FC869243

Overview
NameFC869243
Unique NameFC869243
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length596
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: ROCA_STAA8 (1-pyrroline-5-carboxylate dehydrogenase OS=Staphylococcus aureus (strain NCTC 8325) GN=rocA PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.047e-24
Identity = 64/168 (38.10%), Postives = 87/168 (51.79%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAA-----KSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKA 489
            G    I PWNFP  +        +  GNT++LK AE TP  A  + ++  EAGLP GV+N V G     G  L  H D   ++FTG   TG  + E +A     ++ LK V  E+GGK   ++ ++ D D A E    + F   GQ C A SR  VH+ VYDE +EK+
Sbjct:  173 GTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKS 340

HSP 2 Score: 22.3274 bits (46), Expect = 1.047e-24
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 2 
Query:  545 GPQIDPEQFEKVLKYI 592
            GP I+ +QF+K+  YI
Sbjct:  359 GPVINKKQFDKIKNYI 374
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: ROCA_STAA3 (1-pyrroline-5-carboxylate dehydrogenase OS=Staphylococcus aureus (strain USA300) GN=rocA PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.047e-24
Identity = 64/168 (38.10%), Postives = 87/168 (51.79%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAA-----KSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKA 489
            G    I PWNFP  +        +  GNT++LK AE TP  A  + ++  EAGLP GV+N V G     G  L  H D   ++FTG   TG  + E +A     ++ LK V  E+GGK   ++ ++ D D A E    + F   GQ C A SR  VH+ VYDE +EK+
Sbjct:  173 GTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKS 340

HSP 2 Score: 22.3274 bits (46), Expect = 1.047e-24
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 2 
Query:  545 GPQIDPEQFEKVLKYI 592
            GP I+ +QF+K+  YI
Sbjct:  359 GPVINKKQFDKIKNYI 374
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: ROCA_STAA2 (1-pyrroline-5-carboxylate dehydrogenase OS=Staphylococcus aureus (strain JH1) GN=rocA PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.047e-24
Identity = 64/168 (38.10%), Postives = 87/168 (51.79%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAA-----KSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKA 489
            G    I PWNFP  +        +  GNT++LK AE TP  A  + ++  EAGLP GV+N V G     G  L  H D   ++FTG   TG  + E +A     ++ LK V  E+GGK   ++ ++ D D A E    + F   GQ C A SR  VH+ VYDE +EK+
Sbjct:  173 GTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKS 340

HSP 2 Score: 22.3274 bits (46), Expect = 1.047e-24
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 2 
Query:  545 GPQIDPEQFEKVLKYI 592
            GP I+ +QF+K+  YI
Sbjct:  359 GPVINKKQFDKIKNYI 374
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: ROCA_STAA1 (1-pyrroline-5-carboxylate dehydrogenase OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) GN=rocA PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.047e-24
Identity = 64/168 (38.10%), Postives = 87/168 (51.79%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAA-----KSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKA 489
            G    I PWNFP  +        +  GNT++LK AE TP  A  + ++  EAGLP GV+N V G     G  L  H D   ++FTG   TG  + E +A     ++ LK V  E+GGK   ++ ++ D D A E    + F   GQ C A SR  VH+ VYDE +EK+
Sbjct:  173 GTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKS 340

HSP 2 Score: 22.3274 bits (46), Expect = 1.047e-24
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 2 
Query:  545 GPQIDPEQFEKVLKYI 592
            GP I+ +QF+K+  YI
Sbjct:  359 GPVINKKQFDKIKNYI 374
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: ROCA_STAES (1-pyrroline-5-carboxylate dehydrogenase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=rocA PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.363e-24
Identity = 65/168 (38.69%), Postives = 87/168 (51.79%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAA-----KSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKA 489
            G    I PWNFP  +        +  GNT++LK AE T LTA  + ++  EAGLP GV+N V G     G  L  H D   ++FTG   TG  + E +A     +  LK V  E+GGK   ++ ++ D D A E    + F   GQ C A SR  VH+ V+DE +EKA
Sbjct:  173 GTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTVLTAYKLMEILEEAGLPQGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQQFLKRVIAEMGGKDAIVVDNNVDTDLAAEAIVTSAFGFSGQKCSACSRAIVHQDVHDEILEKA 340

