DR405978

Overview
NameDR405978
Unique NameDR405978
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length711
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10693 contig Csv1_Contig10693:103..828. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN7_ARATH (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana GN=MAN7 PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 7.809e-68
Identity = 111/169 (65.68%), Postives = 132/169 (78.11%), Query Frame = 1
Query:  103 MKLLHLAFTIFLAFFIHRQSFNV-LHVEAGDGFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            MKLL  A   FLA  I    + +        GF+RT G  F LNG  YYANGFNAYWLMYV SDPSQRSK+STAF +A+ HGLTVARTWAFSDGGYR LQ SPGSYNE MF+GLDF +AEAR++GIK+ILS ANNY+SFGG+KQYV+WARS+G+ ++S+DDFF + +VK
Sbjct:    1 MKLL--ALFPFLAIVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSRGRPVSSEDDFFTDSLVK 167          

HSP 2 Score: 58.5362 bits (140), Expect = 7.809e-68
Identity = 23/26 (88.46%), Postives = 25/26 (96.15%), Query Frame = 2
Query:  632 YNTFTGIHYKDDPTIMAWELMNEPRC 709
            +NTFT +HYKDDPTIMAWELMNEPRC
Sbjct:  181 FNTFTKVHYKDDPTIMAWELMNEPRC 206          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN1_ORYSJ (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica GN=MAN1 PE=2 SV=2)

HSP 1 Score: 216.468 bits (550), Expect = 7.760e-65
Identity = 104/142 (73.24%), Postives = 119/142 (83.80%), Query Frame = 1
Query:  187 GDGFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGY-RPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVKG 609
            G GF+R  G+ F+L+GN YYANGFNAYWLM + +DPSQR KVS A  EAA HGLTVARTWAFSDGG    LQ SPG+YNE  FKGLDFV++EARKYGIK+ILSL +NYDSFGG+KQYVNWAR+QGQ + SDD+FF NPVVKG
Sbjct:   34 GGGFVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRGKVSAALGEAAGHGLTVARTWAFSDGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKVILSLVDNYDSFGGRKQYVNWARAQGQGIGSDDEFFTNPVVKG 175          

HSP 2 Score: 51.9878 bits (123), Expect = 7.760e-65
Identity = 20/25 (80.00%), Postives = 23/25 (92.00%), Query Frame = 2
Query:  635 NTFTGIHYKDDPTIMAWELMNEPRC 709
            NT TG+ Y+DDPTI+AWELMNEPRC
Sbjct:  189 NTITGVAYRDDPTILAWELMNEPRC 213          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN4_SOLLC (Mannan endo-1,4-beta-mannosidase 4 OS=Solanum lycopersicum GN=MAN4 PE=1 SV=2)

HSP 1 Score: 201.445 bits (511), Expect = 5.853e-59
Identity = 93/142 (65.49%), Postives = 112/142 (78.87%), Query Frame = 1
Query:  184 AGDGFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVKG 609
            + + F+ T G+HF LNG   Y NGFNAYWLMY+  DPS R KV+  F +A+ + + VARTWAFS GG RPLQ +PG YNEQMF+GLDFVI+EA+KYGI LI+SL NN+D+FGGKKQYV WA  +GQ LTSDDDFF NP+VKG
Sbjct:   28 SNNNFVYTDGTHFALNGKSLYINGFNAYWLMYIAYDPSTRIKVTNTFQQASKYKMNVARTWAFSHGGSRPLQSAPGVYNEQMFQGLDFVISEAKKYGIHLIMSLVNNWDAFGGKKQYVEWAVQRGQKLTSDDDFFTNPMVKG 169          

