Ruby Orange Developing Flower cDNA Library UCRCS04-UCR

Overview
Library NameRuby Orange Developing Flower cDNA Library UCRCS04-UCR
Unique NameRuby Orange Developing Flower cDNA Library UCRCS04-UCR
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January -March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Genomics Core Instrumentation Facility, University of California, Riverside (Choi, Kingan). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
SNP Chip Base
Array NameRuby Orange Developing Flower cDNA Library UCRCS04-UCR
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CV884229CV884229EST
CV884230CV884230EST
CV884231CV884231EST
CV884232CV884232EST
CV884233CV884233EST
CV884234CV884234EST
CV884235CV884235EST
CV884236CV884236EST
CV884237CV884237EST
CV884238CV884238EST
CV884239CV884239EST
CV884240CV884240EST
CV884241CV884241EST
CV884242CV884242EST
CV884243CV884243EST
CV884244CV884244EST
CV884245CV884245EST
CV884246CV884246EST
CV884247CV884247EST
CV884248CV884248EST
CV884249CV884249EST
CV884250CV884250EST
CV884251CV884251EST
CV884252CV884252EST
CV884253CV884253EST

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Properties
Property NameValue
Genbank library cultivarRuby
Genbank library dev stage20 year old trees
Genbank library noteVector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January -March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Genomics Core Instrumentation Facility, University of California, Riverside (Choi, Kingan). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Genbank library tissue typeFlower
Flowertissue type