BQ623508

Overview
NameBQ623508
Unique NameBQ623508
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length649
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2169 contig Csv1_Contig2169:929..1577. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_ORYSJ (Elongation factor 1-alpha OS=Oryza sativa subsp. japonica GN=REFA1 PE=2 SV=2)

HSP 1 Score: 272.707 bits (696), Expect = 4.635e-80
Identity = 133/141 (94.33%), Postives = 140/141 (99.29%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VASNSKDDPA+EAA+FTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+ELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIK+VEKKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASNSKDDPAKEAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKNVEKKDPTG 436

HSP 2 Score: 46.595 bits (109), Expect = 4.635e-80
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEALPGDNVG
Sbjct:  277 VKSVEMHHEALQEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_VICFA (Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 6.045e-80
Identity = 133/141 (94.33%), Postives = 140/141 (99.29%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRGFVASNSKDDPA+EAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+EL+TKIDRRSGKE+EKEPKFLKNGDAGMVKMIPTKPMVVETF++YPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGFVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELITKIDRRSGKEIEKEPKFLKNGDAGMVKMIPTKPMVVETFAEYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 436

HSP 2 Score: 44.2838 bits (103), Expect = 6.045e-80
Identity = 20/21 (95.24%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEAL EALPGDNVG
Sbjct:  277 VKSVEMHHEALTEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_MAIZE (Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 2.282e-79
Identity = 132/141 (93.62%), Postives = 138/141 (97.87%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VAS SKDDPA+EAA+FTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+ELVTKIDRRSGKELEKEPKFLKNGDAGMVKM+PTKPMVVETFS YPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASGSKDDPAKEAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKELEKEPKFLKNGDAGMVKMVPTKPMVVETFSQYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 436

HSP 2 Score: 46.595 bits (109), Expect = 2.282e-79
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEALPGDNVG
Sbjct:  277 VKSVEMHHEALQEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_MANES (Elongation factor 1-alpha OS=Manihot esculenta GN=EF1 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.466e-78
Identity = 131/141 (92.91%), Postives = 137/141 (97.16%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG VASNSKDDPA+EAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E++TKIDRRSGKELEKEPKFLKNGDAG VKMIPTKPMVVETFS YPPLGRFAVRDMRQTVAVGVIKSVEKKDP+G
Sbjct:  296 VGFNVKNVAVKDLKRGIVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSGKELEKEPKFLKNGDAGFVKMIPTKPMVVETFSAYPPLGRFAVRDMRQTVAVGVIKSVEKKDPSG 436

HSP 2 Score: 46.595 bits (109), Expect = 1.466e-78
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEALPGDNVG
Sbjct:  277 VKSVEMHHEALQEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_TOBAC (Elongation factor 1-alpha OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 267.314 bits (682), Expect = 1.911e-78
Identity = 129/141 (91.49%), Postives = 139/141 (98.58%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRGFVASNSKDDPA+ AA+FTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E++TKIDRRSGKE+EKEPKFLKNGDAGMVKMIPTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIK+V+KKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGFVASNSKDDPAKGAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKNVDKKDPTG 436

HSP 2 Score: 46.595 bits (109), Expect = 1.911e-78
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEALPGDNVG
Sbjct:  277 VKSVEMHHEALQEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A2_DAUCA (Elongation factor 1-alpha OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 2.493e-78
Identity = 130/141 (92.20%), Postives = 136/141 (96.45%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VASNSKDDPA+EAANFT+QVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E+ TKIDRRSGKELEKEPKFLKNGDAG VKMIPTKPMVVETF  YPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTAQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEIQTKIDRRSGKELEKEPKFLKNGDAGFVKMIPTKPMVVETFMSYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 436

HSP 2 Score: 46.595 bits (109), Expect = 2.493e-78
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEALPGDNVG
Sbjct:  277 VKSVEMHHEALQEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana GN=A1 PE=1 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 1.227e-77
Identity = 130/141 (92.20%), Postives = 138/141 (97.87%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VASNSKDDPA+ AANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSE++TKIDRRSGKE+EKEPKFLKNGDAGMVKM PTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIKSV+KKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKKDPTG 436

HSP 2 Score: 42.743 bits (99), Expect = 1.227e-77
Identity = 19/21 (90.48%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE+L EALPGDNVG
Sbjct:  277 VKSVEMHHESLLEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_PEA (Elongation factor 1-alpha OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 1.601e-77
Identity = 130/139 (93.53%), Postives = 137/139 (98.56%), Query Frame = 2 
Query:   65 FNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            FNVKNVAVKDLK G VASNSKDDPA++AANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+EL+TKIDRRSGKE+EKEPKFLKNGDAGMVKMIPTKPMVVETF++YPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG
Sbjct:  298 FNVKNVAVKDLKHGLVASNSKDDPAKDAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELITKIDRRSGKEIEKEPKFLKNGDAGMVKMIPTKPMVVETFAEYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 436

