FC920550

Overview
NameFC920550
Unique NameFC920550
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length726
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig14122 contig Ccv1_Contig14122:32..758. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 352.443 bits (903), Expect = 1.385e-96
Identity = 168/205 (81.95%), Postives = 185/205 (90.24%), Query Frame = 2 
Query:  104 WPKKDKRRLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGG-NVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEYTR 715
            WPKKDK+RLLHAVYRVGDLDRTIK YTECFGMKLLRKRDVPEEKY+NAFLGFGPE ++F +ELTYNYGV  YDIG GFGHFAIATEDVYKL E I++     +TREPGPVKGG+T IAF +DPDGY+FELIQRGPTPEPLCQVMLRVGDL RSIKFYEKALGMKLLR  D P+YKYT+AMLGYA+ED+TTV+E TYNYGVTEYT+
Sbjct:   17 WPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTK 221

HSP 2 Score: 125.946 bits (315), Expect = 2.109e-28
Identity = 64/126 (50.79%), Postives = 80/126 (63.49%), Query Frame = 2 
Query:  128 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENI----RAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELI 493
            L   + RVGDLDR+IKFY +  GMKLLRK+DVP+ KY+ A LG+  E    V+ELTYNYGVT Y  G  +   AI TEDVYK  E +    +  GG + R+PGP+ G  T IA   DPDG+   L+
Sbjct:  156 LCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLV 281
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 352.443 bits (903), Expect = 1.385e-96
Identity = 167/204 (81.86%), Postives = 187/204 (91.67%), Query Frame = 2 
Query:  104 WPKKDKRRLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEYTR 715
            WPKKDKRR LH VYRVGDLDRTI+FYTECFGMK+LRKRDVPEEKYSNAFLGFGPE S+FVVELTYNYGV+SYDIGTGFGHFAI+T+DV K+VE +RAKGGNVTREPGPVKGG + IAFVKDPDGY FELIQRGPTPEPLCQVMLRVGDL R++KF EKALGM+LLR +++PEY  T+ M+GYAEE ++ VLE TYNYGVTEYT+
Sbjct:   10 WPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTK 212

