Ectopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress

Publication Overview
TitleEctopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress
AuthorsCaruso P, Baldoni E, Mattana M, Pietro Paolo D, Genga A, Coraggio I, Russo G, Picchi V, Reforgiato Recupero G, Locatelli F
TypeJournal Article
Journal Name"Plant cell, tissue, and organ culture"
Volume109
Issue2
Year2012
Page(s)327-339
CitationCaruso P, Baldoni E, Mattana M, Pietro Paolo D, Genga A, Coraggio I, Russo G, Picchi V, Reforgiato Recupero G, Locatelli F. Ectopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress. Plant cell, tissue, and organ culture. 2012; 109(2):327-339.

Abstract

Oxygen deficit, which occurs in flooded or poorly drained soils, can limit plant growth and development. Low-oxygen environmental conditions also limit the distribution of many woody plants, such as citrus trees, which are considered flood-sensitive crops, although tolerance to this stress varies among genotypes and rootstocks. In this study, the rice transcription factor Mybleu was inserted into the pGA470 plant cloning vector and transferred into the epicotyl explants of the Carrizo citrange rootstock (Citrus sinensis × Poncirus trifoliata) using Agrobacterium tumefaciens-mediated transformation. The transgenic lines were confirmed for the presence and expression of the transgene, and physiological, biochemical and molecular parameters were evaluated for adaptation to hypoxic and anoxic stress conditions. The ectopic expression of Mybleu increased tolerance to oxygen deprivation in the transgenic lines, contributing to increased viability under this stress condition. This improved tolerance correlates with, and may depend on, the induction of genes and the activation of enzymes from various fermentation and carbohydrate metabolic pathways, antioxidant systems and nonsymbiotic haemoglobin-nitric oxide homeostasis mechanisms. Together, our data suggest a key role for Mybleu in coordinating the multifaceted plant response to low oxygen stress and the conservation of Mybleu-regulated pathways among species.
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
Carrizo CitrangeCarrizo Citrangebreeding_research_material
Features
This publication contains information about 15 features:
Feature NameUniquenameType
HB1HB1genetic_marker
PDCPDCgenetic_marker
ADHADHgenetic_marker
SUS1SUS1genetic_marker
SUSASUSAgenetic_marker
CATCATgenetic_marker
APX2APX2genetic_marker
SODSODgenetic_marker
GRGRgenetic_marker
18S18Sgenetic_marker
EF-1aEF-1agenetic_marker
MGBMGBgenetic_marker
MybleuMybleugenetic_marker
NOS-RNOS-Rgenetic_marker
P35S-FP35S-Fgenetic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Model[electronic resource].
Language Abbreng
Published Location|||
URLhttp://dx.doi.org/10.1007/s11240-011-0098-1
Publication Date2012
KeywordsAgrobacterium, Citroncirus webberi, Citrus sinensis, Poncirus trifoliata, anaerobic conditions, antioxidants, biochemical pathways, carbohydrates, cloning (plants), crops, enzyme activation, fermentation, gene activation, gene expression, gene induction, genetic vectors, genotype, oxides, oxygen, plant development, plant response, poorly drained soils, rice, rootstocks, stress tolerance, transcription factors, transgenic plants, trees, viability, woody plants
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4676514