAGTCTCCCAAGAATGAGAACAGCTTTTGGGCT
GATGGTGCAATCTCAAAGATCCTCAAGAAAAATTTAAGACCCAATTCTCC
AGCATTCTTCTTCATTACATTCCATGACTTAACAACTAAAGCTTCTTGCT
CTTCAGTAAAGGCTCTACCTTCGTTAATGTTGGCCATTGATTTCTATTTG
GACACCTGAGGATTGAATGTGATACACAAAATGTTGTTGAATCCCCG
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