Mature Albedo

Overview
Library NameMature Albedo
Unique NameMature Albedo
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Albedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameMature Albedo
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CB611076CB611076EST
CB611077CB611077EST
CB611078CB611078EST
CB611079CB611079EST
CB611080CB611080EST
CB611081CB611081EST
CB611082CB611082EST
CB611083CB611083EST
CB611084CB611084EST
CB611085CB611085EST
CB611086CB611086EST
CB611087CB611087EST
CB611088CB611088EST
CB611089CB611089EST
CB611090CB611090EST
CB611091CB611091EST
CB611092CB611092EST
CB611093CB611093EST
CB611094CB611094EST
CB611095CB611095EST
CB611096CB611096EST
CB611097CB611097EST
CB611098CB611098EST
CB611099CB611099EST
CB611100CB611100EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Albedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Pericarptissue type