CF835073

Overview
NameCF835073
Unique NameCF835073
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA4_HUMAN (Protein disulfide-isomerase A4 OS=Homo sapiens GN=PDIA4 PE=1 SV=2)

HSP 1 Score: 133.265 bits (334), Expect = 1.264e-30
Identity = 74/193 (38.34%), Postives = 113/193 (58.55%), Query Frame = -3
Query:  105 IADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESDLTPSN-IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHP--KLQVEEYPTLLFYPAGDKANPIKVSARSSS-KNIAAFIKEQLKEKDQSPKD 671
            IADE+     +   GL ES   V  A  +++  KF +E +   S+ + EF +    G L P +KSQP+P N    V++VVGKTFD +V++  KDVL+E Y PWC  C+        LAK +KG   LVIAK+DA+AN+ P  + +VE +PT+ F P+GDK NP+K        ++++ FI+E   +  ++ ++
Sbjct:  452 IADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEE 644          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA4_RAT (Protein disulfide-isomerase A4 OS=Rattus norvegicus GN=Pdia4 PE=1 SV=2)

HSP 1 Score: 131.339 bits (329), Expect = 4.803e-30
Identity = 73/193 (37.82%), Postives = 114/193 (59.07%), Query Frame = -3
Query:  105 IADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESDLTPSN-IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANE--HPKLQVEEYPTLLFYPAGDKANPIKVSARSSS-KNIAAFIKEQLKEKDQSPKD 671
            IADE+     +   GL ES   V  A  +++  KF +E +   S+ ++EF      G L P +KSQP+P N    V++VVGKTFD +V++  KDVL+E Y PWC  C+        L K +KG  +LVIAK+DA+AN+  + + +VE +PT+ F P+GDK NPIK    +   ++++ FI E   ++ ++ ++
Sbjct:  450 IADEEDYATEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDALQEFVMAFKKGKLKPVIKSQPVPKNNKGPVRVVVGKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPVYTSLGKKYKGQKDLVIAKMDATANDITNDRYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFIDEHATKRSRTKEE 642          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA4_MOUSE (Protein disulfide-isomerase A4 OS=Mus musculus GN=Pdia4 PE=1 SV=2)

HSP 1 Score: 130.568 bits (327), Expect = 8.192e-30
Identity = 73/193 (37.82%), Postives = 114/193 (59.07%), Query Frame = -3
Query:  105 IADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESDLTPSN-IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANE--HPKLQVEEYPTLLFYPAGDKANPIKVSARSSS-KNIAAFIKEQLKEKDQSPKD 671
            IADE+     +   GL ES   V  A  +++  KF +E +   S+ + EF +    G L P +KSQP+P N    V++VVGKTFD +V++  KDVL+E Y PWC  C+        L K +KG  +LVIAK+DA+AN+  + + +VE +PT+ F P+GDK NPIK    +   ++++ FI E   ++ ++ ++
Sbjct:  445 IADEEDYATEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPIYTSLGKKYKGQKDLVIAKMDATANDITNDQYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFIDEHATKRSRTKEE 637          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA4_BOVIN (Protein disulfide-isomerase A4 OS=Bos taurus GN=PDIA4 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 5.310e-29
Identity = 71/193 (36.79%), Postives = 108/193 (55.96%), Query Frame = -3
Query:  105 IADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLE-SDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANE--HPKLQVEEYPTLLFYPAGDKANPIKV-SARSSSKNIAAFIKEQLKEKDQSPKD 671
            +ADE+     L   GL ES   V  A  ++   +F +E  D     + +F +    G L P +KSQP+P N    V++VVGKTFD +V++  KDVL+E Y PWC  C+        L K +KG  NLVIAK+DA+AN+    + +VE +PT+ F P+GDK  PIK        ++++ FI+E   +  ++ ++
Sbjct:  450 VADEEDFATELKDLGLSESGEEVNAAILDEGGRRFAMEPDDFDADALRDFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYTSLGKKYKGHKNLVIAKMDATANDVTSDRYKVEGFPTIYFAPSGDKKKPIKFEDGNRDLEHLSKFIEEHATKLSRTKEE 642          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_CHICK (Protein disulfide-isomerase OS=Gallus gallus GN=P4HB PE=1 SV=3)

