Washington Navel orange cold acclimated flavedo & albedo cDNA library

Overview
Library NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
Unique NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the field at University of California, Riverside Agricultural Operations since 1983. The scion was Washington Navel orange and the rootstock Carizzo Citrange. Tissue from mature fruit was harvested at mid-day in January 2002 during a cold spell, when pre-dawn temperatures were approximately -2 to 2 degree C. Approximately 2 cm median sections of the rind were excised in the field from several fruits, then wrapped in aluminum foil and frozen quickly in dry ice. Total RNA was extracted using a phenol extraction procedure described in J. Japanaese Soc. Hort. Sci. (1996) 64:809-814. Poly(A) RNA was purified, a cDNA library was made, and 1 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside (Fenton). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3530 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
SNP Chip Base
Array NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CB290585CB290585EST
CB290586CB290586EST
CB290587CB290587EST
CB290588CB290588EST
CB290589CB290589EST
CB290590CB290590EST
CB290591CB290591EST
CB290592CB290592EST
CB290593CB290593EST
CB290594CB290594EST
CB290595CB290595EST
CB290596CB290596EST
CB290597CB290597EST
CB290598CB290598EST
CB290599CB290599EST
CB290600CB290600EST
CB290601CB290601EST
CB290602CB290602EST
CB290603CB290603EST
CB290604CB290604EST
CB290605CB290605EST
CB290606CB290606EST
CB290607CB290607EST
CB290608CB290608EST
CB290611CB290611EST

Pages

Properties
Property NameValue
Pericarptissue type
Genbank library cultivarWashington navel
Genbank library dev stageMature fruit
Genbank library noteVector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the field at University of California, Riverside Agricultural Operations since 1983. The scion was Washington Navel orange and the rootstock Carizzo Citrange. Tissue from mature fruit was harvested at mid-day in January 2002 during a cold spell, when pre-dawn temperatures were approximately -2 to 2 degree C. Approximately 2 cm median sections of the rind were excised in the field from several fruits, then wrapped in aluminum foil and frozen quickly in dry ice. Total RNA was extracted using a phenol extraction procedure described in J. Japanaese Soc. Hort. Sci. (1996) 64:809-814. Poly(A) RNA was purified, a cDNA library was made, and 1 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside (Fenton). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3530 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Genbank library tissue typeRind containing flavedo and albedo