Developing fruit juice sac at 38 DAFB

Overview
Library NameDeveloping fruit juice sac at 38 DAFB
Unique NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK933165CK933165EST
CK933166CK933166EST
CK933167CK933167EST
CK933168CK933168EST
CK933169CK933169EST
CK933170CK933170EST
CK933171CK933171EST
CK933172CK933172EST
CK933173CK933173EST
CK933174CK933174EST
CK933175CK933175EST
CK933176CK933176EST
CK933177CK933177EST
CK933178CK933178EST
CK933179CK933179EST
CK933180CK933180EST
CK933181CK933181EST
CK933182CK933182EST
CK933183CK933183EST
CK933184CK933184EST
CK933185CK933185EST
CK933186CK933186EST
CK933187CK933187EST
CK933188CK933188EST
CK933189CK933189EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stage38 DAFB fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type