CF834868

Overview
NameCF834868
Unique NameCF834868
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length779
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2387 contig Csv1_Contig2387:233..1012. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_PEA (Pyrroline-5-carboxylate reductase OS=Pisum sativum GN=PROC PE=2 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 1.372e-84
Identity = 87/97 (89.69%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            L GSGPAYI+LAIEALADGGVAAGLPR+LAL LASQTVLGAASM T SGKHPGQLKDDV SPGGTTIAG+HELEK GFRG LMNAVVAAAKRSRELS
Sbjct:  177 LSGSGPAYIYLAIEALADGGVAAGLPRDLALSLASQTVLGAASMATLSGKHPGQLKDDVTSPGGTTIAGVHELEKGGFRGTLMNAVVAAAKRSRELS 273

HSP 2 Score: 164.466 bits (415), Expect = 1.372e-84
Identity = 83/135 (61.48%), Postives = 99/135 (73.33%), Query Frame = 2 
Query:   11 ALSNLKRRDAFESIGVKVLSDNNAXXXXXXXXXXXXXXXXXXXXAMQIRPLLSRKKLLVSVAAGVKLKDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            A SN  RR AFESIG+ VLS N+                      ++++PLL++ KLLVSVAAG+KLKDLQEW GH RFIRVMPNTP+AVG+AA+VMSLGG ATEED  LI +LFGS+GKIW+AD+K FDAITGL
Sbjct:   43 AHSNPSRRAAFESIGITVLSSNDDVVRASNVVVFSVKPQLVKDVVLKLKPLLTKDKLLVSVAAGIKLKDLQEWAGHERFIRVMPNTPAAVGQAASVMSLGGAATEEDANLISQLFGSIGKIWKADDKFFDAITGL 177
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_ACTCH (Pyrroline-5-carboxylate reductase OS=Actinidia chinensis PE=2 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 3.971e-84
Identity = 85/96 (88.54%), Postives = 89/96 (92.71%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            L GSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMV + GKHPGQLKDDVAS GGTTIAGIHELEK GFRG LMNAVV+A KRS+E+
Sbjct:  180 LSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVARGGKHPGQLKDDVASAGGTTIAGIHELEKGGFRGTLMNAVVSATKRSQEI 275

HSP 2 Score: 166.392 bits (420), Expect = 3.971e-84
Identity = 83/129 (64.34%), Postives = 98/129 (75.97%), Query Frame = 2 
Query:   29 RRDAFESIGVKVLSDNNAXXXXXXXXXXXXXXXXXXXXAMQIRPLLSRKKLLVSVAAGVKLKDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            RR+AFES GVKVL  N+                      +Q+RPLLS K+LLVS+ AGVKLK+L++W GHSRFIRVMPNTP+AVGEAA+VMSLG TAT EDGELI KLFG++GKIW+ADEKLFDA+TGL
Sbjct:   52 RREAFESFGVKVLDRNDQVVEDSDVIIFSVKPQIVKEVVLQLRPLLSEKQLLVSIVAGVKLKELEDWAGHSRFIRVMPNTPAAVGEAASVMSLGATATGEDGELITKLFGAIGKIWKADEKLFDAVTGL 180
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_SOYBN (Pyrroline-5-carboxylate reductase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 1.792e-81
Identity = 83/97 (85.57%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            L GSGPAY++LAIEALADGGVAAGLPR+L+L LASQTVLGAASMV+++GKHPGQLKDDV SPGGTTI GIHELE  GFRG LMNAVVAAAKRSRELS
Sbjct:  178 LSGSGPAYVYLAIEALADGGVAAGLPRDLSLSLASQTVLGAASMVSQTGKHPGQLKDDVTSPGGTTITGIHELENGGFRGTLMNAVVAAAKRSRELS 274

