GH733268

Overview
NameGH733268
Unique NameGH733268
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length252
Libraries
Library NameType
Citrus clementina pistils cDNA-AFLP librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP5_TOBAC (Luminal-binding protein 5 OS=Nicotiana tabacum GN=BIP5 PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 2.534e-25
Identity = 51/68 (75.00%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNMR+ INDK  LADK++SD+KE+IE+  KEALEWLDDNQ+AEK+D+DEK+KEVEAVCNP+IT
Sbjct:  574 LETYVYNMRNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKEDYDEKLKEVEAVCNPIIT 641

HSP 2 Score: 23.483 bits (49), Expect = 2.534e-25
Identity = 8/13 (61.54%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRNS 42
            +K+V+ER+D+RNS
Sbjct:  561 DKKVKERIDARNS 573
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP4_TOBAC (Luminal-binding protein 4 OS=Nicotiana tabacum GN=BIP4 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 7.269e-25
Identity = 50/68 (73.53%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNMR+ INDK  LADK++SD+KE+IE+  KEALEWLDDNQ+AEK+D++EK+KEVEAVCNP+IT
Sbjct:  575 LETYVYNMRNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKEDYEEKLKEVEAVCNPIIT 642

HSP 2 Score: 23.483 bits (49), Expect = 7.269e-25
Identity = 8/13 (61.54%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRNS 42
            +K+V+ER+D+RNS
Sbjct:  562 DKKVKERIDARNS 574
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP8_TOBAC (Luminal-binding protein 8 (Fragment) OS=Nicotiana tabacum GN=BIP8 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 7.358e-25
Identity = 50/68 (73.53%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNMR+ INDK  LADK++SD+KE+IE+  KEALEWLDDNQ+AEK+D++EK+KEVEAVCNP+IT
Sbjct:  201 LETYVYNMRNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKEDYEEKLKEVEAVCNPIIT 268

HSP 2 Score: 23.483 bits (49), Expect = 7.358e-25
Identity = 8/13 (61.54%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRNS 42
            +K+V+ER+D+RNS
Sbjct:  188 DKKVKERIDARNS 200
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP2_TOBAC (Luminal-binding protein 2 (Fragment) OS=Nicotiana tabacum GN=BIP2 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.622e-24
Identity = 49/68 (72.06%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNM++ INDK  LADK++SD+KE+IE+  KEALEWLDDNQ+AEK+D++EK+KEVEAVCNP+IT
Sbjct:  198 LETYVYNMKNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKEDYEEKLKEVEAVCNPIIT 265

HSP 2 Score: 23.483 bits (49), Expect = 1.622e-24
Identity = 8/13 (61.54%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRNS 42
            +K+V+ER+D+RNS
Sbjct:  185 DKKVKERIDARNS 197
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP1_TOBAC (Luminal-binding protein 1 (Fragment) OS=Nicotiana tabacum GN=BIP1 PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.111e-24
Identity = 48/68 (70.59%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNM++ INDK  LADK++SD+KE+IE+  KEALEWLDDNQ+AEK+D++EK+KEVEA+CNP+IT
Sbjct:  198 LETYVYNMKNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKEDYEEKLKEVEAICNPIIT 265

HSP 2 Score: 23.483 bits (49), Expect = 2.111e-24
Identity = 8/13 (61.54%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRNS 42
            +K+V+ER+D+RNS
Sbjct:  185 DKKVKERIDARNS 197
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP3_TOBAC (Luminal-binding protein 3 (Fragment) OS=Nicotiana tabacum GN=BIP3 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.771e-24
Identity = 49/68 (72.06%), Postives = 64/68 (94.12%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNM++ INDK  LADK++SD+KE+IE+ +KEALEWLDDNQ+AEK+D++EK+KEVEAVCNP+IT
Sbjct:   77 LETYVYNMKNQINDKDKLADKLESDEKEKIETAVKEALEWLDDNQSAEKEDYEEKLKEVEAVCNPIIT 144

