Developing fruit albedo at 80 DAFB in Vulnus5 vector

Overview
Library NameDeveloping fruit albedo at 80 DAFB in Vulnus5 vector
Unique NameDeveloping fruit albedo at 80 DAFB in Vulnus5 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: Vulnus5; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit albedo at 80 DAFB in Vulnus5 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
DN134838DN134838EST
DN134840DN134840EST
DN134841DN134841EST
DN134842DN134842EST
DN134843DN134843EST
DN134844DN134844EST
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DN134848DN134848EST
DN134849DN134849EST
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DN134851DN134851EST
DN134852DN134852EST
DN134853DN134853EST
DN134855DN134855EST
DN134856DN134856EST
DN134857DN134857EST
DN134858DN134858EST
DN134859DN134859EST
DN134860DN134860EST
DN134861DN134861EST
DN134862DN134862EST
DN134863DN134863EST
DN134864DN134864EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected July 29, 2003
Genbank library noteOrgan: Fruit; Vector: Vulnus5; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type