Genetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliata (L.) Raf. and mapping of citrus tristeza virus resistance gene

Publication Overview
TitleGenetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliata (L.) Raf. and mapping of citrus tristeza virus resistance gene
AuthorsCristofani M, Machado M, Grattapaglia D
TypeJournal Article
Journal NameEuphytica
Volume109
Issue1
Year1999
Page(s)25-32
CitationCristofani M, Machado M, Grattapaglia D. Genetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliata (L.) Raf. and mapping of citrus tristeza virus resistance gene. Euphytica : Netherlands journal of plant breeding. 1999; 109(1):25-32.

Abstract

A linkage map for Citrus sunki Hort. ex. Tan. and Poncirus trifoliate (L.) Raf cv. Rubidoux has been developed exploiting the pseudo-testcross mapping strategy and RAPD markers. From controlled crosses 314 F1 zygotic progeny individuals were obtained. Two separate data sets were generated, one for each parent. Preliminary linkage analysis was carried out with a phase-unknown backcross model at a LOD > 4.0 and max. Theta = 0.30. A total of 203 arbitrary 10-mer primers and 5 arbitrary 8-mer primers were screened for segregating RAPD markers. Pseudo-testcrosss mating configurations were displayed by 78 primers where the marker was present in one parent, absent in the other and segregating in the progeny. These 78 primers were run on 80 individuals of the progeny amplifying a total of 169 RAPD markers. Linkage analysis revealed that 125 of these RAPD loci fell into 18 linkage groups (10 groups for ‘Sunki’ and 8 groups for ‘Rubidoux’) while 44 loci remained unassigned to any linkage group. The total length of the maps was 732.32 cM for ‘Sunki’ and 866.88 cM for ‘Rubidoux’ with the distance between markers ranging from 0 to 43.8 cM. Bulked segregant analysis (BSA) was used to identify potential resistance-associated RAPD fragment markers. Two RAPD fragments were found flanking the CTV gene (OPAV12_470 - 17.8 cM and OPN15_1630 - 23.1 cM).
Features
This publication contains information about 71 features:
Feature NameUniquenameType
M12M12genetic_marker
AV03AV03genetic_marker
C06C06genetic_marker
R07R07genetic_marker
A01A01genetic_marker
E15E15genetic_marker
M09M09genetic_marker
C15C15genetic_marker
M16M16genetic_marker
M20M20genetic_marker
AV05AV05genetic_marker
Q14Q14genetic_marker
M02M02genetic_marker
N09N09genetic_marker
M05M05genetic_marker
R19R19genetic_marker
N12N12genetic_marker
CtvCtvgenetic_marker
AVAVgenetic_marker
W18W18genetic_marker
AV11AV11genetic_marker
E07E07genetic_marker
E16E16genetic_marker
E20E20genetic_marker
K07K07genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-Sunki/Rubidoux-Sunki-F1-1999
Citrus-Sunki/Rubidoux-Rubidoux-F1-1999
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
Citrus sunkiCitrus sunkiaccession
RubidouxRubidouxaccession
Citrus-Sunki/Rubidoux-Sunki-F1Citrus-Sunki/Rubidoux-Sunki-F1population
Citrus-Sunki/Rubidoux-Rubidoux-F1Citrus-Sunki/Rubidoux-Rubidoux-F1population
Properties
Additional details for this publication include:
Property NameValue
Language Abbreng
Publication Date1999
Publication TypeJournal Article
KeywordsPoncirus trifoliata, Citrus tristeza virus, disease resistance, genetic resistance, genes, chromosome mapping, genetic markers, progeny, linkage (genetics), random amplified polymorphic DNA technique, segregation, gene location, citrus sunki