EY659590 | Citrus Genome Database

Overview


Name	EY659590
Unique Name	EY659590
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	925

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig5270	contig	Csv1_Contig5270:177..1102.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: NDRA_DICDI (Probable serine/threonine-protein kinase ndrA OS=Dictyostelium discoideum GN=ndrA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.910e-34
Identity = 61/130 (46.92%), Postives = 89/130 (68.46%), Query Frame = 2

Query:  332 SEEVPSNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S +  S  T  +  AAK YIE +Y    +S++ER +RR  LE KL + ++S +E N+L K L+KKE++YMR++R K+   DFE + +IG+GAFGEV + R   +  +YAMK+LKKSEML++ Q  HV+AE
Sbjct:   31 SSDPLSRPTMDRSLAAKMYIEQYYINAQQSVKERGQRRKDLELKLENMKLSSKESNDLRKELDKKESDYMRIKRLKLKRSDFEVIRIIGRGAFGEVSLVRHRESNDLYAMKRLKKSEMLKKEQAAHVRAE 160

HSP 2 Score: 35.039 bits (79), Expect = 2.910e-34
Identity = 16/29 (55.17%), Postives = 21/29 (72.41%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LA  + + + KLY SF DD YLYLIM
Sbjct:  161 RDVLASANTNWVVKLYYSFQDDNYLYLIM 189

HSP 3 Score: 31.9574 bits (71), Expect = 2.910e-34
Identity = 13/22 (59.09%), Postives = 17/22 (77.27%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERK 872
            EYLPGG MM+LL++ D  TE +
Sbjct:  190 EYLPGGDMMSLLIKYDIFTENQ 211

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: SAX1_CAEEL (Serine/threonine-protein kinase sax-1 OS=Caenorhabditis elegans GN=sax-1 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 3.409e-31
Identity = 54/125 (43.20%), Postives = 84/125 (67.20%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S  TK K +  +  IE++Y K++    ER+ R   LE+ ++   +S+EE+    K    KET+Y+RL+R ++  +DFE L +IG+GAFGEVR+ +++ TGH+YAMK L+KSEM+ + Q  HV+AE
Sbjct:   11 SQYTKDKASCTRISIESYYSKRVTQCAERENRLKKLEEDISARGLSDEEKEEKRKIHHSKETDYLRLKRTRLTVNDFESLKVIGRGAFGEVRLVQKHDTGHIYAMKILRKSEMVEKEQTAHVRAE 135

HSP 2 Score: 36.965 bits (84), Expect = 3.409e-31
Identity = 15/20 (75.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTE 866
            E+LPGG MMTLL+++DTLTE
Sbjct:  165 EFLPGGDMMTLLIKKDTLTE 184

HSP 3 Score: 29.261 bits (64), Expect = 3.409e-31
Identity = 13/29 (44.83%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++L+E D   + K+Y SF D   LYL+M
Sbjct:  136 RDILSEADCDWVVKMYYSFQDYSNLYLVM 164

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: SAX1_CAEBR (Serine/threonine-protein kinase sax-1 OS=Caenorhabditis briggsae GN=sax-1 PE=3 SV=2)

HSP 1 Score: 110.153 bits (274), Expect = 5.751e-31
Identity = 53/125 (42.40%), Postives = 85/125 (68.00%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S  TK K +  +  IE++Y K++    ER+ R   LE+ ++   +S++E++   K    KET+Y+RL+R ++  +DFE L +IG+GAFGEVR+ +++ TGH+YAMK L+KSEM+ + Q  HV+AE
Sbjct:   11 SQYTKDKASCTRISIESYYSKRVTQCAERENRLKKLEEDMSARGLSDDEKDEKRKIHHSKETDYLRLKRTRLTVNDFESLKVIGRGAFGEVRLVQKHDTGHIYAMKILRKSEMVEKEQTAHVRAE 135

