BQ623325

Overview
NameBQ623325
Unique NameBQ623325
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length512
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig4610 contig Csv1_Contig4610:526..1036. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP24_ARATH (Probable aquaporin PIP2-4 OS=Arabidopsis thaliana GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 3.257e-70
Identity = 119/130 (91.54%), Postives = 123/130 (94.62%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGANELADGY+ GTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARS GAAVIY+ EKAWDDQWIFWVGP IGAA AAFYHQFILRA+A KALGSF S
Sbjct:  154 YGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNEKAWDDQWIFWVGPMIGAAAAAFYHQFILRAAAIKALGSFGS 283

HSP 2 Score: 39.2762 bits (90), Expect = 3.257e-70
Identity = 16/18 (88.89%), Postives = 16/18 (88.89%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICGCG VKAFQ SYY
Sbjct:  134 LGAICGCGFVKAFQSSYY 151
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP22_ARATH (Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 246.514 bits (628), Expect = 3.554e-69
Identity = 117/130 (90.00%), Postives = 123/130 (94.62%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGAN LADGY+TGTGL AEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARS GAAVIY+K K WDD WIFWVGPFIGAAIAAFYHQF+LRAS SK+LGSF+S
Sbjct:  152 YGGGANSLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRS 281

HSP 2 Score: 35.4242 bits (80), Expect = 3.554e-69
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG G VKAFQ SYY
Sbjct:  132 LGAICGVGFVKAFQSSYY 149
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP21_ARATH (Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 6.044e-69
Identity = 117/130 (90.00%), Postives = 123/130 (94.62%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGAN LADGYSTGTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARS GAAVIY+K K WDD WIFWVGPFIGAAIAAFYHQF+LRAS SK+LGSF+S
Sbjct:  154 YGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRS 283

HSP 2 Score: 35.4242 bits (80), Expect = 6.044e-69
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG G VKAFQ SYY
Sbjct:  134 LGAICGVGFVKAFQSSYY 151
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP21_MAIZE (Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 243.432 bits (620), Expect = 6.591e-68
Identity = 117/130 (90.00%), Postives = 122/130 (93.85%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGAN LA GYS GTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIY+K+K WDD WIFWVGP +GAAIAAFYHQ+ILRA A KALGSF+S
Sbjct:  159 YGGGANSLASGYSRGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYNKDKPWDDHWIFWVGPLVGAAIAAFYHQYILRAGAIKALGSFRS 288

HSP 2 Score: 34.2686 bits (77), Expect = 6.591e-68
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG GLVKAFQ +Y+
Sbjct:  139 LGAICGVGLVKAFQSAYF 156
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP23_ARATH (Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 242.276 bits (617), Expect = 1.121e-67
Identity = 115/130 (88.46%), Postives = 122/130 (93.85%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGAN LADGY+TGTGL AEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARS GAAVI++K K WDD WIFWVGPFIGA IAAFYHQF+LRAS SK+LGSF+S
Sbjct:  152 YGGGANFLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRS 281

HSP 2 Score: 34.6538 bits (78), Expect = 1.121e-67
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYYI 57
            LGAICG G VKAFQ S+Y+
Sbjct:  132 LGAICGVGFVKAFQSSHYV 150
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP22_ORYSJ (Probable aquaporin PIP2-2 OS=Oryza sativa subsp. japonica GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 2.486e-67
Identity = 115/130 (88.46%), Postives = 125/130 (96.15%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGANEL+DGYS GTGLGAEIIGTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAVFMVHLATIP+TGTGINPARSLG AVIY+K+KAWDDQWIFWVGP IGAAIAA YHQ++LRASA+K LGS++S
Sbjct:  158 YGGGANELSDGYSKGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGTAVIYNKDKAWDDQWIFWVGPLIGAAIAAAYHQYVLRASAAK-LGSYRS 286

HSP 2 Score: 35.4242 bits (80), Expect = 2.486e-67
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG GLVK FQ SYY
Sbjct:  138 LGAICGVGLVKGFQSSYY 155
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP22_MAIZE (Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 7.188e-67
Identity = 114/130 (87.69%), Postives = 121/130 (93.08%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGAN LA GYS G GLGAEI+GTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAV+Y+K+K WDD WIFWVGP +GAAIAAFYHQ+ILRA A KALGSF+S
Sbjct:  161 YGGGANSLASGYSRGAGLGAEIVGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVVYNKDKPWDDHWIFWVGPLLGAAIAAFYHQYILRAGAIKALGSFRS 290

