Developing fruit flavedo at 80 DAFB

Overview
Library NameDeveloping fruit flavedo at 80 DAFB
Unique NameDeveloping fruit flavedo at 80 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The flavedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit flavedo at 80 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CK936550CK936550EST
CK936551CK936551EST
CK936552CK936552EST
CK936553CK936553EST
CK936554CK936554EST
CK936555CK936555EST
CK936556CK936556EST
CK936557CK936557EST
CK936558CK936558EST
CK936559CK936559EST
CK936560CK936560EST
CK936561CK936561EST
CK936562CK936562EST
CK936563CK936563EST
CK936564CK936564EST
CK936565CK936565EST
CK936566CK936566EST
CK936567CK936567EST
CK936568CK936568EST
CK936569CK936569EST
CK936570CK936570EST
CK936571CK936571EST
CK936572CK936572EST
CK936573CK936573EST
CK936574CK936574EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit sample-collected July 29, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The flavedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type