FC868491

Overview
NameFC868491
Unique NameFC868491
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length702
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig9337 contig Ccv1_Contig9337:1..703. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: NTF3_TOBAC (Mitogen-activated protein kinase homolog NTF3 OS=Nicotiana tabacum GN=NTF3 PE=1 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 6.309e-44
Identity = 75/111 (67.57%), Postives = 96/111 (86.49%), Query Frame = 1 
Query:  304 YNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            Y+++ +LFE+ +KYVP I+PIGRGAYGIVC++VN ET E+VAIKKI NAF+N IDA RTLRE+KLLRH+ HENVIA+KD++ P  + +F DVY+VYELMDTDLHQI++S Q
Sbjct:   19 YSMWQSLFEIDTKYVP-IKPIGRGAYGIVCSSVNRETNEKVAIKKINNAFENRIDALRTLRELKLLRHLRHENVIALKDVMMPIHRRSFKDVYLVYELMDTDLHQIIKSSQ 128

HSP 2 Score: 38.891 bits (89), Expect = 6.309e-44
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            DHCQYFL+QLLRG +Y+H
Sbjct:  133 DHCQYFLFQLLRGLKYLH 150
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MPK4_ORYSJ (Mitogen-activated protein kinase 4 OS=Oryza sativa subsp. japonica GN=MPK4 PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 5.246e-43
Identity = 73/111 (65.77%), Postives = 92/111 (82.88%), Query Frame = 1 
Query:  304 YNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            Y ++  LFE+ +KYVP I+PIGRGAYGIVC+++N  T E+VAIKKI N FDN +DA RTLRE+KLLRH+ HENVIA+KDI+ P  + +F DVY+VYELMDTDLHQI++S Q
Sbjct:   19 YTMWQTLFEIDTKYVP-IKPIGRGAYGIVCSSINRATNEKVAIKKINNVFDNRVDALRTLRELKLLRHLRHENVIALKDIMMPVHRRSFKDVYLVYELMDTDLHQIIKSSQ 128

HSP 2 Score: 38.891 bits (89), Expect = 5.246e-43
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            DHCQYFL+QLLRG +Y+H
Sbjct:  133 DHCQYFLFQLLRGLKYLH 150
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MPK1_ARATH (Mitogen-activated protein kinase 1 OS=Arabidopsis thaliana GN=MPK1 PE=1 SV=2)

HSP 1 Score: 153.68 bits (387), Expect = 1.513e-42
Identity = 71/111 (63.96%), Postives = 95/111 (85.59%), Query Frame = 1 
Query:  304 YNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            ++++  LFE+ +KY+P I+PIGRGAYG+VC++VNS+T E+VAIKKI N ++N IDA RTLRE+KLLRH+ HENVIA+KD++ P  K +F DVY+VYELMDTDLHQI++S Q
Sbjct:   19 FSMWQTLFEIDTKYMP-IKPIGRGAYGVVCSSVNSDTNEKVAIKKIHNVYENRIDALRTLRELKLLRHLRHENVIALKDVMMPIHKMSFKDVYLVYELMDTDLHQIIKSSQ 128

HSP 2 Score: 39.6614 bits (91), Expect = 1.513e-42
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            DHCQYFL+QLLRG +Y+H
Sbjct:  133 DHCQYFLFQLLRGLKYIH 150
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MPK7_ARATH (Mitogen-activated protein kinase 7 OS=Arabidopsis thaliana GN=MPK7 PE=2 SV=2)

HSP 1 Score: 155.992 bits (393), Expect = 1.513e-42
Identity = 71/111 (63.96%), Postives = 93/111 (83.78%), Query Frame = 1 
Query:  304 YNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            Y+++  LFE+ +KYVP I+PIGRGAYG+VC+++N ET E VAIKKI N F+N +DA RTLRE+KLLRH+ HENVIA+KD++ P  + +F DVY+VYELMDTDLHQI++S Q
Sbjct:   19 YSMWQTLFEIDTKYVP-IKPIGRGAYGVVCSSINRETNERVAIKKIHNVFENRVDALRTLRELKLLRHVRHENVIALKDVMLPANRSSFKDVYLVYELMDTDLHQIIKSSQ 128

HSP 2 Score: 37.3502 bits (85), Expect = 1.513e-42
Identity = 13/18 (72.22%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            DHC+YFL+QLLRG +Y+H
Sbjct:  133 DHCKYFLFQLLRGLKYLH 150
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MPK2_ARATH (Mitogen-activated protein kinase 2 OS=Arabidopsis thaliana GN=MPK2 PE=1 SV=2)

