FC875689

Overview
NameFC875689
Unique NameFC875689
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length712
Libraries
Library NameType
CTVClemen1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig14122 contig Ccv1_Contig14122:46..758. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_SOLLC (Lactoylglutathione lyase OS=Solanum lycopersicum GN=GLX1 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.864e-13
Identity = 49/148 (33.11%), Postives = 73/148 (49.32%), Query Frame = 3
Query:  114 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQS---------------HFVVELTYNYGV------TSYDIGT----GFGHFAIATEDVYKLVENIRAKGGNVTREP--GPVKGGTTHIAFVKDPDGYIFEL 476
            L   ++R+ D   +++FY++  GM LL++ D PE K+S  F+G+    S                  +ELT+N+G       T Y  G     GFGH  +  +DVYK  E   + G    ++P  G +KG    IAF+KDPDGY  E+
Sbjct:   28 LQQTMFRIKDPKVSLEFYSKVLGMSLLKRLDFPEMKFSLYFMGYEDTASAPSDPVERTAWTFSQKSTLELTHNWGTESDPNFTGYHNGNSEPRGFGHIGVTVDDVYKACERFESLGVEFVKKPLDGKMKG----IAFIKDPDGYWIEI 171          
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_CICAR (Lactoylglutathione lyase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 9.250e-13
Identity = 50/147 (34.01%), Postives = 70/147 (47.62%), Query Frame = 3
Query:  126 VYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGF-----GPEQ----------SHFVVELTYNYGVTS------YDIGT----GFGHFAIATEDVYKLVENIRAKGGNVTREP--GPVKGGTTHIAFVKDPDGYIFELIQR 485
            ++R+ D   ++ FY+   GM LL++ D PE K+S  F+G+      P                +ELT+N+G  S      Y  G     GFGH  I  +D YK  E  +  G    ++P  G +KG    IAF+KDPDGY  EL  R
Sbjct:   33 MFRIKDPKVSLDFYSRVLGMSLLKRLDFPEMKFSLYFMGYEDTTEAPSNPVDRTVWTFAQKATIELTHNWGTESDPEFKGYHNGNSDPRGFGHIGITVDDTYKACERFQNLGVEFVKKPDDGKMKG----IAFIKDPDGYWIELFDR 175          
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_SOYBN (Lactoylglutathione lyase OS=Glycine max GN=GLXI PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.061e-12
Identity = 48/147 (32.65%), Postives = 70/147 (47.62%), Query Frame = 3
Query:  126 VYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGF-----GPE----------QSHFVVELTYNYGVTS------YDIGT----GFGHFAIATEDVYKLVENIRAKGGNVTREP--GPVKGGTTHIAFVKDPDGYIFELIQR 485
            ++R+ D   ++ FY+   GM LL++ D PE K+S  F+G+      P                +ELT+N+G  S      Y  G     GFGH  +  +D YK  E  +  G    ++P  G +KG    IAF+KDPDGY  E+  R
Sbjct:   32 MFRIKDPKVSLDFYSRVLGMSLLKRLDFPEMKFSLYFMGYENTAEAPSNPIDKVVWTFSQKATIELTHNWGTESDPEFKGYHNGNSEPRGFGHIGVTVDDTYKACERFQNLGVEFVKKPEDGKMKG----IAFIKDPDGYWIEIFDR 174          
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_PSEPU (Lactoylglutathione lyase OS=Pseudomonas putida GN=gloA PE=1 SV=3)