HSP 2 Score: 36.965 bits (84), Expect = 5.751e-31
Identity = 15/20 (75.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTE 866
            E+LPGG MMTLL+++DTLTE
Sbjct:  165 EFLPGGDMMTLLIKKDTLTE 184

HSP 3 Score: 29.261 bits (64), Expect = 5.751e-31
Identity = 13/29 (44.83%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++L+E D   + K+Y SF D   LYL+M
Sbjct:  136 RDILSEADCDWVVKMYYSFQDYSNLYLVM 164

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: CBK1_KLULA (Serine/threonine-protein kinase CBK1 OS=Kluyveromyces lactis GN=CBK1 PE=3 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 1.618e-30
Identity = 59/125 (47.20%), Postives = 82/125 (65.60%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            +  T+ K AA K  IEN Y+  +    ER +RR  LE +LA  + SEE +N  L  L KKE++++RL+R ++  DDF  + +IGKGAFGEVR+ ++  TG +YAMK L KSEM  + Q+ HVKAE
Sbjct:  227 TKTTQDKAAAVKLKIENFYQSSVGYAIERNQRRLELESELASQDWSEERKNRQLASLGKKESQFLRLRRTRLSLDDFNSVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYNKDQLAHVKAE 351

HSP 2 Score: 31.5722 bits (70), Expect = 1.618e-30
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LA  D   +  LY SF D +YLYLIM
Sbjct:  352 RDVLAGSDSPWVVSLYYSFQDSQYLYLIM 380

HSP 3 Score: 29.6462 bits (65), Expect = 1.618e-30
Identity = 12/30 (40.00%), Postives = 17/30 (56.67%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L+R    TE      +  C
Sbjct:  381 EFLPGGDLMTMLIRWQIFTEDVTRFYMAEC 410

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: ST38L_HUMAN (Serine/threonine-protein kinase 38-like OS=Homo sapiens GN=STK38L PE=1 SV=3)

HSP 1 Score: 114.775 bits (286), Expect = 3.096e-30
Identity = 56/125 (44.80%), Postives = 86/125 (68.80%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            SN T+++V  AK  +EN Y   +   EER+ R+  LE  + +  +++EE+        +KETE++RL+R ++G DDFE L +IG+GAFGEVR+ ++  TGH+YAMK L+KS+ML + QV H++AE
Sbjct:   14 SNHTRERVTVAKLTLENFYSNLILQHEERETRQKKLEVAMEEEGLADEEKKLRRSQHARKETEFLRLKRTRLGLDDFESLKVIGRGAFGEVRLVQKKDTGHIYAMKILRKSDMLEKEQVAHIRAE 138

HSP 2 Score: 38.891 bits (89), Expect = 3.096e-30
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERK 872
            E+LPGG MMTLLM++DTLTE +
Sbjct:  168 EFLPGGDMMTLLMKKDTLTEEE 189

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: CBK1_ASHGO (Serine/threonine-protein kinase CBK1 OS=Ashbya gossypii GN=CBK1 PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 5.971e-30
Identity = 58/128 (45.31%), Postives = 84/128 (65.62%), Query Frame = 2

Query:  338 EVPSNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            E+ S  T++K AA K  +EN Y+  +    ER +RR  LE +L     SEE +N  L  L KKE++++RL+R ++  +DF  + +IGKGAFGEVR+ ++  TG +YAMK L KSEM ++ Q+ HVKAE
Sbjct:  231 ELLSKSTQEKAAAVKLKVENFYQSSVNHAIERNQRRVELESQLLSHGWSEERKNRQLSSLGKKESQFLRLRRTRLSLEDFHTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAE 358

HSP 2 Score: 30.8018 bits (68), Expect = 5.971e-30
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LA  D   +  LY SF D +YLYLIM
Sbjct:  359 RDVLAGSDSPWVVSLYYSFQDAQYLYLIM 387