HSP 2 Score: 21.9422 bits (45), Expect = 2.771e-24
Identity = 7/12 (58.33%), Postives = 12/12 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRN 39
            +K+V+ER+D+RN
Sbjct:   64 DKKVKERIDARN 75
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP_SOLLC (Luminal-binding protein OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.424e-24
Identity = 50/68 (73.53%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNM++ INDK  LADK++SD+KE+IE+  KEALEWLDDNQ+AEK+D+DEK+KEVEAVCNP+IT
Sbjct:  574 LETYVYNMKNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKEDYDEKLKEVEAVCNPIIT 641
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP_SPIOL (Luminal-binding protein OS=Spinacia oleracea GN=HSC70 PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 7.628e-24
Identity = 50/68 (73.53%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTIND--KLADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETYIYNM++ I+D  KLADK++SD+KE+IE  +KEALEWLDDNQ+AEK+D+DEK+KEVEAVCNP+IT
Sbjct:  574 LETYIYNMKNQISDADKLADKLESDEKEKIEGAVKEALEWLDDNQSAEKEDYDEKLKEVEAVCNPIIT 641
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP3_MAIZE (Luminal-binding protein 3 OS=Zea mays GN=BIPE3 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 6.394e-23
Identity = 44/68 (64.71%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNM++T+ DK  LADK+++++KE++E  LKEALEWLDDNQ+AEK+D++EK+KEVEAVCNP+++
Sbjct:  571 LETYVYNMKNTVGDKDKLADKLEAEEKEKVEEALKEALEWLDDNQSAEKEDYEEKLKEVEAVCNPIVS 638

HSP 2 Score: 21.9422 bits (45), Expect = 6.394e-23
Identity = 7/12 (58.33%), Postives = 12/12 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRN 39
            +K+V+ER+D+RN
Sbjct:  558 DKKVKERIDARN 569
[Close]
BLAST of GH733268 vs. ExPASy Swiss-Prot
Match: BIP2_MAIZE (Luminal-binding protein 2 OS=Zea mays GN=BIPE2 PE=1 SV=3)

HSP 1 Score: 104.76 bits (260), Expect = 6.394e-23
Identity = 44/68 (64.71%), Postives = 63/68 (92.65%), Query Frame = 3 
Query:   42 LETYIYNMRSTINDK--LADKIDSDDKERIESTLKEALEWLDDNQNAEKDDFDEKMKEVEAVCNPVIT 239
            LETY+YNM++T+ DK  LADK+++++KE++E  LKEALEWLDDNQ+AEK+D++EK+KEVEAVCNP+++
Sbjct:  571 LETYVYNMKNTVGDKDKLADKLEAEEKEKVEEALKEALEWLDDNQSAEKEDYEEKLKEVEAVCNPIVS 638

HSP 2 Score: 21.9422 bits (45), Expect = 6.394e-23
Identity = 7/12 (58.33%), Postives = 12/12 (100.00%), Query Frame = 1 
Query:    4 EKEVRERVDSRN 39
            +K+V+ER+D+RN
Sbjct:  558 DKKVKERIDARN 569
The following BLAST results are available for this feature:
BLAST of GH733268 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 105
Match NameE-valueIdentityDescription
BIP5_TOBAC2.534e-2575.00Luminal-binding protein 5 OS=Nicotiana tabacum GN=... [more]
BIP4_TOBAC7.269e-2573.53Luminal-binding protein 4 OS=Nicotiana tabacum GN=... [more]
BIP8_TOBAC7.358e-2573.53Luminal-binding protein 8 (Fragment) OS=Nicotiana ... [more]
BIP2_TOBAC1.622e-2472.06Luminal-binding protein 2 (Fragment) OS=Nicotiana ... [more]
BIP1_TOBAC2.111e-2470.59Luminal-binding protein 1 (Fragment) OS=Nicotiana ... [more]
BIP3_TOBAC2.771e-2472.06Luminal-binding protein 3 (Fragment) OS=Nicotiana ... [more]
BIP_SOLLC3.424e-2473.53Luminal-binding protein OS=Solanum lycopersicum PE... [more]
BIP_SPIOL7.628e-2473.53Luminal-binding protein OS=Spinacia oleracea GN=HS... [more]
BIP3_MAIZE6.394e-2364.71Luminal-binding protein 3 OS=Zea mays GN=BIPE3 PE=... [more]
BIP2_MAIZE6.394e-2364.71Luminal-binding protein 2 OS=Zea mays GN=BIPE2 PE=... [more]

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Properties
Property NameValue
Genbank descriptionU_56 Citrus clementina pistils cDNA-AFLP library Citrus clementina cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>GH733268 ID=GH733268; Name=GH733268; organism=Citrus clementina; type=EST; length=252bp
GNGGAGAAAGAGGTGAGGGAGAGGGTTGATTCCAGGAACAGTTAGAAACT
TACATCTACAACATGAGGAGCACCATCAATGACAAGCTTGCGGACAAGAT
TGATTCTGATGATAAGGAGAGGATTGAGAGCACACTGAAGGAAGCTCTCG
AATGGTTGGATGATAATCAGAACGCAGAGAAGGATGATTTTGACGAGAAG
ATGAAGGAGGTGGAAGCTGTATGCAACCCTGTGATTACTCAGGACTCATC
AN
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