BQ623072

Overview
NameBQ623072
Unique NameBQ623072
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length731
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1L_ARATH (Rae1-like protein At1g80670 OS=Arabidopsis thaliana GN=At1g80670 PE=2 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 2.155e-72
Identity = 95/113 (84.07%), Postives = 106/113 (93.81%), Query Frame = 1 
Query:  115 FKCHRDGSEIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYLHLPQETEVKGKPRAGTSGRK 453
            FKCHRDG++IYSVNSLNFHP+H TFATAGSDGAFNFWDKDSKQRLKAMSRC QPIPCS+FN+DGSI+AY+ CYDWSKGAENHNPATAK+ I+LHLPQE+EVK KPR G +GRK
Sbjct:  237 FKCHRDGNDIYSVNSLNFHPVHGTFATAGSDGAFNFWDKDSKQRLKAMSRCNQPIPCSSFNHDGSIYAYAACYDWSKGAENHNPATAKSSIFLHLPQESEVKAKPRVGATGRK 349

HSP 2 Score: 78.1814 bits (191), Expect = 2.155e-72
Identity = 36/38 (94.74%), Postives = 36/38 (94.74%), Query Frame = 2 
Query:    2 TRCVAAFPDQQGFLVGSIEGRVGVHHLDDSQQSKNFHF 115
            TRCV AFPDQQGFLVGSIEGRVGVHHLDDSQQSKNF F
Sbjct:  200 TRCVTAFPDQQGFLVGSIEGRVGVHHLDDSQQSKNFTF 237
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1_SCHPO (Poly(A)+ RNA export protein OS=Schizosaccharomyces pombe GN=rae1 PE=1 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.187e-31
Identity = 58/112 (51.79%), Postives = 74/112 (66.07%), Query Frame = 1 
Query:  115 FKCHRD----GSEIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYLHLPQETEVKGKPRAG 438
            F+CHR+     +++YSVNS+ FHP + TF+TAGSDG F+FWDKDS QRLK+    G  I CSTFN  G IFAY++ YDWSKG    N A     I LH   + E+K +P+ G
Sbjct:  241 FRCHRNQAGNSADVYSVNSIAFHPQYGTFSTAGSDGTFSFWDKDSHQRLKSYPNVGGTISCSTFNRTGDIFAYAISYDWSKG-YTFNNAQLPNKIMLHPVPQDEIKPRPKKG 351

HSP 2 Score: 38.5058 bits (88), Expect = 1.187e-31
Identity = 15/38 (39.47%), Postives = 24/38 (63.16%), Query Frame = 2 
Query:    2 TRCVAAFPDQQGFLVGSIEGRVGVHHLDDSQQSKNFHF 115
            TR +A F    G+ +GS+EGR  + ++D+   S+NF F
Sbjct:  204 TRSLACFIKGDGYAIGSVEGRCAIQNIDEKNASQNFSF 241
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1L_XENTR (mRNA export factor OS=Xenopus tropicalis GN=rae1 PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 8.103e-30
Identity = 52/100 (52.00%), Postives = 67/100 (67.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN + FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI   +FN++G+IFAYS  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTTAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACSFNHNGNIFAYSSSYDWSKGHEFYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 8.103e-30
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1L_DANRE (mRNA export factor OS=Danio rerio GN=rae1 PE=2 SV=2)

HSP 1 Score: 111.309 bits (277), Expect = 8.103e-30
Identity = 51/100 (51.00%), Postives = 68/100 (68.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN+++FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI    FN++G+IFAY+  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTATPQDIYAVNAISFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPITACCFNHNGNIFAYASSYDWSKGHEYYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 8.103e-30
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQSKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAEL1_PONAB (mRNA export factor OS=Pongo abelii GN=RAE1 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.032e-29
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN + FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI    FN++G+IFAY+  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 3.032e-29
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAEL1_PIG (mRNA export factor OS=Sus scrofa GN=RAE1 PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.032e-29
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN + FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI    FN++G+IFAY+  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 3.032e-29
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1L_MOUSE (mRNA export factor OS=Mus musculus GN=Rae1 PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.032e-29
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN + FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI    FN++G+IFAY+  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPIAACCFNHNGNIFAYASSYDWSKGHEFYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 3.032e-29
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1L_MACFA (mRNA export factor OS=Macaca fascicularis GN=RAE1 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.032e-29
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN + FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI    FN++G+IFAY+  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 3.032e-29
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1L_HUMAN (mRNA export factor OS=Homo sapiens GN=RAE1 PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.032e-29
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN + FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI    FN++G+IFAY+  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 3.032e-29
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
[Close]
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Match: RAE1L_BOVIN (mRNA export factor OS=Bos taurus GN=RAE1 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.032e-29
Identity = 51/100 (51.00%), Postives = 66/100 (66.00%), Query Frame = 1 
Query:  115 FKCHRDGS-------EIYSVNSLNFHPIHHTFATAGSDGAFNFWDKDSKQRLKAMSRCGQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIYL 393
            FKCHR          +IY+VN + FHP+H T AT GSDG F+FWDKD++ +LK   +  QPI    FN++G+IFAY+  YDWSKG E +NP   K YI+L
Sbjct:  257 FKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNP-QKKNYIFL 355