HSP 2 Score: 158.688 bits (400), Expect = 1.792e-81
Identity = 82/137 (59.85%), Postives = 97/137 (70.80%), Query Frame = 2 
Query:    5 TAALSNLKRRDAFESIGVKVLSDNNAXXXXXXXXXXXXXXXXXXXXAMQIRPLLSRKKLLVSVAAGVKLKDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            TA   NL RR AFES GV VL  N+                       ++ PLL++ KLLVSVAAG KLKDLQEW G+ RFIRVMPNTP+AVG+AA+VMSLGG+ATEEDG +I +LFGS+GKIW+A+EK FDAITGL
Sbjct:   42 TAVHFNLARRGAFESFGVTVLPSNDDVVRESDVVVLSVKPQLVKDVVSKLTPLLTKHKLLVSVAAGTKLKDLQEWAGNDRFIRVMPNTPAAVGQAASVMSLGGSATEEDGNIIAQLFGSIGKIWKAEEKYFDAITGL 178
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR1_ARATH (Pyrroline-5-carboxylate reductase OS=Arabidopsis thaliana GN=PROC1 PE=2 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 1.258e-79
Identity = 85/97 (87.63%), Postives = 89/97 (91.75%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            L GSGPAYIFLAIEALADGGVAAGLPRELAL LASQTVLGAA+MV+K+GKHPG LKDDV SPGGTTIAG+HELEK  FR  LMNAVVAAAKRSRELS
Sbjct:  178 LSGSGPAYIFLAIEALADGGVAAGLPRELALSLASQTVLGAATMVSKTGKHPGVLKDDVTSPGGTTIAGVHELEKGSFRATLMNAVVAAAKRSRELS 274

HSP 2 Score: 152.525 bits (384), Expect = 1.258e-79
Identity = 78/138 (56.52%), Postives = 94/138 (68.12%), Query Frame = 2 
Query:    2 CTAALSNLKRRDAFESIGVKVLSDNNAXXXXXXXXXXXXXXXXXXXXAMQIRPLLSRKKLLVSVAAGVKLKDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            CTA  SNL RRD FES GV V S +                        +++  LS+ K+LVSVAAG+KL DLQEW+G  RFIRVMPNTP+AVGEAA+VMSLG  ATEEDG ++  LFG+VGKI +ADEK+FDA+TGL
Sbjct:   41 CTAVHSNLNRRDVFESFGVNVFSTSEEVVKESDVVIFSVKPQVVKKAVTELKSKLSKNKILVSVAAGIKLNDLQEWSGQDRFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKILKADEKMFDAVTGL 178
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_METAC (Pyrroline-5-carboxylate reductase OS=Methanosarcina acetivorans GN=proC PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.827e-38
Identity = 59/96 (61.46%), Postives = 73/96 (76.04%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            L GSGPA+IF  IEA+ADG V  G+ R+ AL LA+QTVLGAA M  ++G HPG+LKD V SP GTTI GIH LE++G R   MNAV+ A++RS+EL
Sbjct:  172 LSGSGPAFIFPVIEAMADGAVLEGMDRKSALTLAAQTVLGAAKMALETGMHPGELKDMVTSPAGTTIQGIHSLEEAGIRAAFMNAVIRASERSKEL 267

HSP 2 Score: 61.2326 bits (147), Expect = 2.827e-38
Identity = 38/129 (29.46%), Postives = 65/129 (50.39%), Query Frame = 2 
Query:   32 RDAFESIGVKVLSDNNAXXXXXXXXXXXXXXXXXXXXAMQIRPLLSRKKLLVSVAAGVKLKDLQE-WTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            +D    +G++V +DN                         ++  ++ +KL++S+AAGV L   ++     +R +RVMPN  + V EAA+ ++ G  AT ED +   ++F +VG   +  E L DA+TGL
Sbjct:   44 KDLQAKLGIRVSTDNAVIVRESDILILAVKPQTLSSVLSNLKNEITSEKLVISIAAGVPLSTYEDALLEGTRVVRVMPNIAATVSEAASGIAPGKNATPEDLKAALEIFSAVGTAVQVPESLMDAVTGL 172
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR2_BOVIN (Pyrroline-5-carboxylate reductase 2 OS=Bos taurus GN=PYCR2 PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 8.121e-38
Identity = 59/96 (61.46%), Postives = 73/96 (76.04%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            L GSGPAY F+A++ALADGGV  GLPR LA+ L +Q +LGAA M+  S +HPGQLKD+V SPGG TI  +H LE  GFR +L+NAV A+  R+REL
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTREL 268

