Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map

Publication Overview
TitleEvaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map
AuthorsSankar A, Moore G
TypeJournal Article
Journal NameTheoretical and applied genetics
Volume102
Issue2/3
Year2001
Page(s)206-214
CitationSankar A, Moore G. Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map. Theoretical and applied genetics. 2001 Feb; 102(2/3):206-214.

Abstract

Inter-simple sequence repeat (ISSR) analysis was evaluated for its usefulness in generating markers to extend the genetic linkage map of Citrus using a back-cross population previously mapped with restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and isozyme markers. ISSR markers were obtained through the simple technique of PCR followed by analysis on agarose gels, using simple sequence repeat (SSR) primers. Optimization of reaction conditions was achieved for 50% of the SSR primers screened, and the primers amplified reproducible polymorphic bands in the parents and progeny of the back-cross population. Mendelian segregation of the polymorphic bands was demonstrated, with an insignificant number of skewed loci. Most of the SSR primers produced dominant loci; however co-dominance was observed with loci derived from three primers. A new genetic map was produced by combining the segregation data for the ISSR markers and data for the RFLP, RAPD and isozyme markers from the previous map and creating genetic linkages among all the markers using JoinMap 2.0 mapping software. The new map has an improved distribution of markers along the linkage groups with fewer gaps, and marker order showed partial or complete conservation in the linkage groups. The incorporation of ISSR markers into the genetic linkage map demonstrates that ISSR markers are suitable for genetic mapping in Citrus.
Featuremaps
This publication contains information about 1 maps:
Map Name
Citrus-Pomeroy/USDA17-40-BC1-1992-1
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
ThongDeeThongDeebreeding_research_material
USDA17-40USDA17-40cultivar
PomeroyPomeroycultivar
Citrus-Pomeroy/USDA17-40-BC1Citrus-Pomeroy/USDA17-40-BC1population
Features
This publication contains information about 138 features:
Feature NameUniquenameType
IdhIdhgenetic_marker
IS1IS1genetic_marker
IS5IS5genetic_marker
IS7IS7genetic_marker
IS8IS8genetic_marker
OPA01OPA01genetic_marker
OPA04OPA04genetic_marker
OPA05OPA05genetic_marker
OPA09OPA09genetic_marker
OPA10OPA10genetic_marker
OPA11OPA11genetic_marker
OPA12OPA12genetic_marker
OPA14OPA14genetic_marker
OPA15OPA15genetic_marker
OPA18OPA18genetic_marker
OPA19OPA19genetic_marker
OPE01OPE01genetic_marker
OPE03OPE03genetic_marker
OPE04OPE04genetic_marker
OPE05OPE05genetic_marker
OPE07OPE07genetic_marker
OPE08OPE08genetic_marker
OPE12OPE12genetic_marker
OPE14OPE14genetic_marker
OPE15OPE15genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Journal AbbreviationTheor. appl. genet.
Language Abbreng
Publication Date2001 Feb
Publication TypeJournal Article
URLhttp://link.springer.com/article/10.1007/s001220051637
KeywordsCitrus maxima, Poncirus trifoliata, interspecific hybridization, backcrossing, genetic markers, chromosome mapping, microsatellite repeats, linkage groups, polymerase chain reaction, repetitive sequences, segregation
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3502914