Identification of closely related citrus cultivars with inter-simple sequence repeat markers

Publication Overview
TitleIdentification of closely related citrus cultivars with inter-simple sequence repeat markers
AuthorsFang D, Roose M
TypeJournal Article
Journal NameTheoretical and applied genetics
CitationFang D, Roose M. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theoretical and applied genetics. 1997 Aug; 95(3):408-417.


Inter-simple sequence repeat (ISSR) markers generated by 22 primers were tested for their ability to distinguish among samples from 94 trees of 68 citrus cultivars. Within each of the six cultivar groups studied most of these cultivars are so closely related that they are difficult to distinguish by other molecular-marker techniques. ISSR markers involve PCR amplification of DNA using a single primer composed of a microsatellite sequence anchored at the 3' or 5' end by 2-4 arbitrary, often degenerate, nucleotides. The amplification products were separated on non-denaturing polyacrylamide gels and detected by silver staining. ISSR banding profiles were very repeatable on duplicate samples. Different citrus species had very different fingerprint patterns. Within Citrus sinensis (L.) Osbeck and C. paradisi Macf., in which all cultivars have originated by the selection of mutants, ISSR markers distinguished 14 of 33 sweet orange and 1 of 7 grapefruit cultivars. Five of six lemon cultivars were discriminated by ISSR markers. Many differences were found among mandarin cultivars; however, all five satsuma cultivars analyzed had identical ISSR fingerprints. Four of five citrange cultivars were distinguishable, but 'Troyer' and 'Carrizo' had identical ISSR fingerprints. 'Kuharske Carrizo' citrange, which has better citrus nematode resistance than other 'Carrizo' citrange accessions, had unique ISSR fingerprints. Three ISSR markers that differentiated certain sweet orange cultivars were hybridized to Southern blots of sweet orange DNA digested with different restriction endonucleases. The sweet orange cultivars tested could be distinguished by these ISSR-derived RFLP markers. Moreover, one ISSR marker unique to 'Ruby' blood orange was observed in its progeny trees.
This publication contains information about 65 stocks:
Stock NameUniquenameType
Atwood (old line)Atwood (old line)cultivar
Campbell nucellarCampbell nucellarcultivar
Cara Cara pinkCara Cara pinkcultivar
Carrizo (seedling)Carrizo (seedling)breeding_research_material
Clementine AlgerianClementine Algeriancultivar
Clementine MonrealClementine Monrealcultivar
Corrigated ThomsonCorrigated Thomsoncultivar
Cutter nucellarCutter nucellarcultivar
Dobashi Beni satsumaDobashi Beni satsumacultivar
Dream NucellarDream Nucellarcultivar
Dry navelDry navelcultivar
Eddy (old line)Eddy (old line)cultivar
Fisher (old line)Fisher (old line)cultivar


Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date1997 Aug
Language Abbreng
Journal AbbreviationTheor. appl. genet.
KeywordsCitrus sinensis, Citrus paradisi, Citrus limon, Citrus reticulata, Citroncirus webberi, Poncirus trifoliata, genetic markers, cultivar identification, DNA fingerprinting, restriction fragment length polymorphism, repetitive sequences, species differences
Cross References
This publication is also available in the following databases:
AGL: USDA National Agricultural LibraryAGL:3274633