QTL mapping of male sterility and transmission pattern in progeny of Satsuma mandarin.

Publication Overview
TitleQTL mapping of male sterility and transmission pattern in progeny of Satsuma mandarin.
AuthorsGoto S, Yoshioka T, Ohta S, Kita M, Hamada H, Shimizu T
TypeJournal Article
Journal NamePloS one
Volume13
Issue7
Year2018
Page(s)e0200844
CitationGoto S, Yoshioka T, Ohta S, Kita M, Hamada H, Shimizu T. QTL mapping of male sterility and transmission pattern in progeny of Satsuma mandarin.. PloS one. 2018; 13(7):e0200844.

Abstract

Seedlessness is one of the important traits in citrus breeding. Male sterility derived from Satsuma mandarin (Citrus unshiu) has been used in Japanese citrus breeding programs to obtain seedless cultivars. The efficiency of seedless cultivar breeding would be improved by developing a selection marker linked to seedlessness. In this study, we performed QTL mapping in 'Okitsu No. 46' × 'Okitsu No. 56' (O46-O56) crosses for the number of pollen grains per anther (NPG) and apparent pollen fertility (APF), two traits used as an index of male sterility, and detected a candidate QTL for NPG (MS-P1) on linkage group 8 with a significant LOD score (7.31) and 47% of variance explained. The QTL for APF (MS-F1) was detected on linkage group 6 with a significant LOD score (5.71) and 63.6% of variance explained. The role of both MS-P1 in reducing NPG and MS-F1 in decreasing APF were confirmed with the 'Okitsu No.46' × 'Kara' (O46-K) cross. Pedigree analysis inferred that both MS-P1 and MS-F1 in 'Okitsu No. 46' were derived from kunenbo (Citrus nobilis) through hassaku (C. hassaku) and 'Sweet Spring'. Cytoplasm analysis revealed that both male-sterile 'Sweet Spring' and 'Okitsu No. 46' have cytoplasm derived from Kishu (C. kinokuni hort. ex Tanaka), but the cytoplasm of male-sterile kunenbo and hassaku were derived from other varieties rather than Kishu. These results suggest that MS-P1 and MS-F1 primarily reduce the NPG and decrease APF, but their expression requires a cytoplasm derived from Kishu. These findings will improve our understanding of the molecular mechanism of male sterility in citrus and help to develop a DNA marker for seedless breeding in citrus.

Featuremaps
This publication contains information about 1 maps:
Map Name
Citrus-Okitsu46/Okitsu56-F1
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
Okitsu46/Okitsu56-F1Okitsu46/Okitsu56-F1population
Projects
This publication contains information about 1 projects:
Project NameDescription
Citrus-Male_sterility-Goto-2018
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1932-6203
eISSN1932-6203
Publication Date2018
Journal AbbreviationPLoS ONE
DOI10.1371/journal.pone.0200844
Elocation10.1371/journal.pone.0200844
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
Cross References
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DatabaseAccession
PMID: PubMedPMID:30016346