CF832402

Overview
NameCF832402
Unique NameCF832402
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length870
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9906 contig Csv1_Contig9906:629..1498. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: COMT1_PRUDU (Caffeic acid 3-O-methyltransferase OS=Prunus dulcis GN=COMT1 PE=2 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 1.889e-64
Identity = 114/220 (51.82%), Postives = 156/220 (70.91%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD +LEG + +NKA GM  ++Y GTD RFN+VFN GM   ++  + KI+E+YKGF+ +T +VDV           +SKYP IKGINFD+PHVI+DA  YPG+EHVGGDM+  +P+GDAI MKW+ H++ DE+C+K L+NCY ALP++GKV++    LP  PDS+ A++    +D I L   P G+ERT++EF ALA  AGF+G N  C+  N+YV+EF K
Sbjct:  144 KDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMADHSTITMKKILETYKGFEGLTSVVDVGGGTGAVLNMIVSKYPSIKGINFDLPHVIEDAPQYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDSSLATKGVVHIDVIMLAHNPGGKERTEQEFQALAKGAGFQGFNVACSAFNTYVIEFLK 363
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: OMT2_CHRAE (Quercetin 3-O-methyltransferase 2 OS=Chrysosplenium americanum GN=OMT2 PE=1 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 5.495e-64
Identity = 112/220 (50.91%), Postives = 156/220 (70.91%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD +L+G + +NKA GM  ++Y GTD RFN+VFN GM   ++  + K+ ++Y+GFQ +T LVDV           +SKYP I+ INFD+PHVI+DA  YPG+EHVGGDM+  +P+GDAI MKW+ H++ DE+C+K+L+NCY ALPN+GKV++    LPEVPDS+ A++    +D I +   P G+ERT+KEF ALA  AGF+G    CN  N+Y++EF K
Sbjct:  120 KDAVLDGGIPFNKAYGMSSFEYHGTDPRFNKVFNRGMSDHSTITMKKVFQTYQGFQGLTSLVDVGGGTGATLTMILSKYPTIRCINFDLPHVIEDAPEYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKLLKNCYDALPNNGKVILAECILPEVPDSSLATKGVVHIDVITVAHNPGGKERTEKEFEALAKAAGFQGFQVFCNAFNTYIIEFSK 339
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: OMT1_CHRAE (Quercetin 3-O-methyltransferase 1 OS=Chrysosplenium americanum GN=OMT1 PE=1 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 5.495e-64
Identity = 112/220 (50.91%), Postives = 156/220 (70.91%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD +L+G + +NKA GM  ++Y GTD RFN+VFN GM   ++  + K+ ++Y+GFQ +T LVDV           +SKYP I+ INFD+PHVI+DA  YPG+EHVGGDM+  +P+GDAI MKW+ H++ DE+C+K+L+NCY ALPN+GKV++    LPEVPDS+ A++    +D I +   P G+ERT+KEF ALA  AGF+G    CN  N+Y++EF K
Sbjct:  120 KDAVLDGGIPFNKAYGMSSFEYHGTDPRFNKVFNRGMSDHSTITMKKVFQAYQGFQGLTSLVDVGGGTGATLTMILSKYPTIRCINFDLPHVIEDAPEYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKLLKNCYDALPNNGKVILAECILPEVPDSSLATKGVVHIDVITVAHNPGGKERTEKEFEALAKAAGFQGFQVFCNAFNTYIIEFSK 339
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: COMT1_ROSCH (Caffeic acid 3-O-methyltransferase OS=Rosa chinensis GN=COMT1 PE=2 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 7.177e-64
Identity = 111/220 (50.45%), Postives = 156/220 (70.91%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD +L+G + +NKA GM  +DY GTD RFN+VFN GM   ++  + KI+E+YKGF+ +T +VDV           +SKYP IKGINFD+PHVI+DA  YPG++HVGGDM+  +P+GDAI MKW+ H++ DE+C+K L+NCY ALP++GKV++    LP  PD++ A++    +D + L   P G+ERT++EF ALA  +GF+GI   CN  N+YV+EF K
Sbjct:  144 KDAVLDGGIPFNKAYGMTAFDYHGTDPRFNKVFNKGMADHSTITMKKILETYKGFEGLTSIVDVGGGTGAVVNMIVSKYPSIKGINFDLPHVIEDAPQYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDTSLATKGVVHIDVVMLAHNPGGKERTEQEFEALAKGSGFQGIRVACNAFNTYVIEFLK 363