HSP 2 Score: 22.3274 bits (46), Expect = 1.363e-24
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 2 
Query:  545 GPQIDPEQFEKVLKYI 592
            GP I+ +QF+K+  YI
Sbjct:  359 GPVINQKQFDKIKNYI 374
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: ROCA_STAEQ (1-pyrroline-5-carboxylate dehydrogenase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=rocA PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.363e-24
Identity = 65/168 (38.69%), Postives = 87/168 (51.79%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAA-----KSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKA 489
            G    I PWNFP  +        +  GNT++LK AE T LTA  + ++  EAGLP GV+N V G     G  L  H D   ++FTG   TG  + E +A     +  LK V  E+GGK   ++ ++ D D A E    + F   GQ C A SR  VH+ V+DE +EKA
Sbjct:  173 GTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTVLTAYKLMEILEEAGLPQGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQQFLKRVIAEMGGKDAIVVDNNVDTDLAAEAIVTSAFGFSGQKCSACSRAIVHQDVHDEILEKA 340

HSP 2 Score: 22.3274 bits (46), Expect = 1.363e-24
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 2 
Query:  545 GPQIDPEQFEKVLKYI 592
            GP I+ +QF+K+  YI
Sbjct:  359 GPVINQKQFDKIKNYI 374
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: GAPN_STREI (NADP-dependent glyceraldehyde-3-phosphate dehydrogenase OS=Streptococcus equinus GN=gapN PE=1 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.674e-24
Identity = 63/177 (35.59%), Postives = 96/177 (54.24%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKARHVQ*GVLLAIPSE 531
            G+   I P+N+P+ L   K+APAL  GN + LK   Q  ++ L +A+ F EAGLP GV N ++G G   G  +  H  V+ ++FTG    G+ +  LA    ++P+ LELGGK   II +DAD+D A +      +   GQ C A  R  V + + D+ VEK   +   + + +P +
Sbjct:  145 GLVLAISPFNYPINLAGSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGLPAGVFNTITGRGSVIGDYIVEHEAVNYINFTGSTPVGEHIGHLA---GMRPIMLELGGKDSAIILEDADLDLAAKNIVAGAYGYSGQRCTAVKRVLVMDSIADKLVEKVSALVNNLTVGMPED 318
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: ASTD_NOVAD (N-succinylglutamate 5-semialdehyde dehydrogenase OS=Novosphingobium aromaticivorans (strain DSM 12444) GN=astD PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.674e-24
Identity = 58/175 (33.14%), Postives = 93/175 (53.14%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKARHVQ*GVLLAIP 525
            GV   + P+NFP  L    + PAL  GN +V K +E+TP T   +A+ FH AG+P  V+ V+ G GP  G AL +H  +D + FTG    G  +    A +  K V LE+GG +P +++D   ++ A  L   + F + GQ C A  R  +   ++DE ++  + +   +++  P
Sbjct:  123 GVLAVLGPYNFPAHLPNGHIVPALIAGNAVVFKPSEKTPATGEMLAQCFHRAGIPAAVVQVLIG-GPEEGQALVAHDGIDGVLFTGSAHAGIAINRKLASNPGKIVALEMGGNNPIVVWDTPKIEDAATLIVQSAFTSAGQRCTAARRLIIKASMFDEVIDHVKRLADRIIVGAP 296
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: LADH_METM5 (Lactaldehyde dehydrogenase OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=MmarC5_0090 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.186e-24
Identity = 55/165 (33.33%), Postives = 94/165 (56.97%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFH----EAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVE 483
            GV G I P+NFPL L T K+ PA+  GN++VL  + + P+ A+Y+ K+      +  +P G+ N+ +G G   G  ++ + +++ +SFTG  + G   + ++  + +K V LELGG +P I+  D+D+  A + A  + F N GQ C +  +  V E V + F +
Sbjct:  131 GVVGAITPFNFPLNLITHKIGPAIATGNSVVLHPSSKAPIVAIYLTKIIEHVLKQMDVPRGIFNLATGNGDIVGDEISKNDNINMVSFTGSVEVG---ESISKNAKMKKVALELGGNNPMIVLKDSDIKLAAKSAVKSKFLNAGQVCISVGQVLVEEEVLETFTK 292
[Close]
BLAST of FC869243 vs. ExPASy Swiss-Prot
Match: GABD2_MYCTU (Putative succinate-semialdehyde dehydrogenase [NADP+] 2 OS=Mycobacterium tuberculosis GN=gabD2 PE=1 SV=3)

