FC920634

Overview
NameFC920634
Unique NameFC920634
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length689
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig10302 contig Ccv1_Contig10302:1..689. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 1.324e-69
Identity = 123/176 (69.89%), Postives = 141/176 (80.11%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRALASAGTEPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGPKPWPI+GNL HLGP PHQS A +A+T+GPLMYL++GF             QFLK HD+NFS+RPPNSGA+H+AYNYQD+VF PYGPRWRMLRKI SVHLFS KALDD+RHVRQ+E+  LTRALASAG +PV L Q LN+C  NAL RVMLG RVF DG+G  DP+A EFKSM
Sbjct:   34 PPGPKPWPIIGNLVHLGPKPHQSTAAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALARVMLGKRVFADGSGDVDPQAAEFKSM 209          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 7.038e-63
Identity = 109/176 (61.93%), Postives = 138/176 (78.41%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRALASAGTEPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGP PWPI+GNLPH+G  PH++++ +  T+GP+++LRLGF             QFLKIHD+NF++RPPNSGAKH+AYNYQD+VF PYG RWR+LRKISSVHLFS KAL+D++HVRQEE+  LTR L   GT+PVNL Q +N+CVVNALGR M+G R+FG     +D +ADEF+SM
Sbjct:   34 PPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREMIGRRLFG---ADADHKADEFRSM 206          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A5_EUSGR (Flavonoid 3',5'-hydroxylase OS=Eustoma grandiflorum GN=CYP75A5 PE=2 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 3.399e-41
Identity = 83/178 (46.63%), Postives = 119/178 (66.85%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRALASAGT--EPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGP  WP++G LP LG MPH ++A +A+ +GP+MYL++G               FLK  D NFSNRPPN+GA H+AYN QD+VF  YGPRW++LRK+S++H+  GKAL  +  VR++E+  +  A+A +G   +PV +++ L   + N LG+VML  RVF  G+ GS+  ++EFK M
Sbjct:   38 PPGPIGWPVLGALPLLGTMPHVALANMAKKYGPVMYLKVGSCGLAVASTPEAAKAFLKTLDMNFSNRPPNAGATHLAYNAQDMVFADYGPRWKLLRKLSNIHILGGKALQGWEEVRKKELGYMLYAMAESGRHGQPVVVSEMLTYAMANMLGQVMLSKRVF--GSQGSE--SNEFKDM 211          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A7_EUSGR (Flavonoid 3',5'-hydroxylase OS=Eustoma grandiflorum GN=CYP75A7 PE=2 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 3.758e-40
Identity = 82/178 (46.07%), Postives = 118/178 (66.29%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRALASAGT--EPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGP  WP++G L  LG MPH ++A +A+ +GP+MYL++G               FLK  D NFSNRPPN+GA H+AYN QD+VF  YGPRW++LRK+S++H+  GKAL  +  VR++E+  +  A+A +G   +PV +++ L   + N LG+VML  RVF  G+ GS+  ++EFK M
Sbjct:   38 PPGPIGWPVLGALRLLGTMPHVALANMAKKYGPVMYLKVGSCGLAVASTPEAAKAFLKTLDMNFSNRPPNAGATHLAYNAQDMVFADYGPRWKLLRKLSNIHILGGKALQGWEEVRKKELGYMLYAMAESGRHGQPVVVSEMLTYAMANMLGQVMLSKRVF--GSQGSE--SNEFKDM 211          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 3.181e-39
Identity = 79/178 (44.38%), Postives = 116/178 (65.17%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRAL--ASAGTEPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGP+ WP++G LP LG MPH ++A +A+ +GP+MYL++G               FLK  D NFSNRPPN+GA H+AYN QD+VF PYGPRW++LRK+S++H+  GKAL+++ +VR  E+  + +++  AS   E + +A  L   + N +G+VML  RVF +         +EFK+M
Sbjct:   37 PPGPEGWPVIGALPLLGGMPHVALAKMAKKYGPIMYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRPPNAGATHMAYNAQDMVFAPYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASHVGERIVVADMLTFAMANMIGQVMLSKRVFVE----KGKEVNEFKNM 210          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 2.693e-38
Identity = 78/178 (43.82%), Postives = 113/178 (63.48%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRALASAGTE--PVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGP+ WP++G LP LG MPH S+A +A+ +G +MYL++G               FLK  D NFSNRPPN+GA H+AYN QD+VF  YGPRW++LRK+S++H+  GKAL+++ +VR  E+  + ++++    E   V +A+ L   + N +G+VML  RVF D         +EFK M
Sbjct:   34 PPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGAIMYLKVGTCGMAVASTPDAAKAFLKTLDINFSNRPPNAGATHLAYNAQDMVFAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMSDMSREGQRVVVAEMLTFAMANMIGQVMLSKRVFVD----KGVEVNEFKDM 207          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A4_GENTR (Flavonoid 3',5'-hydroxylase OS=Gentiana triflora GN=CYP75A4 PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 7.835e-38
Identity = 77/178 (43.26%), Postives = 113/178 (63.48%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRAL--ASAGTEPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGP  WPI+G LP LG MPH + A +A+ +G +MYL++G               FLK  D NFSNRPPN+GA H+AYN QD+VF  YGP+W++LRK+S++H+  GKAL+++  VR+ E+  + +A+  +S   EPV +++ L   + N L +V+L  RVF         +++EFK M