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: OMT1_ARATH (Quercetin 3-O-methyltransferase 1 OS=Arabidopsis thaliana GN=OMT1 PE=1 SV=1)

HSP 1 Score: 243.817 bits (621), Expect = 9.374e-64
Identity = 112/220 (50.91%), Postives = 156/220 (70.91%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD IL+G + +NKA GM  ++Y GTD RFN+VFNNGM   ++  + KI+E+YKGF+ +T LVDV           +SKYP++KGINFD+PHVI+DA S+PG+EHVGGDM+  +P+GDAI MKW+ H++ DE+C+K L+NCY++LP DGKV++    LPE PDS+ +++    +D I L   P G+ERT+KEF ALA  +GFKGI   C+     ++E  K
Sbjct:  142 KDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLK 361
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: COMT1_MEDSA (Caffeic acid 3-O-methyltransferase OS=Medicago sativa PE=1 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.224e-63
Identity = 113/220 (51.36%), Postives = 153/220 (69.55%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD +L+G + +NKA GM  ++Y GTD RFN+VFN GM   ++  + KI+E+Y GF+ +  LVDV           +SKYP IKGINFD+PHVI+DA SYPG+EHVGGDM+  IP+ DA+ MKW+ H++ DE+C+K L+NCY+ALP++GKV+V    LP  PDS+ A++    +D I L   P G+ERT+KEF  LA  AGF+G    CN  N+Y+MEF K
Sbjct:  144 KDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILETYTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVVHIDVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFLK 363
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: COMT1_CLABR (Caffeic acid 3-O-methyltransferase OS=Clarkia breweri GN=COMT PE=1 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 1.036e-62
Identity = 115/220 (52.27%), Postives = 149/220 (67.73%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD IL+G + +NKA GM  ++Y GTD RFN+VFN GM   ++  + KI E Y GF+ +  +VDV           ++KYP IKGINFD+PHVI+DA  YPG+EHVGGDM+  +P+GDAI MKW+ H++ DE+C+K L+NCY ALP  GKV+V    LP  PD + A++    +DAI L   P G+ERT+KEF ALAI AGFKG    C   N+YVMEF K
Sbjct:  149 KDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMKKIFEMYTGFEALNTIVDVGGGTGAVLSMIVAKYPSIKGINFDLPHVIEDAPIYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPEHGKVIVAECILPLSPDPSLATKGVIHIDAIMLAHNPGGKERTEKEFEALAIGAGFKGFKVACCAFNTYVMEFLK 368
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: COMT1_ZINEL (Caffeic acid 3-O-methyltransferase OS=Zinnia elegans PE=2 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 6.718e-62
Identity = 110/220 (50.00%), Postives = 152/220 (69.09%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD +L+G + +NKA GM  ++Y G D RFN+VFN+GM   ++  + KIVE Y GF  +  LVDV            SK+  +KGINFD+PHVI DA++Y G+EHVGGDM++ +P+GDAI MKW+LH++ D +C+++L+NCYK+LP +GKV+V    LPE PD+T A+++   +D I L   P G+ERT+KEF ALA  AGFKG N      N++VMEF K
Sbjct:  135 KDPVLDGGIPFNKAYGMSAFEYHGKDQRFNKVFNSGMFNHSTMTMKKIVELYNGFSGLKTLVDVGGGTGASLNMITSKHKSLKGINFDLPHVIADATTYQGIEHVGGDMFESVPKGDAIFMKWILHDWSDAHCLQVLKNCYKSLPENGKVIVAECILPEAPDTTPATQNVIHIDVIMLAHNPGGKERTEKEFEALAKGAGFKGFNKAACALNTWVMEFCK 354
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: COMT1_POPKI (Caffeic acid 3-O-methyltransferase 1 OS=Populus kitakamiensis GN=HOMT1 PE=3 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 6.718e-62
Identity = 111/220 (50.45%), Postives = 151/220 (68.64%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD ILEG + +NKA GM  ++Y GTD RFN+VFN GM   ++  + KI+E+YKGF+ +T LVDV           +SKYP IKGINFD+PHVI+DA SYPG+EHVGGDM+  +P  DA+ MKW+ H++ D +C+K L+NCY ALP +GKV+++   LP  PD++ A++    +D I L   P G+ERT+KEF  LA  AGF+G    C   N++V+EF K
