FC875486

Overview
NameFC875486
Unique NameFC875486
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length702
Libraries
Library NameType
CTVClemen1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig15417 contig Ccv1_Contig15417:157..858. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_BETVU (Elongation factor 2 OS=Beta vulgaris PE=2 SV=1)

HSP 1 Score: 449.129 bits (1154), Expect = 1.032e-125
Identity = 217/230 (94.35%), Postives = 224/230 (97.39%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVL++S RTVMSKSPNKHNRLYMEARP+EEGLAEAID+GRIGPRDDPK RSKIL+EE+GWDKDLAKKIWCFGPET GPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLH DAIHRGGGQ+IPTARRV YASQLTAKPRLLEPVYLVEIQAPE ALGGIYSVLNQKRGHVFEEMQRPG
Sbjct:  534 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLDRSVRTVMSKSPNKHNRLYMEARPMEEGLAEAIDEGRIGPRDDPKNRSKILAEEYGWDKDLAKKIWCFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQIIPTARRVFYASQLTAKPRLLEPVYLVEIQAPENALGGIYSVLNQKRGHVFEEMQRPG 763
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_CHLKE (Elongation factor 2 OS=Chlorella kessleri PE=2 SV=1)

HSP 1 Score: 400.979 bits (1029), Expect = 3.223e-111
Identity = 195/230 (84.78%), Postives = 212/230 (92.17%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDLQDDFMGGAEI  S+PVVSFRETV+  S   VMSKSPNKHNRLYM+ARP+E+GLAEAID+G+IGPRDDPKVRSKILSEEFGWDK+LAKKI  FGP+T GPNMV D+ KGVQYLNEIKDSVVA FQWASKEG LAEENMRGI FEVCDVVLHADAIHRGGGQ+IPTARR +YA+QLTA+PRLLEPVYLVEIQ PEQA+GG+YSVLNQKRG VFEE+QRPG
Sbjct:  536 GELHLEICLKDLQDDFMGGAEIRVSEPVVSFRETVIGTSDHVVMSKSPNKHNRLYMQARPMEDGLAEAIDEGKIGPRDDPKVRSKILSEEFGWDKELAKKILAFGPDTTGPNMVTDITKGVQYLNEIKDSVVAAFQWASKEGVLAEENMRGIVFEVCDVVLHADAIHRGGGQIIPTARRSMYAAQLTAQPRLLEPVYLVEIQCPEQAMGGVYSVLNQKRGMVFEELQRPG 765
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_RAT (Elongation factor 2 OS=Rattus norvegicus GN=Eef2 PE=1 SV=4)

HSP 1 Score: 323.168 bits (827), Expect = 8.540e-88
Identity = 158/230 (68.70%), Postives = 187/230 (81.30%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKARARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAG 778
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_MOUSE (Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 SV=2)

HSP 1 Score: 323.168 bits (827), Expect = 8.540e-88
Identity = 158/230 (68.70%), Postives = 187/230 (81.30%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKARARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAG 778
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_MESAU (Elongation factor 2 OS=Mesocricetus auratus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 323.168 bits (827), Expect = 8.540e-88
Identity = 158/230 (68.70%), Postives = 187/230 (81.30%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKARARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAG 778
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_PONAB (Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 SV=3)

HSP 1 Score: 322.398 bits (825), Expect = 1.457e-87
Identity = 158/230 (68.70%), Postives = 187/230 (81.30%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAG 778
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_HUMAN (Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4)

HSP 1 Score: 322.398 bits (825), Expect = 1.457e-87
Identity = 158/230 (68.70%), Postives = 187/230 (81.30%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAG 778
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_CALJA (Elongation factor 2 OS=Callithrix jacchus GN=EEF2 PE=2 SV=1)

HSP 1 Score: 322.398 bits (825), Expect = 1.457e-87
Identity = 158/230 (68.70%), Postives = 187/230 (81.30%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAG 778
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_BOVIN (Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 322.398 bits (825), Expect = 1.457e-87
Identity = 158/230 (68.70%), Postives = 187/230 (81.30%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEETQVAG 778
[Close]
BLAST of FC875486 vs. ExPASy Swiss-Prot
Match: EF2_CRIGR (Elongation factor 2 OS=Cricetulus griseus GN=EEF2 PE=3 SV=3)

