CX308700

Overview
NameCX308700
Unique NameCX308700
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length307
Libraries
Library NameType
AbsLeaSub1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CX308700_ssr257 microsatellite CX308700_ssr257:257..268. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig9567 contig Ccv1_Contig9567:7..313. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX308700 vs. ExPASy Swiss-Prot
Match: RPB4_MOUSE (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus GN=Polr2d PE=2 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 1.360e-12
Identity = 39/100 (39.00%), Postives = 64/100 (64.00%), Query Frame = -2 
Query:    4 GKTKPRAFRMSGEEEENAAELKIGDEFLKAKCLMNCEVAIILDHKYEQLQQTSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFEL 303
            G + PRA    G+ EE+A++L    EF  A+ L+N EV ++L+H+ +Q +   D+   ++S+VF K+L Y  RFSR+KN + +  VR +L + +L +FEL
Sbjct:    4 GGSDPRA----GDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFEL 97
[Close]
BLAST of CX308700 vs. ExPASy Swiss-Prot
Match: RPB4_HUMAN (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens GN=POLR2D PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.360e-12
Identity = 39/100 (39.00%), Postives = 64/100 (64.00%), Query Frame = -2 
Query:    4 GKTKPRAFRMSGEEEENAAELKIGDEFLKAKCLMNCEVAIILDHKYEQLQQTSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFEL 303
            G + PRA    G+ EE+A++L    EF  A+ L+N EV ++L+H+ +Q +   D+   ++S+VF K+L Y  RFSR+KN + +  VR +L + +L +FEL
Sbjct:    4 GGSDPRA----GDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFEL 97
[Close]
BLAST of CX308700 vs. ExPASy Swiss-Prot
Match: RPB4_DICDI (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum GN=polr2d PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.465e-11
Identity = 38/111 (34.23%), Postives = 58/111 (52.25%), Query Frame = -2 
Query:    4 TKPRAFRMSGEEEENAAELKIGDEFLKAKCLMNCEVAIILDHKYEQLQQTSDD-------------PMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFEL 297
            T P   R   +EEE+   LK   +   AK L+N EVAI+L+H+    +    +              +N + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+
Sbjct:    2 TDPLIKRSGIQEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSINDIIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEI 112
The following BLAST results are available for this feature:
BLAST of CX308700 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 3
Match NameE-valueIdentityDescription
RPB4_MOUSE1.360e-1239.00DNA-directed RNA polymerase II subunit RPB4 OS=Mus... [more]
RPB4_HUMAN1.360e-1239.00DNA-directed RNA polymerase II subunit RPB4 OS=Hom... [more]
RPB4_DICDI7.465e-1134.23DNA-directed RNA polymerase II subunit rpb4 OS=Dic... [more]
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Properties
Property NameValue
Genbank descriptionC21002H02Rv AbsLeaSub1 Citrus clementina cDNA clone C21002H02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX308700 ID=CX308700; Name=CX308700; organism=Citrus clementina; type=EST; length=307bp
ACACAGCTCGAATTCAGCCAACTGGTATCTACTAAGGATTTCTCGCACTT
GTCTAACAGCATCAGGATTCTTGTAGCGACTAAAGCGCTTCACATACTGC
AGTGATTTCTCAAACACTTGGGAAACTTGATTCATGGGATCATCAGAAGT
CTGCTGAAGCTGCTCATACTTGTGATCAAGAATTATTGCTACTTCACAAT
TCATCAAACACTTGGCCTTCAAAAACTCGTCTCCAATTTTAAGCTCGGCC
GCGTTCTCTTCTTCTTCTCCAGACATTCTGAAAGCTCTTGGCTTTGTTTT
TCCCCCG
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