HSP 2 Score: 41.9726 bits (97), Expect = 1.601e-77
Identity = 19/20 (95.00%), Postives = 19/20 (95.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNV 60
            VKSVEMHHEAL EALPGDNV
Sbjct:  277 VKSVEMHHEALTEALPGDNV 296
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_SOLLC (Elongation factor 1-alpha OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 1.601e-77
Identity = 126/141 (89.36%), Postives = 139/141 (98.58%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VASNSKDDPA+ AA+FT+QVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E++TKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETF++YPPLGRFAVRDMRQTVAVGV+K+V+KKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASNSKDDPAKGAASFTAQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFAEYPPLGRFAVRDMRQTVAVGVVKNVDKKDPTG 436

HSP 2 Score: 46.595 bits (109), Expect = 1.601e-77
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHEALQEALPGDNVG
Sbjct:  277 VKSVEMHHEALQEALPGDNVG 297
[Close]
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Match: EF1A_SOYBN (Elongation factor 1-alpha OS=Glycine max GN=TEFS1 PE=3 SV=2)

HSP 1 Score: 268.855 bits (686), Expect = 4.633e-77
Identity = 130/141 (92.20%), Postives = 139/141 (98.58%), Query Frame = 2 
Query:   59 LAFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 481
            + FNVKNVAVKDLKRG+VASNSKDDPA+EAANFT+QVII+NHPGQIGNGYAPVLDCHTSHIAVKF+EL+TKIDRRSGKELEKEPKFLKNGDAG VKMIPTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIK+VEKKDPTG
Sbjct:  296 VGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTAQVIIINHPGQIGNGYAPVLDCHTSHIAVKFAELMTKIDRRSGKELEKEPKFLKNGDAGFVKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKNVEKKDPTG 436

HSP 2 Score: 40.4318 bits (93), Expect = 4.633e-77
Identity = 18/21 (85.71%), Postives = 19/21 (90.48%), Query Frame = 1 
Query:    1 VKSVEMHHEALQEALPGDNVG 63
            VKSVEMHHE+L EA PGDNVG
Sbjct:  277 VKSVEMHHESLTEAHPGDNVG 297
The following BLAST results are available for this feature:
BLAST of BQ623508 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A_ORYSJ4.635e-8094.33Elongation factor 1-alpha OS=Oryza sativa subsp. j... [more]
EF1A_VICFA6.045e-8094.33Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1[more]
EF1A_MAIZE2.282e-7993.62Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3... [more]
EF1A_MANES1.466e-7892.91Elongation factor 1-alpha OS=Manihot esculenta GN=... [more]
EF1A_TOBAC1.911e-7891.49Elongation factor 1-alpha OS=Nicotiana tabacum PE=... [more]
EF1A2_DAUCA2.493e-7892.20Elongation factor 1-alpha OS=Daucus carota PE=2 SV... [more]
EF1A_ARATH1.227e-7792.20Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
EF1A_PEA1.601e-7793.53Elongation factor 1-alpha OS=Pisum sativum PE=2 SV... [more]
EF1A_SOLLC1.601e-7789.36Elongation factor 1-alpha OS=Solanum lycopersicum ... [more]
EF1A_SOYBN4.633e-7792.20Elongation factor 1-alpha OS=Glycine max GN=TEFS1 ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00599 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00599 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623508 ID=BQ623508; Name=BQ623508; organism=Citrus sinensis; type=EST; length=649bp
GTTAAGTCTGTTGAGATGCACCACGAAGCTCTGCAGGAGGCCCTTCCGGG
TGACAATGTTGGCCTTCAACGTGAAGAACGTTGCTGTCAAGGATCTCAAG
CGTGGTTTTGTTGCTTCCAACTCGAAGGATGATCCGGCCAGGGAAGCTGC
CAACTTCACTTCACAGGTCATCATCATGAACCATCCTGGCCAGATTGGAA
ATGGATATGCCCCAGTGCTTGACTGCCACACCTCCCACATTGCCGTCAAG
TTTTCTGAGCTCGTGACGAAGATTGATAGGCGATCAGGCAAGGAGCTTGA
GAAGGAGCCCAAGTTCTTGAAGAATGGAGATGCCGGAATGGTTAAGATGA
TTCCCACCAAGCCCATGGTTGTTGAGACATTCTCCGACTACCCACCCCTT
GGTCGTTTTGCTGTTAGGGACATGCGTCAGACTGTTGCTGTGGGTGTTAT
CAAGAGTGTTGAGAAGAAGGATCCCACAGGTGCCAAGGTCACCAAGTCCG
CAGCCAAGAAGAAATGAGAAATTTTTATGGGTCTGTCTACAATAGTTAGG
GTTTATTTTCTGGCTTTTTTATTTTCTTTTCTGGTTTTGGTTTGCATCAA
TGTTTCTGCAAACAGCCCTATTTGCTTCCACCAGTTTGCTGGTGAGCAT
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