HSP 1 Score: 112.079 bits (279), Expect = 2.186e-24
Identity = 65/162 (40.12%), Postives = 87/162 (53.70%), Query Frame = 1 
Query:    1 GVAGQIVPWNFPLLLFTWKVAPALTCGNTIVLKSAEQTPLTALYVAKLFHEAGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEK 486
            GV G I PWN+P+ L      PAL  GN +VLK   QTP  AL  A+L + AGLP  +  +V G G   G A+  + D   L FTG   TG  + E A +  L   + ELGGK+P I+   A++D+  + A  A F N GQ C +  R YV + + +EF  K
Sbjct:  149 GVVGVISPWNYPMTLTVSDSVPALVAGNAVVLKPDSQTPYCALACAELLYRAGLPRALYAIVPGPGSVVGTAITDNCDY--LMFTGSSATGSRLAEHAGR-RLIGFSAELGGKNPMIVARGANLDKVAKAATRACFSNAGQLCISIERIYVEKDIAEEFTRK 307
The following BLAST results are available for this feature:
BLAST of FC869243 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ROCA_STAA81.047e-2438.101-pyrroline-5-carboxylate dehydrogenase OS=Staphyl... [more]
ROCA_STAA31.047e-2438.101-pyrroline-5-carboxylate dehydrogenase OS=Staphyl... [more]
ROCA_STAA21.047e-2438.101-pyrroline-5-carboxylate dehydrogenase OS=Staphyl... [more]
ROCA_STAA11.047e-2438.101-pyrroline-5-carboxylate dehydrogenase OS=Staphyl... [more]
ROCA_STAES1.363e-2438.691-pyrroline-5-carboxylate dehydrogenase OS=Staphyl... [more]
ROCA_STAEQ1.363e-2438.691-pyrroline-5-carboxylate dehydrogenase OS=Staphyl... [more]
GAPN_STREI1.674e-2435.59NADP-dependent glyceraldehyde-3-phosphate dehydrog... [more]
ASTD_NOVAD1.674e-2433.14N-succinylglutamate 5-semialdehyde dehydrogenase O... [more]
LADH_METM52.186e-2433.33Lactaldehyde dehydrogenase OS=Methanococcus maripa... [more]
GABD2_MYCTU2.186e-2440.12Putative succinate-semialdehyde dehydrogenase [NAD... [more]

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Properties
Property NameValue
Genbank descriptionC31010B01EF AbioticL1 Citrus clementina cDNA clone C31010B01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC869243 ID=FC869243; Name=FC869243; organism=Citrus clementina; type=EST; length=596bp
GGCGTTGCTGGCCAGATAGTTCCCTGGAACTTTCCTCTTCTCTTGTTTAC
TTGGAAAGTAGCGCCTGCACTAACTTGTGGCAATACTATTGTTCTAAAGT
CTGCTGAGCAAACGCCTTTGACTGCTCTGTATGTGGCAAAGCTATTCCAT
GAGGCTGGTCTTCCCCCCGGTGTTTTGAATGTAGTCTCTGGTTATGGTCC
AACAGCTGGTGCAGCTCTTGCCAGCCATATGGATGTGGACAAGCTGTCTT
TTACAGGACACTGTGATACTGGTAAGATTGTTCAGGAACTGGCTGCAAAA
AGCAACCTTAAGCCAGTAACCTTAGAACTTGGAGGGAAATCACCATTTAT
AATATTCGATGATGCTGATGTTGACCAGGCTGTGGAACTTGCACACTTTG
CTTTGTTCTATAATCAGGGACAATGTTGCTGTGCTGGATCTCGTACTTAT
GTACATGAGCGTGTATATGACGAGTTTGTAGAAAAAGCAAGGCACGTGCA
ATGAGGCGTATTGTTGGCGATCCCTTCAGAGTGGTGTTGAACAAGGTCCT
CAGATTGATCCAGAGCAATTTGAGAAGGTCCTTAAGTACATAAGGT
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