HSP 1 Score: 320.087 bits (819), Expect = 7.230e-87
Identity = 157/230 (68.26%), Postives = 186/230 (80.87%), Query Frame = 2 
Query:    2 GELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYLVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPG 691
            GELHLEICLKDL++D      I KSDPVVS+RETV E+S    +SKSPNKHNRLYM+ARP  +GLAE ID G +  R + K R++ L+E++ WD   A+KIWCFGP+  GPN++ D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHR GGQ+IPTARR +YAS LTA+PRL+EP+YLVEIQ PEQ +GGIY VLN+KRGHVFEE Q  G
Sbjct:  550 GELHLEICLKDLEEDH-ACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKARARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRRGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAG 778
The following BLAST results are available for this feature:
BLAST of FC875486 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 97
Match NameE-valueIdentityDescription
EF2_BETVU1.032e-12594.35Elongation factor 2 OS=Beta vulgaris PE=2 SV=1[more]
EF2_CHLKE3.223e-11184.78Elongation factor 2 OS=Chlorella kessleri PE=2 SV=... [more]
EF2_RAT8.540e-8868.70Elongation factor 2 OS=Rattus norvegicus GN=Eef2 P... [more]
EF2_MOUSE8.540e-8868.70Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 S... [more]
EF2_MESAU8.540e-8868.70Elongation factor 2 OS=Mesocricetus auratus GN=EEF... [more]
EF2_PONAB1.457e-8768.70Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 S... [more]
EF2_HUMAN1.457e-8768.70Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 S... [more]
EF2_CALJA1.457e-8768.70Elongation factor 2 OS=Callithrix jacchus GN=EEF2 ... [more]
EF2_BOVIN1.457e-8768.70Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=... [more]
EF2_CRIGR7.230e-8768.26Elongation factor 2 OS=Cricetulus griseus GN=EEF2 ... [more]

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Properties
Property NameValue
Genbank descriptionC31404E02EF CTVClemen1 Citrus clementina cDNA clone C31404E02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC875486 ID=FC875486; Name=FC875486; organism=Citrus clementina; type=EST; length=702bp
AGGAGAACTCCATCTTGAGATCTGTTTGAAGGATTTGCAGGATGATTTCA
TGGGTGGTGCTGAGATTATCAAATCTGATCCTGTTGTGTCCTTCCGTGAA
ACTGTCCTTGAGAAGTCCTGCCGTACTGTTATGAGCAAGTCTCCAAACAA
ACACAATCGTCTCTATATGGAAGCCCGACCATTGGAGGAAGGTCTTGCCG
AGGCAATTGATGATGGTCGTATTGGTCCAAGGGATGATCCTAAGGTTCGT
TCTAAGATTCTGTCTGAGGAATTTGGTTGGGACAAGGATCTTGCCAAGAA
AATCTGGTGTTTTGGTCCTGAGACCATTGGTCCTAACATGGTTGTGGATA
TGTGCAAGGGAGTTCAGTACTTGAATGAAATTAAGGATTCAGTTGTTGCT
GGGTTCCAGTGGGCATCAAAGGAAGGTGCATTGGCTGAAGAAAACATGAG
GGGCATTTGCTTTGAAGTTTGTGATGTGGTTCTTCATGCCGATGCTATCC
ACAGAGGTGGTGGCCAGGTTATTCCAACTGCTAGAAGGGTCATTTATGCA
TCCCAGCTTACAGCCAAACCAAGGCTTCTTGAGCCTGTCTACTTAGTGGA
AATCCAAGCTCCTGAACAGGCACTTGGTGGTATTTACAGTGTTCTTAACC
AGAAACGTGGTCACGTTTTTGAGGAGATGCAGAGACCTGGTCCCCACTTT
AT
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