DN617456

Overview
NameDN617456
Unique NameDN617456
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length559
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13335 contig Csv1_Contig13335:866..1423. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 279.256 bits (713), Expect = 8.950e-75
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL
Sbjct:  452 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 1.292e-73
Identity = 130/133 (97.74%), Postives = 132/133 (99.25%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR DPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITD+IHPAGWHEWDGNFALNTLFYGEHQN+GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL
Sbjct:  452 AQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 5.442e-64
Identity = 115/133 (86.47%), Postives = 122/133 (91.73%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR DPNQNTGIVIQK RIGATSDL+PVQ SFPTYLGRPWKEYSRTVIMQSSITDVI PAGWHEW+GNFAL+TLFYGE+ N GAGA TSGRVKWKG +VITS+TEAQA+TPG FIAG SWL STGFPFSLGL
Sbjct:  451 AQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME_DAUCA (Pectinesterase OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 1.341e-62
Identity = 112/133 (84.21%), Postives = 122/133 (91.73%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR DPNQNTGIVIQKSRIGATSDL+PVQ SFPTYLGRPWKEYSRTV+MQSSIT+VI+PAGW  WDGNFAL+TL+YGE+QN GAGA TSGRV WKGF+VITS+TEAQ FTPGSFIAG SWL +T FPFSLGL
Sbjct:  187 AQGRTDPNQNTGIVIQKSRIGATSDLQPVQSSFPTYLGRPWKEYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAATSGRVTWKGFKVITSSTEAQGFTPGSFIAGGSWLKATTFPFSLGL 319
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 216.468 bits (550), Expect = 7.125e-56
Identity = 101/133 (75.94%), Postives = 112/133 (84.21%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR DPNQNTGIVIQK RIGATSDL+ V+GSFPTYLGRPWKEYS+TVIMQS+I+DVI P GW EW G FALNTL Y E+ N GAGAGT+ RVKW+GF+VIT+A EAQ +T G FI G  WL STGFPFSLGL
Sbjct:  460 AQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 211.46 bits (537), Expect = 2.292e-54
Identity = 99/133 (74.44%), Postives = 109/133 (81.95%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR+DPNQNTGIVIQ  RIG TSDL  V+G+FPTYLGRPWKEYSRTVIMQS I+DVI P GWHEW G+FAL+TL Y E+ N G GAGT+ RVKWKG++VITS TEAQ FT G FI G  WL STGFPFSL L
Sbjct:  455 AQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 182.956 bits (463), Expect = 8.729e-46
Identity = 86/133 (64.66%), Postives = 103/133 (77.44%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR+DPNQNTGI+I  SR+ A SDL+PV GS  TYLGRPW++YSRTV M++S+  +I P GW EWDGNFAL TLFY E QN G GA TSGRV W GFRV+ SA+EA  FT G+F+AG SW+ S+  PF+ GL
Sbjct:  380 AQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME1_FICAW (Pectinesterase/pectinesterase inhibitor OS=Ficus awkeotsang PE=1 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 7.390e-45
Identity = 84/133 (63.16%), Postives = 101/133 (75.94%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR DPNQNTG  IQ   I A++DL+PV+ +F +YLGRPWKEYSRTV+M+S I+DVI PAGW EWD +FAL TLFYGE++N G G+GTS RVKW G+ VITS   A+ FT    I G SWLGSTG  ++ GL
Sbjct:  411 AQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAELIQGGSWLGSTGVDYTAGL 543
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 1.067e-43
Identity = 77/133 (57.89%), Postives = 102/133 (76.69%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR DPNQ+TGI+I   R+ A SDLKPVQ S  T+LGRPWK+YSRTV +++ +  +I+PAGW EW G+FALNTL+Y E+ N G G+ T+ RVKW+G+ V+TS ++   FT G+FIAG+SWL +T  PF+ GL
Sbjct:  378 AQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510
[Close]
BLAST of DN617456 vs. ExPASy Swiss-Prot
Match: PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 168.703 bits (426), Expect = 1.704e-41
Identity = 82/133 (61.65%), Postives = 94/133 (70.68%), Query Frame = -3 
Query:  159 AQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 557
            AQGR DPNQ TG  IQ   I A+SDL+PV   FPTYLGRPWKEYSRTV+M+S +  +I+PAGW EWDG+FAL TL+YGE  N G GAGTS RVKW G+ VIT   +A  FT    I G SWL STG  +  GL
Sbjct:  412 AQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRSTGVAYVDGL 544
The following BLAST results are available for this feature:
BLAST of DN617456 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 70
Match NameE-valueIdentityDescription
PME3_CITSI8.950e-75100.00Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME1_CITSI1.292e-7397.74Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE... [more]
PMEU1_SOLLC5.442e-6486.47Pectinesterase/pectinesterase inhibitor U1 OS=Sola... [more]
PME_DAUCA1.341e-6284.21Pectinesterase OS=Daucus carota PE=1 SV=1[more]
PME3_ARATH7.125e-5675.94Pectinesterase/pectinesterase inhibitor 3 OS=Arabi... [more]
PME2_ARATH2.292e-5474.44Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 P... [more]
PME17_ARATH8.729e-4664.66Probable pectinesterase/pectinesterase inhibitor 1... [more]
PME1_FICAW7.390e-4563.16Pectinesterase/pectinesterase inhibitor OS=Ficus a... [more]
PME2_CITSI1.067e-4357.89Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE... [more]
PME1_SOLLC1.704e-4161.65Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_01D18_f Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0001D18, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN617456 ID=DN617456; Name=DN617456; organism=Citrus sinensis; type=EST; length=559bp
TCCCATGCTATTTTATTTCTCTGTTAATCACACAATAGTATTTGTGAAAT
CGAAGCCACGAATAAGACACGCTCAATCAAATATTACAAATTGAAAGACC
AGAGACCTATGGATCCACTAATACAAAACATATTAATTAAAACTCCTAGT
GAATATTACAAACCAAGGGAGAATGGGAAACCAGTGGAGCCCAGCCAGCT
ACTACCAGCAATGAAGCTTCCAGGAGTAAAAGCTTGAGCCTCGGTAGCAC
TTGTAATAACCCTAAATCCCTTCCATTTCACTCTCCCTGAAGTTCCGGCA
CCGGCTCCGGCGTTCTGATGCTCTCCGTAAAACAATGTGTTCAACGCGAA
GTTACCATCCCACTCGTGCCACCCGGCAGGGTGGATCACGTCAGTAATCG
ATGACTGCATGATCACCGTCCTCGAGTACTCCTTCCAGGGCCTGCCGAGG
TACGTCGGGAAACTACCCTGAACCGGTTTTAAATCGGAGGTGGCACCAAT
CCTAGATTTTTGAATGACAATGCCGGTGTTTTGGTTAGGGTCAGCCCTGC
CTTGGGCTG
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