HSP 2 Score: 40.4318 bits (93), Expect = 3.032e-29
Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:    5 RCVAAFPDQQ----GFLVGSIEGRVGVHHLDDSQQSK-NF----HFSNAT 127
            RCVA F D+Q    GF +GSIEGRV +H+++    +K NF    H SN T
Sbjct:  216 RCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGT 265
The following BLAST results are available for this feature:
BLAST of BQ623072 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19
Match NameE-valueIdentityDescription
RAE1L_ARATH2.155e-7284.07Rae1-like protein At1g80670 OS=Arabidopsis thalian... [more]
RAE1_SCHPO1.187e-3151.79Poly(A)+ RNA export protein OS=Schizosaccharomyces... [more]
RAE1L_XENTR8.103e-3052.00mRNA export factor OS=Xenopus tropicalis GN=rae1 P... [more]
RAE1L_DANRE8.103e-3051.00mRNA export factor OS=Danio rerio GN=rae1 PE=2 SV=... [more]
RAEL1_PONAB3.032e-2951.00mRNA export factor OS=Pongo abelii GN=RAE1 PE=2 SV... [more]
RAEL1_PIG3.032e-2951.00mRNA export factor OS=Sus scrofa GN=RAE1 PE=3 SV=1[more]
RAE1L_MOUSE3.032e-2951.00mRNA export factor OS=Mus musculus GN=Rae1 PE=1 SV... [more]
RAE1L_MACFA3.032e-2951.00mRNA export factor OS=Macaca fascicularis GN=RAE1 ... [more]
RAE1L_HUMAN3.032e-2951.00mRNA export factor OS=Homo sapiens GN=RAE1 PE=1 SV... [more]
RAE1L_BOVIN3.032e-2951.00mRNA export factor OS=Bos taurus GN=RAE1 PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00163 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00163 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623072 ID=BQ623072; Name=BQ623072; organism=Citrus sinensis; type=EST; length=731bp
GACGAGATGTGTTGCTGCCTTTCCTGACCAGCAAGGTTTCTTGGTTGGTT
CAATTGAGGGAAGGGTTGGTGTTCATCATCTTGATGATTCACAGCAAAGT
AAGAACTTTCACTTTTCAAATGCCACAGAGATGGTAGTGAGATATACTCA
GTCAACTCATTGAACTTCCACCCTATACATCACACTTTTGCAACCGCTGG
ATCTGATGGTGCATTTAATTTTTGGGACAAGGATAGTAAACAGCGGCTCA
AGGCCATGTCAAGGTGCGGGCAACCTATACCTTGCAGTACATTCAATAAT
GATGGCTCAATATTTGCGTACTCGGTGTGTTATGACTGGAGTAAGGGTGC
AGAAAATCACAACCCTGCAACAGCAAAGACTTACATCTATCTGCACTTGC
CACAGGAGACTGAGGTTAAAGGCAAACCACGGGCTGGGACAAGCGGGAGA
AAGTAAAGCTGCTTTTGGGCTGAGAGCATTACATATGGAAGCTTTATGAT
GAGAATTTCCTACCACCCCCTACCCCCTTCATGTCACGATAGCATTTTCT
GTTTCTTCCCAGCATTCGTAAATGAATGAGATGTGGGTTCTTGTTGTCAA
TAGAGAAGTCTAGATTAGGATTTAGGAATGAGGGTATTGAGAGGGTCAAT
GTTTTTAATATCCCTGGCCAGAGATGAAATTTTTCCAGATGAGCATTAAA
TTTAATCTTTTGACTTAGTTGTTTCAATGCT
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