HSP 1 Score: 150.599 bits (379), Expect = 1.257e-41
Identity = 70/111 (63.06%), Postives = 92/111 (82.88%), Query Frame = 1 
Query:  304 YNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            ++++  LFE+ +KYVP I+PIGRGAYG+VC++VN E+ E VAIKKI N F+N IDA RTLRE+KLLRH+ HENV+A+KD++    K +F DVY+VYELMDTDLHQI++S Q
Sbjct:   19 FSMWQTLFEIDTKYVP-IKPIGRGAYGVVCSSVNRESNERVAIKKIHNVFENRIDALRTLRELKLLRHLRHENVVALKDVMMANHKRSFKDVYLVYELMDTDLHQIIKSSQ 128

HSP 2 Score: 39.6614 bits (91), Expect = 1.257e-41
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            DHCQYFL+QLLRG +Y+H
Sbjct:  133 DHCQYFLFQLLRGLKYIH 150
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MPK14_ARATH (Mitogen-activated protein kinase 14 OS=Arabidopsis thaliana GN=MPK14 PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 4.723e-41
Identity = 70/111 (63.06%), Postives = 93/111 (83.78%), Query Frame = 1 
Query:  304 YNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            Y ++  LFE+ +KYVP I+PIGRGAYG+VC+++NSET E VAIKKI N F+N IDA RTLRE+KLLRH+ HENVI++KD++ P  + +F DVY+VYELMD+DL+QI++S Q
Sbjct:   19 YTMWQTLFEIDTKYVP-IKPIGRGAYGVVCSSINSETNERVAIKKIHNVFENRIDALRTLRELKLLRHVRHENVISLKDVMLPTHRYSFRDVYLVYELMDSDLNQIIKSSQ 128

HSP 2 Score: 37.3502 bits (85), Expect = 4.723e-41
Identity = 13/18 (72.22%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            DHC+YFL+QLLRG +Y+H
Sbjct:  133 DHCKYFLFQLLRGLKYLH 150
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: ERK1_DICDI (Extracellular signal-regulated kinase 1 OS=Dictyostelium discoideum GN=erkA PE=2 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 2.411e-36
Identity = 67/113 (59.29%), Postives = 86/113 (76.11%), Query Frame = 1 
Query:  298 VQYNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            + Y VYG+ F V  +Y   ++ IG GAYG+VC+A ++ T E+VAIKKI  AFDN+ D +RTLREI LLRH +HEN+I+IKDI++P  K+ F DVYIV ELMDTDLHQI+ S Q
Sbjct:  134 ISYFVYGSQFTVPRRY-SIVKCIGHGAYGVVCSAKDNLTGEKVAIKKISKAFDNLKDTKRTLREIHLLRHFKHENLISIKDILKPNSKEQFEDVYIVSELMDTDLHQIITSPQ 245

HSP 2 Score: 37.3502 bits (85), Expect = 2.411e-36
Identity = 12/18 (66.67%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            DHCQYF+YQ+LRG +++H
Sbjct:  250 DHCQYFVYQMLRGLKHIH 267
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MPK17_ORYSJ (Mitogen-activated protein kinase 17 OS=Oryza sativa subsp. japonica GN=MPK17 PE=2 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 2.764e-27
Identity = 46/90 (51.11%), Postives = 72/90 (80.00%), Query Frame = 1 
Query:  364 IGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSD 633
            IG+G+YG+V AAV+++T E VAIKKI + FD++ DA R LREIKLLR + H +++ IK I+ PP +  F D+Y+++ELM++DLHQ+++++
Sbjct:  111 IGKGSYGVVAAAVDTQTGERVAIKKINDVFDHVSDATRILREIKLLRLLRHPDIVEIKHIMLPPSRREFRDIYVIFELMESDLHQVIKAN 200

HSP 2 Score: 33.4982 bits (75), Expect = 2.764e-27
Identity = 12/18 (66.67%), Postives = 16/18 (88.89%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            +H Q+FLYQLLRG +Y+H
Sbjct:  206 EHHQFFLYQLLRGMKYIH 223
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MPK15_ORYSJ (Mitogen-activated protein kinase 15 OS=Oryza sativa subsp. japonica GN=MPK15 PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.771e-27
Identity = 45/90 (50.00%), Postives = 73/90 (81.11%), Query Frame = 1 
Query:  364 IGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPPKKDTFNDVYIVYELMDTDLHQIVRSD 633
            IG+G+YG+VC+A+++ T E+VAIKKI + F+++ DA R LREIKLLR + H +++ IK I+ PP +  F D+Y+V+ELM++DLHQ+++++
Sbjct:   19 IGKGSYGVVCSALDTHTGEKVAIKKINDIFEHVSDATRILREIKLLRLLRHPDIVEIKHILLPPSRREFKDIYVVFELMESDLHQVIKAN 108

