Genetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliata (L.) Raf. and mapping of citrus tristeza virus resistance gene

Publication Overview
TitleGenetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliata (L.) Raf. and mapping of citrus tristeza virus resistance gene
AuthorsCristofani M, Machado M, Grattapaglia D
TypeJournal Article
Journal NameEuphytica
Volume109
Issue1
Year1999
Page(s)25-32
CitationCristofani M, Machado M, Grattapaglia D. Genetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliata (L.) Raf. and mapping of citrus tristeza virus resistance gene. Euphytica : Netherlands journal of plant breeding. 1999; 109(1):25-32.

Abstract

A linkage map for Citrus sunki Hort. ex. Tan. and Poncirus trifoliate (L.) Raf cv. Rubidoux has been developed exploiting the pseudo-testcross mapping strategy and RAPD markers. From controlled crosses 314 F1 zygotic progeny individuals were obtained. Two separate data sets were generated, one for each parent. Preliminary linkage analysis was carried out with a phase-unknown backcross model at a LOD > 4.0 and max. Theta = 0.30. A total of 203 arbitrary 10-mer primers and 5 arbitrary 8-mer primers were screened for segregating RAPD markers. Pseudo-testcrosss mating configurations were displayed by 78 primers where the marker was present in one parent, absent in the other and segregating in the progeny. These 78 primers were run on 80 individuals of the progeny amplifying a total of 169 RAPD markers. Linkage analysis revealed that 125 of these RAPD loci fell into 18 linkage groups (10 groups for ‘Sunki’ and 8 groups for ‘Rubidoux’) while 44 loci remained unassigned to any linkage group. The total length of the maps was 732.32 cM for ‘Sunki’ and 866.88 cM for ‘Rubidoux’ with the distance between markers ranging from 0 to 43.8 cM. Bulked segregant analysis (BSA) was used to identify potential resistance-associated RAPD fragment markers. Two RAPD fragments were found flanking the CTV gene (OPAV12_470 - 17.8 cM and OPN15_1630 - 23.1 cM).
Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-Sunki/Rubidoux-Sunki-F1-1999
Citrus-Sunki/Rubidoux-Rubidoux-F1-1999
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
Citrus sunkiCitrus sunkicultivar
RubidouxRubidouxcultivar
Citrus-Sunki/Rubidoux-Sunki-F1Citrus-Sunki/Rubidoux-Sunki-F1population
Citrus-Sunki/Rubidoux-Rubidoux-F1Citrus-Sunki/Rubidoux-Rubidoux-F1population
Properties
Additional details for this publication include:
Property NameValue
Language Abbreng
Publication Date1999
Publication TypeJournal Article
KeywordsPoncirus trifoliata, Citrus tristeza virus, disease resistance, genetic resistance, genes, chromosome mapping, genetic markers, progeny, linkage (genetics), random amplified polymorphic DNA technique, segregation, gene location, citrus sunki
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3373967