Extension of the linkage map in Citrus using random amplified polymorphic DNA (RADP) markers and RFLP mapping of cold-acclimation-responsive loci

Publication Overview
TitleExtension of the linkage map in Citrus using random amplified polymorphic DNA (RADP) markers and RFLP mapping of cold-acclimation-responsive loci
AuthorsCai Q, Guy C, Moore G
TypeJournal Article
Journal NameTheoretical and applied genetics
Volume89
Issue5
Year1994
Page(s)606-614
CitationCai Q, Guy C, Moore G. Extension of the linkage map in Citrus using random amplified polymorphic DNA (RADP) markers and RFLP mapping of cold-acclimation-responsive loci. Theoretical and applied genetics. 1994 Nov; 89(5):606-614.

Abstract

Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. X [Citrus grandis (L.) Osb. X Poncirus trifoliata (L.) Raf.]. A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70-80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning we have mapped three loci detected by a cold-acclimation-responsive cDNA.
Features
This publication contains information about 124 features:
Feature NameUniquenameType
Got1Got1genetic_marker
IdhIdhgenetic_marker
Mdh2Mdh2genetic_marker
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OPA01OPA01genetic_marker
OPA02OPA02genetic_marker
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OPE08OPE08genetic_marker
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Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Citrus-Pomeroy/2*ThongDee-BC1-1994
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
ThongDeeThongDeeaccession
PomeroyPomeroyaccession
Citrus-Pomeroy/2*ThongDee-BC1Citrus-Pomeroy/2*ThongDee-BC1population
Properties
Additional details for this publication include:
Property NameValue
Language Abbreng
Publication Date1994 Nov
Publication TypeJournal Article
Journal AbbreviationTheor. appl. genet.
KeywordsCitrus maxima, Poncirus trifoliata, intergeneric hybridization, genetic markers, restriction fragment length polymorphism, loci, cold, acclimation, chromosome mapping, linkage groups