CF831987

Overview
NameCF831987
Unique NameCF831987
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length878
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF831987_ssr19 microsatellite CF831987_ssr19:19..30. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig1001 contig Csv1_Contig1001:724..1601. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_BETVU (Elongation factor 2 OS=Beta vulgaris PE=2 SV=1)

HSP 1 Score: 318.161 bits (814), Expect = 3.979e-86
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGALAEENMRGICFEVCDVVLH DAIHRGGGQ+IPTARRV YASQLTAKPRLLEPVY+VEIQAPE ALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPV+ESFGFS TLRA+TSGQAFPQCVFDHW+MM SDPLE G+QA+ LV+ IRKRKGLKEQMTPLSEFEDKL
Sbjct:  674 EGALAEENMRGICFEVCDVVLHTDAIHRGGGQIIPTARRVFYASQLTAKPRLLEPVYLVEIQAPENALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSTLRASTSGQAFPQCVFDHWEMMPSDPLEAGSQASTLVSVIRKRKGLKEQMTPLSEFEDKL 843
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_CHLKE (Elongation factor 2 OS=Chlorella kessleri PE=2 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 8.316e-76
Identity = 135/170 (79.41%), Postives = 153/170 (90.00%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EG LAEENMRGI FEVCDVVLHADAIHRGGGQ+IPTARR +YA+QLTA+PRLLEPVY+VEIQ PEQA+GG+YSVLNQKRG VFEE+QRPGTP++N+KAYLPVIESFGF+ TLRAAT+GQAFPQCVFDHW+ M SDP + G+QA  LV DIRKRKGLK +   LSE+EDKL
Sbjct:  676 EGVLAEENMRGIVFEVCDVVLHADAIHRGGGQIIPTARRSMYAAQLTAQPRLLEPVYLVEIQCPEQAMGGVYSVLNQKRGMVFEELQRPGTPIFNLKAYLPVIESFGFTSTLRAATAGQAFPQCVFDHWEAMGSDPTQVGSQANTLVMDIRKRKGLKPEPAALSEYEDKL 845
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_CAEEL (Elongation factor 2 OS=Caenorhabditis elegans GN=eft-2 PE=2 SV=4)

HSP 1 Score: 243.047 bits (619), Expect = 1.627e-63
Identity = 116/170 (68.24%), Postives = 137/170 (80.59%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EG L++ENMRG+ F V DV LHADAIHRGGGQ+IPTARRV YAS LTA+PRLLEPVY+VEIQ PE A+GGIY VLN++RGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DPLE GT+  Q+V D RKRKGLKE +  L  + DK+
Sbjct:  683 EGVLSDENMRGVRFNVHDVTLHADAIHRGGGQIIPTARRVFYASVLTAEPRLLEPVYLVEIQCPEAAVGGIYGVLNRRRGHVFEESQVTGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPLEAGTKPNQIVLDTRKRKGLKEGVPALDNYLDKM 852
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_RAT (Elongation factor 2 OS=Rattus norvegicus GN=Eef2 PE=1 SV=4)

HSP 1 Score: 241.891 bits (616), Expect = 3.624e-63
Identity = 114/170 (67.06%), Postives = 138/170 (81.18%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  689 EGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_PONAB (Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 SV=3)

HSP 1 Score: 241.891 bits (616), Expect = 3.624e-63
Identity = 114/170 (67.06%), Postives = 138/170 (81.18%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  689 EGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_MOUSE (Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 SV=2)

HSP 1 Score: 241.891 bits (616), Expect = 3.624e-63
Identity = 114/170 (67.06%), Postives = 138/170 (81.18%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  689 EGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_MESAU (Elongation factor 2 OS=Mesocricetus auratus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 241.891 bits (616), Expect = 3.624e-63
Identity = 114/170 (67.06%), Postives = 138/170 (81.18%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  689 EGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_HUMAN (Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4)