HSP 1 Score: 116.316 bits (290), Expect = 1.599e-25
Identity = 66/227 (29.07%), Postives = 125/227 (55.07%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK-------------EQLKEKDQSPKDEQLKERDQAPKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ESD LT   I+EFC++ L G + P+L SQ +P++ +   V+++VGK F+++  + +K+V +E Y PWC  C+  +   +KL + ++  +N+VIAK+D++ANE   +++  +PTL F+PAG   N I  +   + +    F++             E L+  +++  +E   +  +  KDEL
Sbjct:  290 KILFIFID-SDHSDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDDLTADKIKEFCNKFLEGKIKPHLMSQDLPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYRDHENIVIAKMDSTANEVEAVKIHSFPTLKFFPAGSGRNVIDYNGERTLEGFKKFLESGGQDGAAADDDLEDLETDEETDLEEGDDDEQKIQKDEL 515          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA3_BOVIN (Protein disulfide-isomerase A3 OS=Bos taurus GN=PDIA3 PE=2 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 6.075e-25
Identity = 66/197 (33.50%), Postives = 110/197 (55.84%), Query Frame = -3
Query:   69 LTLFGLEESKNTV-VTAFDNKAISKFLLESDLTPSN--IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSP--KDEQLKERDQAPKD 641
            L+ FGLE +   + V A       KF+++ + +     +E F      G L  YLKS+PIP++ +  V++VV + FD++V N +KDVL+E Y PWC  C+    + ++L +  +   N+VIAK+DA+AN+ P   +V  +PT+ F PA  K NP K       + ++ FI    +E    P  ++E+ K++ +A +D
Sbjct:  311 LSDFGLESTTGEIPVVAVRTAKGEKFVMQEEFSRDGKALERFLEDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLRKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKQNPKKY---EGGRELSDFISYLKREATNPPVIQEEKPKKKKKAQED 504          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA3_PONAB (Protein disulfide-isomerase A3 OS=Pongo abelii GN=PDIA3 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 7.935e-25
Identity = 66/197 (33.50%), Postives = 109/197 (55.33%), Query Frame = -3
Query:   69 LTLFGLEESKNTV-VTAFDNKAISKFLLESDLTPSN--IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSP--KDEQLKERDQAPKD 641
            L+ FGLE +   + V A       KF+++ + +     +E F      G L  YLKS+PIP++ +  V++VV + FD++V N +KDVL+E Y PWC  C+    + ++L +      N+VIAK+DA+AN+ P   +V  +PT+ F PA  K NP K       + ++ FI    +E    P  ++E+ K++ +A +D
Sbjct:  311 LSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKY---EGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQED 504          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA3_HUMAN (Protein disulfide-isomerase A3 OS=Homo sapiens GN=PDIA3 PE=1 SV=4)