Sbjct:   42 PPGPTGWPILGALPLLGNMPHVTFANMAKKYGSVMYLKVGSHGLAIASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYNAQDMVFAHYGPKWKLLRKLSNLHMLGGKALENWADVRKTELGYMLKAMFESSQNNEPVMISEMLTYAMANMLSQVILSRRVFNK----KGAKSNEFKDM 215          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.930e-36
Identity = 76/178 (42.70%), Postives = 112/178 (62.92%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRAL--ASAGTEPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSDPRADEFKSM 689
            PPGP+ WP++G LP LG MPH S+A +A+ +G +MYL++G               FLK  D NFSNRPPN+GA H+AY  QD+VF  YGPRW++LRK+S++H+  GKAL+++ +VR  E+  + +++   S   E V +A+ L   + N +G+V+L  RVF +         +EFK M
Sbjct:   34 PPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGAIMYLKVGTCGMVVASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYGAQDMVFAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDMSREGERVVVAEMLTFAMANMIGQVILSKRVFVN----KGVEVNEFKDM 207          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A6_CAMME (Flavonoid 3',5'-hydroxylase OS=Campanula medium GN=CYP75A6 PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 1.059e-34
Identity = 73/185 (39.46%), Postives = 108/185 (58.38%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRALASAGTE----PVNLAQRLNLCVVNALGRVMLGFRVFG-----DGTGGSDPRADEFKSM 689
            PPGP  WPI+G LP LG MPH S+A +A  +GP+MYL+LG               FLK HD+NFSNRP + G  ++AYN QD+VF  YGP+W++LRK+ S+H+   KAL+D+ HV+  E+  + + +    ++    PV + + L   + N +GR++L  R F      D +  +     EF+ M
Sbjct:   39 PPGPTGWPIIGALPLLGTMPHVSLADMAVKYGPIMYLKLGSKGTVVASNPKAARAFLKTHDANFSNRPIDGGPTYLAYNAQDMVFAEYGPKWKLLRKLCSLHMLGPKALEDWAHVKVSEVGHMLKEMYEQSSKSVPVPVVVPEMLTYAMANMIGRIILSRRPFVITSKLDSSASASASVSEFQYM 223          
BLAST of FC920634 vs. ExPASy Swiss-Prot
Match: C75A1_PINTA (Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 8.683e-29
Identity = 61/170 (35.88%), Postives = 98/170 (57.65%), Query Frame = 3
Query:  162 PPGPKPWPIVGNLPHLGPMPHQSIAGLARTHGPLMYLRLGFXXXXXXXXXXXXXQFLKIHDSNFSNRPPNSGAKHIAYNYQDIVFRPYGPRWRMLRKISSVHLFSGKALDDYRHVRQEEMAVLTRAL---ASAGTEPVNLAQRLNLCVVNALGRVMLGFRVFGDGTGGSD 662
            PPGP PWPI+GN   +    H+++  LA  +GP+++LR G               FLK HD  F++RPP S  K+  YN++DI F PYG  WR +RKI  + L + K ++ ++HVRQEE++ +  ++   + +G   VN+++ ++  + N L R+ L  + F D   G+D
Sbjct:   43 PPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILFLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRI-LARKKFSDNDLGAD 211          
The following BLAST results are available for this feature:
BLAST of FC920634 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 120
Match NameE-valueIdentityDescription
F3PH_PETHY1.324e-6969.89Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=C... [more]
F3PH_ARATH7.038e-6361.93Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana... [more]
C75A5_EUSGR3.399e-4146.63Flavonoid 3',5'-hydroxylase OS=Eustoma grandifloru... [more]
C75A7_EUSGR3.758e-4046.07Flavonoid 3',5'-hydroxylase OS=Eustoma grandifloru... [more]
C75A2_SOLME3.181e-3944.38Flavonoid 3',5'-hydroxylase OS=Solanum melongena G... [more]
C75A1_PETHY2.693e-3843.82Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida G... [more]
C75A4_GENTR7.835e-3843.26Flavonoid 3',5'-hydroxylase OS=Gentiana triflora G... [more]
C75A3_PETHY1.930e-3642.70Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida G... [more]
C75A6_CAMME1.059e-3439.46Flavonoid 3',5'-hydroxylase OS=Campanula medium GN... [more]
C75A1_PINTA8.683e-2935.88Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 P... [more]

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Properties
Property NameValue
Genbank descriptionC32011A09EF RVDevelop1 Citrus clementina cDNA clone C32011A09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920634 ID=FC920634; Name=FC920634; organism=Citrus clementina; type=EST; length=689bp
GGCGACTACTCGTACCATTTCTACACTTTCTCTGGGAAACGAAACAAAGA
GCAATGTCTACTTTACCACTACTGATACTGTACACATCACTTTTGGCAAT
TGTCATTTCTTTCCTTTTTAGCCTCCTCCGGAACAGACGCAGACACTCCT
CCCACCGTCTGCCTCCCGGTCCGAAACCCTGGCCAATAGTCGGAAACCTC
CCTCACTTGGGCCCTATGCCCCACCAATCCATAGCGGGTCTGGCCCGAAC
CCACGGCCCCCTCATGTACCTCCGGTTAGGCTTCGTTGACGTCGTCGTGG
CCGCTTCCGCTTCCGTGGCGGCCCAATTCCTGAAGATCCATGATTCCAAC
TTCTCCAACCGCCCTCCCAACTCCGGCGCCAAGCACATCGCTTACAACTA
TCAGGACATCGTTTTCCGGCCGTACGGCCCCCGCTGGCGGATGCTGAGGA
AGATCAGCTCCGTTCATCTTTTCTCCGGCAAGGCCTTGGATGACTACAGG
CACGTTCGCCAGGAAGAGATGGCGGTGCTCACGCGCGCGTTGGCGAGTGC
AGGAACGGAGCCGGTGAACTTAGCTCAACGGTTGAACTTGTGCGTTGTGA
ATGCCCTAGGGCGGGTGATGCTAGGCTTCAGAGTGTTCGGCGACGGTACC
GGAGGGAGCGACCCGAGGGCTGACGAGTTCAAATCAATG
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