HSP 2 Score: 47.3654 bits (111), Expect = 5.853e-59
Identity = 18/25 (72.00%), Postives = 22/25 (88.00%), Query Frame = 2
Query:  635 NTFTGIHYKDDPTIMAWELMNEPRC 709
            NT T + YKDDPTI++WEL+NEPRC
Sbjct:  183 NTITKVAYKDDPTILSWELINEPRC 207          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN4_ARATH (Putative mannan endo-1,4-beta-mannosidase 4 OS=Arabidopsis thaliana GN=MAN4 PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 3.128e-57
Identity = 93/164 (56.71%), Postives = 120/164 (73.17%), Query Frame = 1
Query:  118 LAFTIFLAFFIHRQSFNVLHVEAGDGFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGG-YRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            L F + LA  I  QS+  +     DGF+   G  F+LNG  +YANGFNAYWL Y  +DP+ R K++  F  A S GLT+ARTW F DG  YR LQ +PGSY+EQ F+GLDFVIAEA++ GIKLI+ L NN+D +GGKKQYV+WARS+G+ ++S+DDF+RNPV+K
Sbjct:    4 LCFIVLLAIVI-AQSYVGVEAAPSDGFVSRNGVQFILNGKPFYANGFNAYWLAYEATDPTTRFKITNVFQNATSLGLTIARTWGFRDGAIYRALQTAPGSYDEQTFQGLDFVIAEAKRIGIKLIILLVNNWDDYGGKKQYVDWARSKGEVVSSNDDFYRNPVIK 166          

HSP 2 Score: 48.9062 bits (115), Expect = 3.128e-57
Identity = 19/25 (76.00%), Postives = 22/25 (88.00%), Query Frame = 2
Query:  635 NTFTGIHYKDDPTIMAWELMNEPRC 709
            NTFT + YKD+P IMAW+LMNEPRC
Sbjct:  181 NTFTKVAYKDEPAIMAWQLMNEPRC 205          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN9_ORYSJ (Putative mannan endo-1,4-beta-mannosidase 9 OS=Oryza sativa subsp. japonica GN=MAN9 PE=2 SV=2)

HSP 1 Score: 189.889 bits (481), Expect = 7.541e-56
Identity = 91/141 (64.54%), Postives = 104/141 (73.76%), Query Frame = 1
Query:  184 AGDGFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            A   F R  G+ F + G  +Y+NGFNAYWLMY+ SDP  RSK +    +AAS   T+ RTWAFSDGGYRPLQ+SPG YNE MF GLDFVIAEA+K G+ LILSL NN+D FGGKKQYV WAR QG  L SDDDFFR+ V K
Sbjct:   35 ANGQFARASGTRFTVGGRPFYSNGFNAYWLMYMASDPGDRSKAAGVLQQAASLRATLVRTWAFSDGGYRPLQKSPGVYNEDMFMGLDFVIAEAKKRGLYLILSLVNNWDGFGGKKQYVQWARDQGHNLGSDDDFFRSDVTK 175          

HSP 2 Score: 48.521 bits (114), Expect = 7.541e-56
Identity = 19/25 (76.00%), Postives = 21/25 (84.00%), Query Frame = 2
Query:  635 NTFTGIHYKDDPTIMAWELMNEPRC 709
            N  TG+ YKDDPTI AWEL+NEPRC
Sbjct:  188 NKITGVAYKDDPTIFAWELINEPRC 212          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN4_ORYSJ (Mannan endo-1,4-beta-mannosidase 4 OS=Oryza sativa subsp. japonica GN=MAN4 PE=2 SV=2)

HSP 1 Score: 181.03 bits (458), Expect = 2.366e-54
Identity = 85/144 (59.03%), Postives = 110/144 (76.39%), Query Frame = 1
Query:  178 VEAGDGFIRT*GSHFLLN-GNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            V  GDG +   G+ F+++ G   + +GFNAYWLM + +DP+ R  V+TAF +A++HGL +ARTWAFSDGG +PLQ SPG YNE MF+GLDFVIAEAR++GI L+L L NN+D+FGGK+QYV WA   G  LTSDDDFF + +VK
Sbjct:   17 VAPGDGMVAVDGTQFVVDCGRTIFFSGFNAYWLMMMAADPALRGAVATAFQQASAHGLNLARTWAFSDGGDQPLQSSPGVYNETMFQGLDFVIAEARRHGIYLLLCLTNNFDNFGGKRQYVRWAGDAGHNLTSDDDFFTSTIVK 160          

