Developing fruit albedo at 80 DAFB in pTriplEx2 vector

Overview
Library NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
Unique NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CK937573CK937573EST
CK937574CK937574EST
CK937575CK937575EST
CK937576CK937576EST
CK937577CK937577EST
CK937578CK937578EST
CK937579CK937579EST
CK937580CK937580EST
CK937581CK937581EST
CK937582CK937582EST
CK937583CK937583EST
CK937584CK937584EST
CK937585CK937585EST
CK937586CK937586EST
CK937587CK937587EST
CK937588CK937588EST
CK937589CK937589EST
CK937590CK937590EST
CK937591CK937591EST
CK937592CK937592EST
CK937593CK937593EST
CK937594CK937594EST
CK937595CK937595EST
CK937596CK937596EST
CK937597CK937597EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected July 29, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type