HSP 1 Score: 114.005 bits (284), Expect = 7.935e-25
Identity = 66/197 (33.50%), Postives = 109/197 (55.33%), Query Frame = -3
Query:   69 LTLFGLEESKNTV-VTAFDNKAISKFLLESDLTPSN--IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSP--KDEQLKERDQAPKD 641
            L+ FGLE +   + V A       KF+++ + +     +E F      G L  YLKS+PIP++ +  V++VV + FD++V N +KDVL+E Y PWC  C+    + ++L +      N+VIAK+DA+AN+ P   +V  +PT+ F PA  K NP K       + ++ FI    +E    P  ++E+ K++ +A +D
Sbjct:  311 LSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKY---EGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQED 504          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA3_CERAE (Protein disulfide-isomerase A3 OS=Cercopithecus aethiops GN=PDIA3 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 7.935e-25
Identity = 66/197 (33.50%), Postives = 109/197 (55.33%), Query Frame = -3
Query:   69 LTLFGLEESKNTV-VTAFDNKAISKFLLESDLTPSN--IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSP--KDEQLKERDQAPKD 641
            L+ FGLE +   + V A       KF+++ + +     +E F      G L  YLKS+PIP++ +  V++VV + FD++V N +KDVL+E Y PWC  C+    + ++L +      N+VIAK+DA+AN+ P   +V  +PT+ F PA  K NP K       + ++ FI    +E    P  ++E+ K++ +A +D
Sbjct:  311 LSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKY---EGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQED 504          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_BOVIN (Protein disulfide-isomerase OS=Bos taurus GN=P4HB PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 7.935e-25
Identity = 66/214 (30.84%), Postives = 119/214 (55.61%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ESD LT   I EFC R L G + P+L SQ +PD+ +   V+++VGK F+++  +  K+V +E Y PWC  C+  +   +KL + +K  +N+VIAK+D++ANE   ++V  +PTL F+PA      I  +   +      F++     +D +  D+ L++ ++A + +L
Sbjct:  287 KILFIFID-SDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLES--GGQDGAGDDDDLEDLEEAEEPDL 497          
The following BLAST results are available for this feature:
BLAST of CF835073 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 51
Match NameE-valueIdentityDescription
PDIA4_HUMAN1.264e-3038.34Protein disulfide-isomerase A4 OS=Homo sapiens GN=... [more]
PDIA4_RAT4.803e-3037.82Protein disulfide-isomerase A4 OS=Rattus norvegicu... [more]
PDIA4_MOUSE8.192e-3037.82Protein disulfide-isomerase A4 OS=Mus musculus GN=... [more]
PDIA4_BOVIN5.310e-2936.79Protein disulfide-isomerase A4 OS=Bos taurus GN=PD... [more]
PDIA1_CHICK1.599e-2529.07Protein disulfide-isomerase OS=Gallus gallus GN=P4... [more]
PDIA3_BOVIN6.075e-2533.50Protein disulfide-isomerase A3 OS=Bos taurus GN=PD... [more]
PDIA3_PONAB7.935e-2533.50Protein disulfide-isomerase A3 OS=Pongo abelii GN=... [more]
PDIA3_HUMAN7.935e-2533.50Protein disulfide-isomerase A3 OS=Homo sapiens GN=... [more]
PDIA3_CERAE7.935e-2533.50Protein disulfide-isomerase A3 OS=Cercopithecus ae... [more]
PDIA1_BOVIN7.935e-2530.84Protein disulfide-isomerase OS=Bos taurus GN=P4HB ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06J24_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06J24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835073 ID=CF835073; Name=CF835073; organism=Citrus sinensis; type=EST; length=706bp
TTTTTTTTTTTTTTTCCTTTTTCTGTTAAAGTTTCAATATTCCACCCCTT
TTATAAGATCTATAATTCATCTTTGGGAGCTTGATCCCTTTCTTTCAATT
GTTCATCTTTTGGGGATTGATCTTTTTCTTTCAATTGTTCCTTGATAAAT
GCTGCTATGTTCTTTGAGCTGGACCTTGCTGAAACTTTGATCGGATTTGC
TTTATCACCAGCTGGGTAGAATAAAAGTGTTGGGTACTCTTCAACCTGCA
GTTTTGGATGCTCATTTGCAGAAGCATCTATCTTTGCAATGACTAGATTG
TCCAAACCCTTGAAGTGCTTAGCCAGCTTTTCGATTTGCTTGCTAGTTGT
CTCACATCTGACACACCATGGTGTATATACCTCTAGAAGAACATCCTTGT
GACTGTTCAAAACCAAGTCGTCAAATGTCTTTCCCACAACAATCTGCACG
TTTGCGTTTGTGTTATCTGGTATTGGCTGTGATTTCAAGTAAGGCGTCAG
AGTACCATGCAAAAGCCTTGAGCAAAACTCTTCTATGTTGCTTGGCGTGA
GATCTGACTCCAACAAAAACTTTGAAATGGCTTTGTTATCAAAGGCAGTC
ACCACAGTGTTCTTTGATTCTTCAAGCCCAAATAAAGTTAAAAATGGCTT
CGCCAGGTCTTCATCTGCGATATCTACTGCTGTAAACATGAATCTTTCCC
TTGAAA
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