Sbjct:  144 KDAILEGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPNADAVFMKWICHDWSDAHCLKFLKNCYDALPENGKVILVECILPVAPDTSLATKGVVHVDVIMLAHNPGGKERTEKEFEGLANGAGFQGFEVMCCAFNTHVIEFRK 363
[Close]
BLAST of CF832402 vs. ExPASy Swiss-Prot
Match: COMT1_POPTM (Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides GN=OMT1 PE=1 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 1.146e-61
Identity = 110/220 (50.00%), Postives = 152/220 (69.09%), Query Frame = -3 
Query:  209 KDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVXXXXXXXXXXXISKYPHIKGINFDVPHVIKDASSYPGMEHVGGDMYQKIPEGDAILMKWMLHNFDDENCIKILRNCYKALPNDGKVLVINSTLPEVPDSTEASRDSFILDAIFLIQIPHGRERTKKEFTALAIEAGFKGINFECNVCNSYVMEFYK 868
            KD IL+G + +NKA GM  ++Y GTD RFN+VFN GM   ++  + KI+E+YKGF+ +T LVDV           +SKYP IKGINFD+PHVI+DA SYPG+EHVGGDM+  +P+ DA+ MKW+ H++ D +C+K L+NCY ALP +GKV+++   LP  PD++ A++    +D I L   P G+ERT+KEF  LA  AGF+G    C   N++V+EF K
Sbjct:  144 KDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAVFMKWICHDWSDAHCLKFLKNCYDALPENGKVILVECILPVAPDTSLATKGVVHVDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMCCAFNTHVIEFRK 363
The following BLAST results are available for this feature:
BLAST of CF832402 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 43
Match NameE-valueIdentityDescription
COMT1_PRUDU1.889e-6451.82Caffeic acid 3-O-methyltransferase OS=Prunus dulci... [more]
OMT2_CHRAE5.495e-6450.91Quercetin 3-O-methyltransferase 2 OS=Chrysospleniu... [more]
OMT1_CHRAE5.495e-6450.91Quercetin 3-O-methyltransferase 1 OS=Chrysospleniu... [more]
COMT1_ROSCH7.177e-6450.45Caffeic acid 3-O-methyltransferase OS=Rosa chinens... [more]
OMT1_ARATH9.374e-6450.91Quercetin 3-O-methyltransferase 1 OS=Arabidopsis t... [more]
COMT1_MEDSA1.224e-6351.36Caffeic acid 3-O-methyltransferase OS=Medicago sat... [more]
COMT1_CLABR1.036e-6252.27Caffeic acid 3-O-methyltransferase OS=Clarkia brew... [more]
COMT1_ZINEL6.718e-6250.00Caffeic acid 3-O-methyltransferase OS=Zinnia elega... [more]
COMT1_POPKI6.718e-6250.45Caffeic acid 3-O-methyltransferase 1 OS=Populus ki... [more]
COMT1_POPTM1.146e-6150.00Caffeic acid 3-O-methyltransferase 1 OS=Populus tr... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01M22_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01M22, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832402 ID=CF832402; Name=CF832402; organism=Citrus sinensis; type=EST; length=870bp
TTTTTTTTTTTTTTTTTATACCAAACACAAACAGGACTTATATCATTGAA
CACACAAAAGCATTACATAAGAAGTTCCCGCGAGCCATAAAGGCCAAACA
AAACAGAAAGTTACAGAAAAGAAAATGGAGTATCGACACTCCAAATTAAA
ATTGAGAAGATGCATCTTTCTGATAAAGAAAGAATGAAGCAATCTAGCTT
CAAAGTTACTTGTAGAATTCCATGACATAGGAATTGCAAACGTTACATTC
AAAGTTGATACCCTTAAATCCAGCTTCGATTGCCAATGCAGTGAACTCCT
TTTTGGTCCGCTCCCTTCCATGAGGAATTTGGATTAAAAAAATTGCATCT
AAAATGAAGGAATCCCTTGAAGCCTCGGTACTGTCAGGCACTTCTGGCAA
CGTTGAGTTAATAACAAGTACCTTTCCATCATTTGGAAGAGCTTTGTAAC
AATTCCTCAGTATCTTTATGCAATTCTCATCATCAAAATTATGAAGCATC
CACTTCATTAAAATGGCATCCCCCTCTGGAATTTTTTGGTACATATCTCC
GCCAACATGTTCCATGCCGGGATAAGATGAGGCATCTTTTATAACATGAG
GTACGTCAAAATTAATACCCTTAATATGAGGGTATTTGGAAATTATGGTC
GCAAGGGTGATTCCTAAACCACCGCCAACGTCAACAAGGCGCGTAATATT
CTGAAAGCCTTTGTAGGATTCTACAATCTTATTGATTACAAAAGAAGTCT
GGCCGATCATTCCATTGTTGAAAACTTCATTGAACCTGGAGTCGGTGCCC
AGATAATCGTAGAAATCCATTCCGAGGGCTTTATTATACGAAAGTTCTCC
TTCAAGAATTTGGTCTTTCA
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