HSP 2 Score: 52.373 bits (124), Expect = 2.366e-54
Identity = 21/25 (84.00%), Postives = 22/25 (88.00%), Query Frame = 2
Query:  635 NTFTGIHYKDDPTIMAWELMNEPRC 709
            NT TG+ YKDDPTI AWELMNEPRC
Sbjct:  175 NTLTGVAYKDDPTIFAWELMNEPRC 199          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN3_ARATH (Mannan endo-1,4-beta-mannosidase 3 OS=Arabidopsis thaliana GN=MAN3 PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 4.025e-54
Identity = 84/164 (51.22%), Postives = 115/164 (70.12%), Query Frame = 1
Query:  118 LAFTIFLAFFIHRQSFNV-LHVEAGDGFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            L F + LA  I + S ++ +   + DGF+   G  F+LNG  +YANGFNAYWL Y  +D + R K++  F  A  H LT+ RTW F DGGYR LQ +PG Y+E+ F+GLDF IAEA++ GIK+I++  NNY  FGG+KQYV+WA++ GQ ++SDDDF+ NP+VK
Sbjct:    4 LCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATDSTTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRLGIKMIITFVNNYSDFGGRKQYVDWAKNTGQNVSSDDDFYTNPLVK 167          

HSP 2 Score: 49.6766 bits (117), Expect = 4.025e-54
Identity = 19/25 (76.00%), Postives = 22/25 (88.00%), Query Frame = 2
Query:  635 NTFTGIHYKDDPTIMAWELMNEPRC 709
            NTFT + YKD+PTIM WELMNEP+C
Sbjct:  182 NTFTKVEYKDEPTIMGWELMNEPQC 206          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN5_SOLLC (Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum GN=MAN5 PE=2 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 8.916e-54
Identity = 93/170 (54.71%), Postives = 119/170 (70.00%), Query Frame = 1
Query:  106 KLLHLAFTIFLAFFIHRQSFNVLHVE--AGD-GFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            +L+   F +FL          VL  E  A D GF+R  G+HF LNG+ +  NGFN+YWLM+V ++PS+R KVS    EA+S GL+V RTWAFSDGG R LQ SPG Y+E++F+GLDFVI+EA+KYGI+LILS  NNY+ FGGK QYV WAR+ G  +  DDDF+ N + K
Sbjct:    6 RLISCIFVLFLLSLAFACEARVLLDENNANDQGFVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNAGAQINGDDDFYTNYITK 175          

HSP 2 Score: 48.1358 bits (113), Expect = 8.916e-54
Identity = 20/25 (80.00%), Postives = 22/25 (88.00%), Query Frame = 2
Query:  632 YNTFTGIHYKDDPTIMAWELMNEPR 706
            +NT TG+ YKDD TIMAWELMNEPR
Sbjct:  189 FNTITGMTYKDDSTIMAWELMNEPR 213          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN2_SOLLC (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum GN=MAN2 PE=2 SV=2)

HSP 1 Score: 183.341 bits (464), Expect = 8.916e-54
Identity = 93/170 (54.71%), Postives = 119/170 (70.00%), Query Frame = 1
Query:  106 KLLHLAFTIFLAFFIHRQSFNVLHVE--AGD-GFIRT*GSHFLLNGNLYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            +L+   F +FL          VL  E  A D GF+R  G+HF LNG+ +  NGFN+YWLM+V ++PS+R KVS    EA+S GL+V RTWAFSDGG R LQ SPG Y+E++F+GLDFVI+EA+KYGI+LILS  NNY+ FGGK QYV WAR+ G  +  DDDF+ N + K
Sbjct:    6 RLISCIFVLFLLSLAFACEARVLLDENNANDQGFVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNAGAQINGDDDFYTNYITK 175          

HSP 2 Score: 48.1358 bits (113), Expect = 8.916e-54
Identity = 20/25 (80.00%), Postives = 22/25 (88.00%), Query Frame = 2
Query:  632 YNTFTGIHYKDDPTIMAWELMNEPR 706
            +NT TG+ YKDD TIMAWELMNEPR
Sbjct:  189 FNTITGMTYKDDSTIMAWELMNEPR 213          
BLAST of DR405978 vs. ExPASy Swiss-Prot
Match: MAN3_ORYSJ (Mannan endo-1,4-beta-mannosidase 3 OS=Oryza sativa subsp. japonica GN=MAN3 PE=2 SV=2)