HSP 2 Score: 58.9214 bits (141), Expect = 8.121e-38
Identity = 36/85 (42.35%), Postives = 48/85 (56.47%), Query Frame = 2 
Query:  173 KKLLVSVAAGVKL----KDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            + ++VS AAGV +    K L  +    + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DA+TGL
Sbjct:   89 RHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVLVREGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR1_PONAB (Pyrroline-5-carboxylate reductase 1, mitochondrial OS=Pongo abelii GN=PYCR1 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 8.122e-38
Identity = 59/96 (61.46%), Postives = 73/96 (76.04%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            L GSGPAY F A++ALADGGV  GLPR LA+ L +Q +LGAA M+  S +HPGQLKD+V+SPGG TI  +H LE  GFR +L+NAV A+  R+REL
Sbjct:  173 LSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTREL 268

HSP 2 Score: 59.3066 bits (142), Expect = 8.122e-38
Identity = 37/85 (43.53%), Postives = 47/85 (55.29%), Query Frame = 2 
Query:  173 KKLLVSVAAGVKL----KDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            + ++VS AAGV +    K L  +    R IR M NTP  V E ATV + G  A  EDG L+ +L  SVG     +E L DA+TGL
Sbjct:   89 RHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR1_HUMAN (Pyrroline-5-carboxylate reductase 1, mitochondrial OS=Homo sapiens GN=PYCR1 PE=1 SV=2)

HSP 1 Score: 119.013 bits (297), Expect = 8.122e-38
Identity = 59/96 (61.46%), Postives = 73/96 (76.04%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            L GSGPAY F A++ALADGGV  GLPR LA+ L +Q +LGAA M+  S +HPGQLKD+V+SPGG TI  +H LE  GFR +L+NAV A+  R+REL
Sbjct:  173 LSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTREL 268

HSP 2 Score: 59.3066 bits (142), Expect = 8.122e-38
Identity = 37/85 (43.53%), Postives = 47/85 (55.29%), Query Frame = 2 
Query:  173 KKLLVSVAAGVKL----KDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            + ++VS AAGV +    K L  +    R IR M NTP  V E ATV + G  A  EDG L+ +L  SVG     +E L DA+TGL
Sbjct:   89 RHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR2_RAT (Pyrroline-5-carboxylate reductase 2 OS=Rattus norvegicus GN=Pycr2 PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 1.795e-37
Identity = 58/96 (60.42%), Postives = 72/96 (75.00%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            L GSGPAY F+A++ALADGGV  G+PR LA+ L +Q +LGAA M+  S  HPGQLKD+V SPGG TI  +H LE  GFR +L+NAV A+  R+REL
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQALLGAAKMLLDSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTREL 268

HSP 2 Score: 59.6918 bits (143), Expect = 1.795e-37
Identity = 37/88 (42.05%), Postives = 49/88 (55.68%), Query Frame = 2 
Query:  164 LSRKKLLVSVAAGVKL----KDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            +  + ++VS AAGV +    K L  +    + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DAITGL
Sbjct:   86 VQERHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVREGATVYATGTHALVEDGKLLEQLMSSVGFCTEVEEDLIDAITGL 173
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR2_MOUSE (Pyrroline-5-carboxylate reductase 2 OS=Mus musculus GN=Pycr2 PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 1.795e-37
Identity = 58/96 (60.42%), Postives = 72/96 (75.00%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            L GSGPAY F+A++ALADGGV  G+PR LA+ L +Q +LGAA M+  S  HPGQLKD+V SPGG TI  +H LE  GFR +L+NAV A+  R+REL
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQALLGAAKMLLDSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTREL 268