HSP 3 Score: 29.6462 bits (65), Expect = 5.971e-30
Identity = 12/30 (40.00%), Postives = 17/30 (56.67%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L+R    TE      +  C
Sbjct:  388 EFLPGGDLMTMLIRWQIFTEDVTRFYMAEC 417

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: CBK1_CANGA (Serine/threonine-protein kinase CBK1 OS=Candida glabrata GN=CBK1 PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 7.739e-30
Identity = 58/125 (46.40%), Postives = 82/125 (65.60%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S  T+ K AA K  +EN+Y++ +K   ER ERR  LE +L     SEE     L  L KKE++++RL+R ++  +DF  + +IGKGAFGEVR+ ++  TG +YAMK L KSEM ++ Q+ HVKAE
Sbjct:  294 SKSTQDKAAAVKLKVENYYQQSVKYAIERNERRVELETELGSHNWSEERNARQLASLGKKESQFLRLRRTRLSLEDFHTVQVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAE 418

HSP 2 Score: 31.187 bits (69), Expect = 7.739e-30
Identity = 16/29 (55.17%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LA  D   I  LY SF D +YLYLIM
Sbjct:  419 RDVLAGTDSPWIVSLYYSFQDAQYLYLIM 447

HSP 3 Score: 29.6462 bits (65), Expect = 7.739e-30
Identity = 12/30 (40.00%), Postives = 17/30 (56.67%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L+R    TE      +  C
Sbjct:  448 EFLPGGDLMTMLIRWQLFTEDVTRFYMAEC 477

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: CBK1_YEAST (Serine/threonine-protein kinase CBK1 OS=Saccharomyces cerevisiae GN=CBK1 PE=1 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.005e-29
Identity = 58/122 (47.54%), Postives = 81/122 (66.39%), Query Frame = 2

Query:  356 TKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            T+ K AA K  IEN Y+  +K   ER ERR  LE +L     SEE ++  L  L KKE++++RL+R ++  +DF  + +IGKGAFGEVR+ ++  TG +YAMK L KSEM ++ Q+ HVKAE
Sbjct:  279 TQDKAAAVKLKIENFYQSSVKYAIERNERRVELETELTSHNWSEERKSRQLSSLGKKESQFLRLRRTRLSLEDFHTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAE 400

HSP 2 Score: 30.8018 bits (68), Expect = 1.005e-29
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LA  D   +  LY SF D +YLYLIM
Sbjct:  401 RDVLAGSDSPWVVSLYYSFQDAQYLYLIM 429

HSP 3 Score: 29.6462 bits (65), Expect = 1.005e-29
Identity = 12/30 (40.00%), Postives = 17/30 (56.67%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L+R    TE      +  C
Sbjct:  430 EFLPGGDLMTMLIRWQLFTEDVTRFYMAEC 459

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: STK38_PONAB (Serine/threonine-protein kinase 38 OS=Pongo abelii GN=STK38 PE=1 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.159e-29
Identity = 56/125 (44.80%), Postives = 85/125 (68.00%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            SN TK++V   K  +EN Y   +   EER+ R+  LEK + +  + +EE+        +KETE++RL+R ++G +DFE L +IG+GAFGEVR+ ++  TGHVYAMK L+K++ML + QV H++AE
Sbjct:   13 SNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAE 137

HSP 2 Score: 38.891 bits (89), Expect = 1.159e-29
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERK 872
            E+LPGG MMTLLM++DTLTE +
Sbjct:  167 EFLPGGDMMTLLMKKDTLTEEE 188

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: STK38_MOUSE (Serine/threonine-protein kinase 38 OS=Mus musculus GN=Stk38 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.159e-29
Identity = 56/125 (44.80%), Postives = 85/125 (68.00%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            SN TK++V   K  +EN Y   +   EER+ R+  LEK + +  + +EE+        +KETE++RL+R ++G +DFE L +IG+GAFGEVR+ ++  TGHVYAMK L+K++ML + QV H++AE
Sbjct:   13 SNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAE 137

