Developing fruit 24 DAFB

Overview
Library NameDeveloping fruit 24 DAFB
Unique NameDeveloping fruit 24 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit 24 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CK935581CK935581EST
CK935582CK935582EST
CK935583CK935583EST
CK935584CK935584EST
CK935585CK935585EST
CK935586CK935586EST
CK935587CK935587EST
CK935588CK935588EST
CK935589CK935589EST
CK935590CK935590EST
CK935591CK935591EST
CK935592CK935592EST
CK935593CK935593EST
CK935594CK935594EST
CK935595CK935595EST
CK935596CK935596EST
CK935597CK935597EST
CK935598CK935598EST
CK935599CK935599EST
CK935600CK935600EST
CK935601CK935601EST
CK935602CK935602EST
CK935603CK935603EST
CK935604CK935604EST
CK935605CK935605EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 3 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type