Development of Species-Specific InDel Markers in Citrus

Publication Overview
TitleDevelopment of Species-Specific InDel Markers in Citrus
AuthorsFang Q, Wang L, Yu H, Huang Y, Jiang X, Deng X, Xu Q
TypeJournal Article
Page(s)653 - 662
CitationFang Q, Wang L, Yu H, Huang Y, Jiang X, Deng X, Xu Q. Development of Species-Specific InDel Markers in Citrus. Plant Mol Biol Rep. 2018; 36:653-662.


Citrus taxonomy is complex owing to the existence of a wide range of species: Poncirus is used mainly for rootstock; Fortunella produces small fruit and edible pericarp; and Citrus comprises the most widespread fruit crop species worldwide. Rapidly increasing genome resources from different citrus species facilitate the development of convenient and genome-wide molecular markers that can be applied to both inter- and intra-species analyses. In this study, by comparing the genome sequences of four citrus species, a set of 1958 InDels were identified and 453 candidate InDels were converted into PCR-based markers. Among these candidate InDels, 268 (65%) exhibited length polymorphisms from 30 bp to 200 bp when applied to seven species from the genera Poncirus, Fortunella and Citrus. Seven InDel markers exhibited high intraspecific polymorphisms in a natural pummelo population. The results showed that the InDel markers are effective for both inter- and intra-specific variation and identification analyses. These InDel markers are expected to be applied to germplasm identification, phylogenetic analysis, genetic diversity evaluation and marker-assisted breeding in citrus.
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Series NamePlant Molecular Biology Reporter