HSP 2 Score: 38.891 bits (89), Expect = 1.159e-29
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERK 872
            E+LPGG MMTLLM++DTLTE +
Sbjct:  167 EFLPGGDMMTLLMKKDTLTEEE 188

The following BLAST results are available for this feature:

BLAST of EY659590 vs. ExPASy Swiss-Prot

BLAST of EY659590 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34


Match Name	E-value	Identity	Description
NDRA_DICDI	2.910e-34	46.92	Probable serine/threonine-protein kinase ndrA OS=D...	[more]
SAX1_CAEEL	3.409e-31	43.20	Serine/threonine-protein kinase sax-1 OS=Caenorhab...	[more]
SAX1_CAEBR	5.751e-31	42.40	Serine/threonine-protein kinase sax-1 OS=Caenorhab...	[more]
CBK1_KLULA	1.618e-30	47.20	Serine/threonine-protein kinase CBK1 OS=Kluyveromy...	[more]
ST38L_HUMAN	3.096e-30	44.80	Serine/threonine-protein kinase 38-like OS=Homo sa...	[more]
CBK1_ASHGO	5.971e-30	45.31	Serine/threonine-protein kinase CBK1 OS=Ashbya gos...	[more]
CBK1_CANGA	7.739e-30	46.40	Serine/threonine-protein kinase CBK1 OS=Candida gl...	[more]
CBK1_YEAST	1.005e-29	47.54	Serine/threonine-protein kinase CBK1 OS=Saccharomy...	[more]
STK38_PONAB	1.159e-29	44.80	Serine/threonine-protein kinase 38 OS=Pongo abelii...	[more]
STK38_MOUSE	1.159e-29	44.80	Serine/threonine-protein kinase 38 OS=Mus musculus...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-011-H04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659590 ID=EY659590; Name=EY659590; organism=Citrus sinensis; type=EST; length=925bp
CTTTCATTTGTTGTGACCGAAAATCGCGGGTTTCTTTAATTTGTCTCAGG
ATAGCAGATCTTTGATACTCTGGGAATCCTTGATTCGAATGAACCTAAAT
GTTTCCGTTTGTGAAATGAGATTCGTGATTGATGAGAGTATATTTTTCAG
TAATTTCTCAAATAATTTGTACATTTAGGAGTGTGACCGTCGGGGAAAGT
CATATATAGATTTGAAAAAAATGGACTCTGCAAGATGTTGGTTTAACAAG
CTGAGGTCAAAAGACAAGGTAAAGCCTTCCAAGAAAAAGGAAACTGCAGG
CCATGGAAAGGAGGTGTCAAAAGCTCAAACAAGTGAAGAAGTTCCATCAA
ATGTTACCAAGCAGAAGGTTGCAGCTGCAAAACAGTATATTGAAAACCAT
TACAAGAAGCAGATGAAAAGCCTCGAGGAGAGGAAGGAGCGACGCAATAT
TTTGGAAAAGAAGTTAGCTGATGCAGAGGTTTCTGAGGAAGAGCAAAATA
ATTTACTAAAGTATTTAGAGAAGAAGGAAACGGAATACATGCGCCTTCAG
AGGCATAAAATGGGTGCTGATGATTTTGAGCCATTGACCATGATAGGGAA
GGGTGCATTTGGAGAGGTTAGAGTATGTAGGGAGAATGCAACCGGCCATG
TATATGCTATGAAAAAGCTTAAGAAATCAGAGATGCTTCGTAGAGGACAG
GTTGAACATGTGAAAGCTGAAGAAATCTACTTGCTGAAGNTGATAGNCAT
TGTATTGGTAAGCTATATTGTTCCTTTCCAGATGACGAGTATCTATATCT
GATTATGAATATCTACCTGGTGGNAGTATGATGACTTTACTGATGCGTAG
GGACACATTGACTGAAAGGAAGCCAGNTTTATTGTTGGGAAACTGTNCTA
GCTATTGATCTTNCCTAAACATAAT