HSP 2 Score: 33.8834 bits (76), Expect = 7.188e-67
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYY 54
            LGA+CG GLVKAFQ +Y+
Sbjct:  141 LGAVCGVGLVKAFQSAYF 158
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP23_MAIZE (Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 1.222e-66
Identity = 115/130 (88.46%), Postives = 125/130 (96.15%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGANEL+DGYS GTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARSLGAAVIY+K+KAWDDQWIFWVGP IGAAIAA YHQ++LRASA+K LGS++S
Sbjct:  159 YGGGANELSDGYSKGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYNKDKAWDDQWIFWVGPLIGAAIAAAYHQYVLRASATK-LGSYRS 287

HSP 2 Score: 35.4242 bits (80), Expect = 1.222e-66
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYYI 57
            LGAICG GLVK FQ +YY+
Sbjct:  139 LGAICGVGLVKGFQSAYYV 157
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP24_MAIZE (Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 1.222e-66
Identity = 115/130 (88.46%), Postives = 125/130 (96.15%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGANEL+DGYS GTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARSLGAAVIY+K+KAWDDQWIFWVGP IGAAIAA YHQ++LRASA+K LGS++S
Sbjct:  158 YGGGANELSDGYSKGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYNKDKAWDDQWIFWVGPLIGAAIAAAYHQYVLRASATK-LGSYRS 286

HSP 2 Score: 35.4242 bits (80), Expect = 1.222e-66
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYYI 57
            LGAICG GLVK FQ +YY+
Sbjct:  138 LGAICGVGLVKGFQSAYYV 156
[Close]
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: PIP21_ORYSJ (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 1.022e-65
Identity = 113/130 (86.92%), Postives = 121/130 (93.08%), Query Frame = 2 
Query:   62 YGGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASASKALGSFKS 451
            YGGGAN LA GYS GTGL AEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARS+GAAVI++ EKAW + WIFWVGPF+GAAIAAFYHQ+ILRA A KALGSF+S
Sbjct:  159 YGGGANTLAAGYSKGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSIGAAVIFNNEKAWHNHWIFWVGPFVGAAIAAFYHQYILRAGAIKALGSFRS 288

HSP 2 Score: 34.2686 bits (77), Expect = 1.022e-65
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = 1 
Query:    1 LGAICGCGLVKAFQKSYY 54
            LGAICG GLVKAFQ +Y+
Sbjct:  139 LGAICGVGLVKAFQSAYF 156
The following BLAST results are available for this feature:
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 132
Match NameE-valueIdentityDescription
PIP24_ARATH3.257e-7091.54Probable aquaporin PIP2-4 OS=Arabidopsis thaliana ... [more]
PIP22_ARATH3.554e-6990.00Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2... [more]
PIP21_ARATH6.044e-6990.00Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1... [more]
PIP21_MAIZE6.591e-6890.00Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2[more]
PIP23_ARATH1.121e-6788.46Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3... [more]
PIP22_ORYSJ2.486e-6788.46Probable aquaporin PIP2-2 OS=Oryza sativa subsp. j... [more]
PIP22_MAIZE7.188e-6787.69Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1[more]
PIP23_MAIZE1.222e-6688.46Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1[more]
PIP24_MAIZE1.222e-6688.46Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1[more]
PIP21_ORYSJ1.022e-6586.92Probable aquaporin PIP2-1 OS=Oryza sativa subsp. j... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00416 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00416 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623325 ID=BQ623325; Name=BQ623325; organism=Citrus sinensis; type=EST; length=512bp
TTGGGAGCCATATGTGGCTGCGGGCTCGTTAAGGCTTTCCAGAAGTCTTA
CTACATCGAGATACGGCGGTGGAGCCAATGAGCTCGCTGATGGGTACAGC
ACTGGCACTGGATTGGGTGCTGAGATCATCGGTACCTTTGTTCTTGTCTA
CACTGTTTTCTCCGCCACTGATCCCAAGAGAAATGCTAGGGACTCCCACG
TTCCCGTCTTGGCGCCATTGCCAATTGGATTTGCTGTGTTCATGGTTCAC
TTGGCTACGATCCCTGTCACTGGAACTGGCATCAACCCCGCTAGAAGTTT
GGGAGCTGCAGTGATTTACAGCAAGGAGAAGGCTTGGGATGATCAATGGA
TCTTCTGGGTTGGCCCCTTCATTGGTGCTGCAATTGCTGCATTTTACCAC
CAATTCATCCTCAGAGCATCTGCTTCTAAAGCTCTTGGATCCTTCAAAAG
CCTCCTCCAACATTTAAATTAACTTTTAAAATAATTACTGGAGGATTAAT
CAATGGGTGTCA
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