DY306218

Overview
NameDY306218
Unique NameDY306218
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1276
Libraries
Library NameType
CitNFLcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY306218 vs. ExPASy Swiss-Prot
Match: LCB5_YEAST (Sphingoid long chain base kinase 5 OS=Saccharomyces cerevisiae GN=LCB5 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.647e-15
Identity = 44/105 (41.90%), Postives = 60/105 (57.14%), Query Frame = 2 
Query:  422 RPKRLYIFVNPFGGKKIASKIFLDDVKPLLEDANIQFTVQETTQQLHAKEIVKGLDLSKYDGIVCVSGDGILVEVVNGLLEREDWNDAI-KVPLGVVPAXTGNGM 733
            R K +++ +NPFGGK  A K+F+   KPLL  +     V  T    HA EI + +D+ KYD I C SGDGI  EV+NGL +R D   A   + +  +P  +GN M
Sbjct:  267 RNKSIFVIINPFGGKGKAKKLFMTKAKPLLLASRCSIEVVYTKYPGHAIEIAREMDIDKYDTIACASGDGIPHEVINGLYQRPDHVKAFNNIAITEIPCGSGNAM 371
[Close]
BLAST of DY306218 vs. ExPASy Swiss-Prot
Match: CERK1_HUMAN (Ceramide kinase OS=Homo sapiens GN=CERK PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.793e-15
Identity = 47/125 (37.60%), Postives = 71/125 (56.80%), Query Frame = 2 
Query:  380 LWCEKLRDFIDSF-GRPKRLYIFVNPFGGKKIASKIFLDDVKPLLEDANIQFTVQETTQQLHAKEIVKGLDLSKYDGIVCVSGDGILVEVVNGLLER--------EDWNDAIKVP----LGVVPA 715
            LW + LR+ ++    RPK L +F+NPFGGK    +I+   V PL   A+I   +  T     AKE +  +++ KYDGIVCV GDG+  EV++GL+ R        ++   A+ VP    +G++PA
Sbjct:  114 LWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIYERKVAPLFTLASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMFSEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPA 238
[Close]
BLAST of DY306218 vs. ExPASy Swiss-Prot
Match: CERK1_MOUSE (Ceramide kinase OS=Mus musculus GN=Cerk PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 5.299e-14
Identity = 48/135 (35.56%), Postives = 70/135 (51.85%), Query Frame = 2 
Query:  350 FQPLSEDSKRLWCEKLRDFIDSF-GRPKRLYIFVNPFGGKKIASKIFLDDVKPLLEDANIQFTVQETTQQLHAKEIVKGLDLSKYDGIVCVSGDGILVEVVNGLLEREDWN--------DAIKVP----LGVVPA 715
            F    E    LW + LR  ++S   RPK L +F+NPFGGK    +I+   V PL   A+I   +  T     AKE +  ++   YDGIVCV GDG+  EV++G++ R   +         A+ VP    +G++PA
Sbjct:  104 FWSADEQLCHLWLQTLRGLLESLTSRPKHLLVFINPFGGKGQGKRIYEKTVAPLFTLASITTEIIITEHANQAKETLYEINTDSYDGIVCVGGDGMFSEVLHGVIGRTQQSAGIDPNHPRAVLVPSTLRIGIIPA 238
The following BLAST results are available for this feature:
BLAST of DY306218 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13
Match NameE-valueIdentityDescription
LCB5_YEAST1.647e-1541.90Sphingoid long chain base kinase 5 OS=Saccharomyce... [more]
CERK1_HUMAN4.793e-1537.60Ceramide kinase OS=Homo sapiens GN=CERK PE=1 SV=1[more]
CERK1_MOUSE5.299e-1435.56Ceramide kinase OS=Mus musculus GN=Cerk PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionKN0AAQ11YO19RM1 CitNFL Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY306218 ID=DY306218; Name=DY306218; organism=Citrus clementina; type=EST; length=1276bp
TCCAAGCCTCCGAAAAGAATACCAATTTTAAAATAAAATGATCCTAAACC
CCCCATAAAATCCAAGCCCCAAACCGAACCGAACTCCTTTTCCCCTTCCC
CTGGATCCAATAGTTCCAGACCTACCATCCGACCGGGTCCGTGTTAGCGG
CGGGATAACCGCCATGACGTTGACTGGAGACGGGAGGCCTCGGTGGACGG
ATGGACCCTCACGGAGTTTGACTCTGGAGAAGCAGGTTCTCGGTTTTGTT
GTGGAGGGTAATAAAATCAGAATCAGGGCGGTTGTTGATGGAAGAGATGA
AATCTGCTGCGGCGGGAGCGCGGGAAGCATCGTGAGGAAAGATTTTGTTT
TCCAGCCTTTGTCTGAGGATTCTAAAAGACTCTGGTGCGAAAAACTTCGT
GATTTTATTGATTCTTTTGGTCGTCCGAAGAGGCTATACATTTTTGTGAA
TCCATTTGGTGGGAAGAAAATTGCGTCCAAGATTTTTCTTGATGATGTGA
AACCACTCTTGGAGGATGCCAACATCCAATTTACAGTGCAAGAAACTACG
CAACAACTGCATGCCAAGGAGATTGTCAAAGGGCTGGATCTTTCCAAGTA
TGATGGCATAGTTTGTGTCAGTGGTGATGGTATACTTGTTGAGGTAGTGA
ATGGACTACTTGAGAGAGAGGATTGGAATGATGCAATTAAAGTGCCCCTT
GGAGTGGTTCCTGCANGCACGGGAAATGGCATGATCCAATCCCTACTGGA
TTTGGTTGGTGAACCGTGTAAAGCTTCCATGCCCTTCTAGCTGTTATTCC
AGGTCATAAGCGTCCTCTAGATGTAACTACCCTCCTGCCAGGGCAGACCC
CGATCCCAAATGTCCCGATGCCTGCCGGGGGCCCTGTGGCCCACCTTGTT
ATTGAGTCCGAAAAATATAAATGGAATGGGAATGCCCCCCTTGAATTCCC
AGCCCCTCCCAGGGAAATGTACCCGAGGCCATAAACCCCACCGGTTTCCT
TTGTGCCCGCCCCCGGCCTTAAAAAACCCCCGGAACCCCCCCCCTCTATG
GGAACAAAAATCCCCCCCCCCCCTCCCCCCCCCCCCCCCCCCCCCCTAAA
ATTTGCCCCCCCGGCCCCCCGCGCCCCCCGGTTTTTTTTTTAAAAAAACC
CCAAAGGGGGAAAAAAATTATGGGCCCCCTTTTTTTGTGCCGTGGTGCTG
CCTCCCAACATATACCCGCGGCCGGCCGCCCCCCCAAACACCCCCCCCCC
CCCCCGCCCCCCCCCCCCCCCCCCCC
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