HSP 2 Score: 99.7525 bits (247), Expect = 1.619e-20
Identity = 53/121 (43.80%), Postives = 70/121 (57.85%), Query Frame = 2 
Query:  128 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIR----AKGGNVTREPGPVKGGTTHIAFVKDPDGY 478
            L   + RVGDLDR +KF  +  GM+LLR+ + PE   +   +G+  E    V+ELTYNYGVT Y  G  +   AI T+DVYK  E ++      GG +TRE GP+ G  T I    DPDG+
Sbjct:  148 LCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGW 267
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 168.703 bits (426), Expect = 2.837e-41
Identity = 78/124 (62.90%), Postives = 96/124 (77.42%), Query Frame = 2 
Query:  125 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            R+LH + RVGDLD++IKFYTE  GM+LLR  +  E +Y+ AF+G+G E    V+ELTYN+G T YD+GT FGH AI  +D+Y   + I+A GGNVTRE GPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 81.6481 bits (200), Expect = 4.561e-15
Identity = 37/64 (57.81%), Postives = 48/64 (75.00%), Query Frame = 2 
Query:  518 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEY 709
            +   MLRVGDL +SIKFY + +GM+LLRT +  EY+YTLA +GY +E Q  V+E TYN+G TEY
Sbjct:    6 ILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEY 69
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 164.851 bits (416), Expect = 4.097e-40
Identity = 76/124 (61.29%), Postives = 94/124 (75.81%), Query Frame = 2 
Query:  125 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            R+LH + RVGDLD++I+FYT+  GM LLRK +  E KY+ AFLG+G E    V+ELTYN+GV  Y+ G  +GH AI  +D+Y   + I+A GG VTREPGPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 79.7221 bits (195), Expect = 1.733e-14
Identity = 36/66 (54.55%), Postives = 47/66 (71.21%), Query Frame = 2 
Query:  518 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEYTR 715
            +   MLRVGDL +SI+FY + +GM LLR  +  EYKYTLA LGY +E Q  V+E TYN+GV +Y +
Sbjct:    6 ILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEK 71
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.248e-38
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 2 
Query:  125 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 84.3445 bits (207), Expect = 7.037e-16
Identity = 40/64 (62.50%), Postives = 47/64 (73.44%), Query Frame = 2 
Query:  518 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEY 709
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+E TYN+GV +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.248e-38
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 2 
Query:  125 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 84.3445 bits (207), Expect = 7.037e-16
Identity = 40/64 (62.50%), Postives = 47/64 (73.44%), Query Frame = 2 
Query:  518 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEY 709
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+E TYN+GV +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.248e-38
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 2 
Query:  125 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 84.3445 bits (207), Expect = 7.037e-16
Identity = 40/64 (62.50%), Postives = 47/64 (73.44%), Query Frame = 2 
Query:  518 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEY 709
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+E TYN+GV +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 5.008e-38
Identity = 75/124 (60.48%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  125 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            RLLH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 82.0333 bits (201), Expect = 3.492e-15
Identity = 39/64 (60.94%), Postives = 45/64 (70.31%), Query Frame = 2 
Query:  518 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEY 709
            L   MLRVGDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+E TYN+GV  Y
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESY 66
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_SALTI (Lactoylglutathione lyase OS=Salmonella typhi GN=gloA PE=3 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 5.008e-38
Identity = 75/124 (60.48%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  125 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            RLLH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 82.0333 bits (201), Expect = 3.492e-15
Identity = 39/64 (60.94%), Postives = 45/64 (70.31%), Query Frame = 2 
Query:  518 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLEXTYNYGVTEY 709
            L   MLRVGDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+E TYN+GV  Y
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESY 66
[Close]
BLAST of FC920550 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 5.537e-37
Identity = 69/123 (56.10%), Postives = 91/123 (73.98%), Query Frame = 2 
Query:  128 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 496
            LLH + RVGDLD++++FY +  GM LLRK+D P  +++ AF+G+G E  + V+ELT+N+G   YD+G GFGH A+  ED+Y   + IR KGG V REPGP+K GTT IAFV+DPDGY  ELIQ
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIELIQ 125
The following BLAST results are available for this feature:
BLAST of FC920550 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
LGUL_ORYSJ1.385e-9681.95Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_BRAOG1.385e-9681.86Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_VIBPA2.837e-4162.90Probable lactoylglutathione lyase OS=Vibrio paraha... [more]
LGUL_VIBCH4.097e-4061.29Probable lactoylglutathione lyase OS=Vibrio choler... [more]
LGUL_SHIFL2.248e-3860.48Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI2.248e-3860.48Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO572.248e-3860.48Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_SALTY5.008e-3860.48Lactoylglutathione lyase OS=Salmonella typhimurium... [more]
LGUL_SALTI5.008e-3860.48Lactoylglutathione lyase OS=Salmonella typhi GN=gl... [more]
LGUL_SYNY35.537e-3756.10Probable lactoylglutathione lyase OS=Synechocystis... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC32009G12EF RVDevelop1 Citrus clementina cDNA clone C32009G12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920550 ID=FC920550; Name=FC920550; organism=Citrus clementina; type=EST; length=726bp
GAGATCATATCTCACACTTGCTCCTTCACTCCGACTTTCTGAGTGATTCC
ATATCAAAATGGCTGAGGCTTCTCCTGCTGCTGCTAATGCCGAGTTGCTG
GAATGGCCAAAGAAAGATAAGCGACGCCTCCTTCATGCTGTGTATCGTGT
TGGTGATCTTGATCGCACCATCAAATTTTACACCGAATGCTTTGGGATGA
AGCTGTTGAGAAAGAGAGACGTCCCGGAAGAGAAATACTCAAATGCCTTT
CTTGGATTTGGCCCCGAACAGTCTCACTTTGTTGTGGAGTTAACGTACAA
CTATGGTGTAACTTCATATGATATTGGAACTGGTTTTGGCCATTTTGCAA
TTGCAACTGAAGATGTTTACAAACTGGTTGAAAACATTCGTGCCAAGGGT
GGCAATGTCACTAGAGAGCCTGGTCCAGTTAAAGGTGGAACAACTCACAT
TGCCTTTGTGAAGGATCCCGATGGTTATATTTTTGAACTCATCCAGAGAG
GTCCAACTCCTGAACCACTCTGTCAAGTAATGCTTCGCGTTGGCGATCTT
GGTCGCTCCATCAAGTTCTATGAAAAGGCTTTGGGGATGAAACTGTTAAG
GACAGTTGATAAACCTGAATACAAGTACACCCTGGCCATGCTTGGATATG
CTGAGGAAGATCAGACTACTGTTCTGGAGNTAACTTATAATTATGGTGTG
ACTGAATATACCAGGGAAATGCATAT
back to top