HSP 2 Score: 59.6918 bits (143), Expect = 1.795e-37
Identity = 37/88 (42.05%), Postives = 49/88 (55.68%), Query Frame = 2 
Query:  164 LSRKKLLVSVAAGVKL----KDLQEWTGHSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            +  + ++VS AAGV +    K L  +    + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DAITGL
Sbjct:   86 VQERHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVREGATVYATGTHALVEDGKLLEQLMSSVGFCTEVEEDLIDAITGL 173
The following BLAST results are available for this feature:
BLAST of CF834868 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 53
Match NameE-valueIdentityDescription
P5CR_PEA1.372e-8489.69Pyrroline-5-carboxylate reductase OS=Pisum sativum... [more]
P5CR_ACTCH3.971e-8488.54Pyrroline-5-carboxylate reductase OS=Actinidia chi... [more]
P5CR_SOYBN1.792e-8185.57Pyrroline-5-carboxylate reductase OS=Glycine max P... [more]
P5CR1_ARATH1.258e-7987.63Pyrroline-5-carboxylate reductase OS=Arabidopsis t... [more]
P5CR_METAC2.827e-3861.46Pyrroline-5-carboxylate reductase OS=Methanosarcin... [more]
P5CR2_BOVIN8.121e-3861.46Pyrroline-5-carboxylate reductase 2 OS=Bos taurus ... [more]
P5CR1_PONAB8.122e-3861.46Pyrroline-5-carboxylate reductase 1, mitochondrial... [more]
P5CR1_HUMAN8.122e-3861.46Pyrroline-5-carboxylate reductase 1, mitochondrial... [more]
P5CR2_RAT1.795e-3760.42Pyrroline-5-carboxylate reductase 2 OS=Rattus norv... [more]
P5CR2_MOUSE1.795e-3760.42Pyrroline-5-carboxylate reductase 2 OS=Mus musculu... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS02_06E05_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06E05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834868 ID=CF834868; Name=CF834868; organism=Citrus sinensis; type=EST; length=779bp
CTGCACCGCCGCCCTCTCTAATCTCAAACGCCGTGATGCTTTTGAGTCCA
TCGGCGTCAAAGTATTATCCGACAATAACGCCGTTGTTGAATACAGCGAT
GTGGTTGTTTTCTCTGTGAAACCGCAAGTAGTTAAAGATGTGGCCATGCA
GATAAGGCCGCTGTTATCACGGAAGAAGCTTTTGGTTTCTGTTGCTGCAG
GAGTGAAATTGAAAGACCTTCAGGAATGGACTGGTCACAGCCGGTTTATT
AGAGTTATGCCTAATACACCTTCTGCTGTAGGTGAGGCTGCAACAGTTAT
GAGCTTGGGAGGAACTGCAACAGAAGAGGATGGGGAACTAATCGGTAAAT
TATTCGGATCAGTTGGCAAGATATGGCGAGCGGATGAGAAATTGTTTGAT
GCAATCACGGGCCTGAGGTATGTGGCTTCTTGGCAGCGGTCCAGCATATA
TATTTTTAGCAATTGAAGCTTTGGCTGATGGAGGTGTGGCTGCAGGGCTA
CCGCGAGAACTTGCACTTGGTCTAGCTTCTCAGACAGTATTAGGAGCAGC
ATCAATGGTCACTAAAAGTGGGAAGCATCCTGGTCAGCTAAAAGATGATG
TTGCATCGCCTGGTGGGACTACTATTGCTGGTATTCATGAGTTGGAGAAA
AGTGGATTTCGTGGGATATTAATGAATGCTGTTGTTGCTGCTGCTAAGCG
AAGCCGAGAACTGTCCTAGAGCTAATTCATTTTGATTCCAGTTTCTCAGC
TCTTACACTTCTAGTATGGCGATTAAACC
back to top