HSP 1 Score: 241.891 bits (616), Expect = 3.624e-63
Identity = 114/170 (67.06%), Postives = 138/170 (81.18%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  689 EGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_CALJA (Elongation factor 2 OS=Callithrix jacchus GN=EEF2 PE=2 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 6.181e-63
Identity = 114/170 (67.06%), Postives = 138/170 (81.18%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  689 EGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF831987 vs. ExPASy Swiss-Prot
Match: EF2_BOVIN (Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 241.121 bits (614), Expect = 6.181e-63
Identity = 114/170 (67.06%), Postives = 138/170 (81.18%), Query Frame = -2 
Query:  368 EGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 877
            EGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  689 EGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEETQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
The following BLAST results are available for this feature:
BLAST of CF831987 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 100
Match NameE-valueIdentityDescription
EF2_BETVU3.979e-8690.59Elongation factor 2 OS=Beta vulgaris PE=2 SV=1[more]
EF2_CHLKE8.316e-7679.41Elongation factor 2 OS=Chlorella kessleri PE=2 SV=... [more]
EF2_CAEEL1.627e-6368.24Elongation factor 2 OS=Caenorhabditis elegans GN=e... [more]
EF2_RAT3.624e-6367.06Elongation factor 2 OS=Rattus norvegicus GN=Eef2 P... [more]
EF2_PONAB3.624e-6367.06Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 S... [more]
EF2_MOUSE3.624e-6367.06Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 S... [more]
EF2_MESAU3.624e-6367.06Elongation factor 2 OS=Mesocricetus auratus GN=EEF... [more]
EF2_HUMAN3.624e-6367.06Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 S... [more]
EF2_CALJA6.181e-6367.06Elongation factor 2 OS=Callithrix jacchus GN=EEF2 ... [more]
EF2_BOVIN6.181e-6367.06Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01C02_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01C02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF831987 ID=CF831987; Name=CF831987; organism=Citrus sinensis; type=EST; length=878bp
GGGCCCCTCTGTTCTAATATAATAATAATATATCAACCAACGTCGGCCAG
AATTAGTCCCTACACATGACAGAACATCAAACCTTAAAAGCTGTGAATTG
TAAAATTTAAAACCTAGATGACAGAGACAGGGTTACTAAACCCAATCAAC
AGTAATATTACTTTACTAAACACAATGAGGATATTGAAAAACTTTGAAAC
AAAAATAAGCATCAGCAAAAAATATTGCTGCGCAATCTCACCCTTGATGT
CAAGATGAAATGTTTACAGCTCGTCCTCTACTCGGATAGCGGTGTCATTT
GCTCCCTCTTCCTGATATCAGCAACAAGCTGCGCAATCTCACCCTTGATG
TCAAGACGAAATGTTTACAGCTTGTCCTCAAACTCAGATAGTGGTGTCAT
TTGCTCCTTCAAGCCCTTCCTCTTCCTGATATCAGCAACAAGCTGTGCAG
CCTGTGTACCAGGCTCCAGTGGATCGGATGACATCATGTCCCAGTGATCA
AACACACATTGTGGGAAGGCCTGTCCTGACGTTGCAGCCCTCAAAGTGCC
TGAGAATCCAAAGGACTCGATGACGGGAAGGTAAGCCTTGATGTTGTAAA
GGGGGGTACCAGGTCTCTGCATTTCCTCGAACACGTGACCACGTTTCTGA
TTGAGAACACTGTAAATACCACCAAGGGCCTGCTCGGGAGCCTGTATTTC
GACCATGTAGACAGGCTCAAGAAGCCTGGGCTTGGCTGTGAGCTGGGAAG
CATATATGACCCTCCTAGCAGTAGGAATGACTTGACCACCACCTCTGTGA
ATGGCATCAGCATGAAGAACAACATCACATACTTCAAAGCAGATACCTCT
CATGTTCTCTTCAGCCAGCGCACCTTCT
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