HSP 1 Score: 172.17 bits (435), Expect = 1.371e-51
Identity = 84/149 (56.38%), Postives = 106/149 (71.14%), Query Frame = 1
Query:  163 FNVLHVEAGDGFIRT*GSHFLLNGN-LYYANGFNAYWLMYVDSDPSQRSKVSTAFHEAASHGLTVARTWAFSDGGYRPLQRSPGSYNEQMFKGLDFVIAEARKYGIKLILSLANNYDSFGGKKQYVNWARSQGQFLTSDDDFFRNPVVK 606
            F      AG G +   G+ F++ G    Y +GFNAYWLM + SDP++R+ V  AF +A+S GL +ARTWAFSDGG +PLQ SPG Y+E MF+GLDFVIAEAR++GI L+L L NN+D FGGK+QYV WA   G  LT+ DDFF + VVK
Sbjct:   18 FGAAAAAAGGGMVGVDGTQFVVEGGRTIYFSGFNAYWLMMMASDPARRAAVVAAFAQASSRGLNLARTWAFSDGGDQPLQSSPGVYDEAMFQGLDFVIAEARRHGIYLLLCLTNNFDDFGGKRQYVRWAADAGHNLTAGDDFFTSSVVK 166          

HSP 2 Score: 51.9878 bits (123), Expect = 1.371e-51
Identity = 21/25 (84.00%), Postives = 22/25 (88.00%), Query Frame = 2
Query:  635 NTFTGIHYKDDPTIMAWELMNEPRC 709
            NT TG+ YKDDPTI AWELMNEPRC
Sbjct:  181 NTVTGVAYKDDPTIFAWELMNEPRC 205          
The following BLAST results are available for this feature:
BLAST of DR405978 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 22
Match NameE-valueIdentityDescription
MAN7_ARATH7.809e-6865.68Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis ... [more]
MAN1_ORYSJ7.760e-6573.24Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa... [more]
MAN4_SOLLC5.853e-5965.49Mannan endo-1,4-beta-mannosidase 4 OS=Solanum lyco... [more]
MAN4_ARATH3.128e-5756.71Putative mannan endo-1,4-beta-mannosidase 4 OS=Ara... [more]
MAN9_ORYSJ7.541e-5664.54Putative mannan endo-1,4-beta-mannosidase 9 OS=Ory... [more]
MAN4_ORYSJ2.366e-5459.03Mannan endo-1,4-beta-mannosidase 4 OS=Oryza sativa... [more]
MAN3_ARATH4.025e-5451.22Mannan endo-1,4-beta-mannosidase 3 OS=Arabidopsis ... [more]
MAN5_SOLLC8.916e-5454.71Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lyco... [more]
MAN2_SOLLC8.916e-5454.71Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lyco... [more]
MAN3_ORYSJ1.371e-5156.38Mannan endo-1,4-beta-mannosidase 3 OS=Oryza sativa... [more]

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Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248F20 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248F20 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR405978 ID=DR405978|Name=DR405978|organism=Citrus sinensis|type=EST|length=711bp
GGGGGCAGTTCTAAGAGGAAAAACTTCACGAGTTCACTAATAAAAATATA
GCCTCATCTCCACTGCTCCTTATCCACTCAGACACAGAGAGAGTGAAAGA
GGATGAAGCTCTTGCATTTAGCTTTCACAATCTTCTTAGCCTTTTTCATC
CACCGGCAATCTTTCAACGTCCTACATGTTGAAGCAGGAGATGGATTTAT
AAGAACATGAGGATCGCATTTTCTGTTGAATGGTAATCTTTACTATGCAA
ATGGATTTAATGCATATTGGTTGATGTATGTAGATTCTGACCCATCTCAG
AGATCCAAAGTATCAACTGCTTTTCATGAAGCTGCAAGCCATGGCCTCAC
TGTTGCCAGAACTTGGGCTTTCAGTGATGGTGGTTACAGACCCTTACAGC
GTTCCCCTGGCTCCTACAATGAGCAAATGTTTAAGGGATTGGATTTTGTG
ATAGCTGAGGCTAGAAAGTATGGGATCAAGCTCATATTGAGCTTGGCCAA
CAACTATGATAGTTTTGGAGGGAAGAAGCAGTATGTGAACTGGGCTAGAA
GTCAAGGCCAATTCCTCACATCTGATGATGATTTCTTCAGAAACCCTGTT
GTTAAGGGTTACTACAAGAACCTTATCAAGATATAATACCTTTACTGGAA
TTCACTACAAAGATGACCCGACAATTATGGCATGGGAGCTTATGAATGAG
CCGAGATGCAC
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