Developing fruit albedo at 165 DAFB

Overview
Library NameDeveloping fruit albedo at 165 DAFB
Unique NameDeveloping fruit albedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit albedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK938654CK938654EST
CK938655CK938655EST
CK938656CK938656EST
CK938657CK938657EST
CK938658CK938658EST
CK938659CK938659EST
CK938660CK938660EST
CK938661CK938661EST
CK938662CK938662EST
CK938663CK938663EST
CK938664CK938664EST
CK938665CK938665EST
CK938666CK938666EST
CK938667CK938667EST
CK938668CK938668EST
CK938669CK938669EST
CK938670CK938670EST
CK938672CK938672EST
CK938673CK938673EST
CK938674CK938674EST
CK938675CK938675EST
CK938676CK938676EST
CK938677CK938677EST
CK938678CK938678EST
CK938679CK938679EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected October 22, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type