HSP 2 Score: 33.8834 bits (76), Expect = 2.771e-27
Identity = 12/18 (66.67%), Postives = 16/18 (88.89%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            +H Q+FLYQLLRG +Y+H
Sbjct:  114 EHYQFFLYQLLRGLKYIH 131
[Close]
BLAST of FC868491 vs. ExPASy Swiss-Prot
Match: MK07_HUMAN (Mitogen-activated protein kinase 7 OS=Homo sapiens GN=MAPK7 PE=1 SV=2)

HSP 1 Score: 108.227 bits (269), Expect = 3.581e-27
Identity = 55/105 (52.38%), Postives = 76/105 (72.38%), Query Frame = 1 
Query:  325 FEVSSKYVPPIRPIGRGAYGIVCAAVNSETREEVAIKKIGNAFDNIIDARRTLREIKLLRHMEHENVIAIKDIIRPP-KKDTFNDVYIVYELMDTDLHQIVRSDQ 636
            F+V  +Y   I  IG GAYG+V +A    T ++VAIKKI NAFD + +A+RTLRE+K+L+H +H+N+IAIKDI+RP      F  VY+V +LM++DLHQI+ S Q
Sbjct:   49 FDVGDEY-EIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQ 152

HSP 2 Score: 33.4982 bits (75), Expect = 3.581e-27
Identity = 12/18 (66.67%), Postives = 16/18 (88.89%), Query Frame = 3 
Query:  648 DHCQYFLYQLLRGFEYVH 701
            +H +YFLYQLLRG +Y+H
Sbjct:  157 EHVRYFLYQLLRGLKYMH 174
The following BLAST results are available for this feature:
BLAST of FC868491 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 189
Match NameE-valueIdentityDescription
NTF3_TOBAC6.309e-4467.57Mitogen-activated protein kinase homolog NTF3 OS=N... [more]
MPK4_ORYSJ5.246e-4365.77Mitogen-activated protein kinase 4 OS=Oryza sativa... [more]
MPK1_ARATH1.513e-4263.96Mitogen-activated protein kinase 1 OS=Arabidopsis ... [more]
MPK7_ARATH1.513e-4263.96Mitogen-activated protein kinase 7 OS=Arabidopsis ... [more]
MPK2_ARATH1.257e-4163.06Mitogen-activated protein kinase 2 OS=Arabidopsis ... [more]
MPK14_ARATH4.723e-4163.06Mitogen-activated protein kinase 14 OS=Arabidopsis... [more]
ERK1_DICDI2.411e-3659.29Extracellular signal-regulated kinase 1 OS=Dictyos... [more]
MPK17_ORYSJ2.764e-2751.11Mitogen-activated protein kinase 17 OS=Oryza sativ... [more]
MPK15_ORYSJ2.771e-2750.00Mitogen-activated protein kinase 15 OS=Oryza sativ... [more]
MK07_HUMAN3.581e-2752.38Mitogen-activated protein kinase 7 OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionC31001A06EF AbioticL1 Citrus clementina cDNA clone C31001A06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC868491 ID=FC868491; Name=FC868491; organism=Citrus clementina; type=EST; length=702bp
CTTCCCGGGGACGGATTTTCAAACAAATTTCATTACCAAAGGTGTGGTTA
CGAGGCGCAAAAAGTTGTTGCTGCATTCAAATTTTCACATTCACAACACG
CTCTGGCAAGACCCAAATGCTCAAGCTCAACCAAACCGAGCAAGCTCAAC
TCTGATAAAACAGTAAACGGCCAGAAGCAGAGAAAAAGAGAACCCAGTTC
TGGGTTTTGAGTGAAAATGGCAGCAGCGGCGAAGGATTCGAGTTCAGGTG
GTGCGACTAGCAGTGTCAAAGGGGTGCTCACACATGGGGGTAAATACGTA
CAGTACAATGTTTACGGTAACCTTTTTGAGGTTTCTAGCAAGTATGTCCC
TCCCATTCGACCCATTGGTAGGGGTGCTTATGGCATTGTTTGCGCTGCTG
TAAATTCAGAGACACGCGAGGAGGTTGCCATTAAGAAGATCGGTAATGCA
TTTGACAACATAATAGATGCCAGAAGGACCTTGAGAGAAATTAAGCTTCT
TCGCCACATGGAGCATGAAAATGTCATCGCCATTAAGGATATCATTCGGC
CTCCCAAAAAGGACACCTTTAATGATGTATACATTGTTTATGAATTGATG
GACACTGATCTTCACCAGATAGTTCGCTCTGACCAACACTAACGGATGAT
CATTGTCAGTACTTTTTATATCAGTTGTTACGAGGATTTGAATATGTACA
TT
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