HSP 1 Score: 72.0182 bits (175), Expect = 3.515e-12
Identity = 52/150 (34.67%), Postives = 71/150 (47.33%), Query Frame = 3
Query:  120 HAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGF--------GPEQSH-------FVVELTYNYGVT-----SYDIGT----GFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTP 497
            H + RV D+++++ FYT   G KL+ KRD  E K+S  FL            +  H        V+ELT+N+G       +Y  G     GFGH  ++  DV    E   A    V  +     G   H+AF+KDPDGY  E+IQ  PTP
Sbjct:   27 HTMLRVKDIEKSLDFYTRVLGFKLVDKRDFVEAKFSLYFLALVDPATIPADDDARHQWMKSIPGVLELTHNHGTERDADFAYHHGNTDPRGFGHICVSVPDVVAACERFEAL--QVPFQKRLSDGRMNHLAFIKDPDGYWVEVIQ--PTP 172          
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_BRAJU (Lactoylglutathione lyase OS=Brassica juncea GN=GLY I PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.976e-11
Identity = 49/151 (32.45%), Postives = 70/151 (46.36%), Query Frame = 3
Query:  126 VYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQS---------------HFVVELTYNYGVTS------YDIGT----GFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGY---IFELIQRGPT 494
            ++RV D   ++ FY+   GM LL++ D  E K+S  FLG+    +                  +ELT+N+G  S      Y  G     GFGH  +  +DV+K  E     G    ++P    G   +IAF+KDPDGY   IF+L   G T
Sbjct:   32 MFRVKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDTSTAPTDPTERTVWTFGRPATIELTHNWGTESDPEFKGYHNGNSEPRGFGHIGVTVDDVHKACERFEQLGVEFVKKPH--DGKMKNIAFIKDPDGYWIEIFDLKTIGTT 180          
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_ARATH (Lactoylglutathione lyase OS=Arabidopsis thaliana GN=At1g08110 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.976e-11
Identity = 49/151 (32.45%), Postives = 71/151 (47.02%), Query Frame = 3
Query:  126 VYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGF--------GPEQ-------SHFVVELTYNYGVTS------YDIGT----GFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGY---IFELIQRGPT 494
            ++R+ D   ++ FY+   GM LL++ D  E K+S  FLG+         P +           +ELT+N+G  S      Y  G     GFGH  +  +DV+K  E     G    ++P    G   +IAF+KDPDGY   IF+L   G T
Sbjct:   32 MFRIKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDTTTAPTDPTERTVWTFGQPATIELTHNWGTESDPEFKGYHNGNSEPRGFGHIGVTVDDVHKACERFEELGVEFAKKPN--DGKMKNIAFIKDPDGYWIEIFDLKTIGTT 180          
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_PSEAE (Lactoylglutathione lyase OS=Pseudomonas aeruginosa GN=gloA PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 5.076e-11
Identity = 46/147 (31.29%), Postives = 67/147 (45.58%), Query Frame = 3
Query:  120 HAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLG------------------FGPEQSHFVVELTYNYGVTSYDI--------GTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            H + RV D  R++ FY+   GM+LLR+ D  E ++S  FL                   FG +    V+ELT+N+G  S D           GFGH   +  D+    E     G N  +   P+  G  ++AF+ DPDGY  E++Q
Sbjct:   26 HTMLRVKDPKRSLDFYSRVLGMRLLRRLDFEEGRFSLYFLAMTRGEEVPDAVDERQRYTFGRQS---VLELTHNWGSESDDSQYHNGNQDPRGFGHICFSVPDLVAACERFETLGVNFVK---PLDRGMKNVAFISDPDGYWVEIVQ 166          
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_RAT (Lactoylglutathione lyase OS=Rattus norvegicus GN=Glo1 PE=1 SV=3)

HSP 1 Score: 67.781 bits (164), Expect = 6.629e-11
Identity = 47/148 (31.76%), Postives = 69/148 (46.62%), Query Frame = 3
Query:  114 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQS---------------HFVVELTYNYG-----VTSYDIGT----GFGHFAIATEDVYKLVENIRAKGGNVTREP--GPVKGGTTHIAFVKDPDGYIFELI 479
            L   + R+ D  +++ FYT   G+ LL+K D P  K+S  FL +  +                    +ELT+N+G       SY  G     GFGH  IA  DVY+  +     G    ++P  G +KG    +AFV+DPDGY  E++
Sbjct:   32 LQQTMLRIKDPKKSLDFYTRVLGLTLLQKLDFPSMKFSLYFLAYEDKNDIPKDKTERTAWAFSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYEACKRFEELGVKFVKKPDDGKMKG----LAFVQDPDGYWIEIL 175          
The following BLAST results are available for this feature:
BLAST of FC875689 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
LGUL_SOLLC1.864e-1333.11Lactoylglutathione lyase OS=Solanum lycopersicum G... [more]
LGUL_CICAR9.250e-1334.01Lactoylglutathione lyase OS=Cicer arietinum PE=2 S... [more]
LGUL_SOYBN2.061e-1232.65Lactoylglutathione lyase OS=Glycine max GN=GLXI PE... [more]
LGUL_PSEPU3.515e-1234.67Lactoylglutathione lyase OS=Pseudomonas putida GN=... [more]
LGUL_BRAJU2.976e-1132.45Lactoylglutathione lyase OS=Brassica juncea GN=GLY... [more]
LGUL_ARATH2.976e-1132.45Lactoylglutathione lyase OS=Arabidopsis thaliana G... [more]
LGUL_PSEAE5.076e-1131.29Lactoylglutathione lyase OS=Pseudomonas aeruginosa... [more]
LGUL_RAT6.629e-1131.76Lactoylglutathione lyase OS=Rattus norvegicus GN=G... [more]

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Properties
Property NameValue
Genbank descriptionC31401G03EF CTVClemen1 Citrus clementina cDNA clone C31401G03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC875689 ID=FC875689; Name=FC875689; organism=Citrus clementina; type=EST; length=712bp
GACTTGCGACTTCACTCCGACTTTCTGAGTGATTCCATATCAAAATGGCT
GAGGCTTCTCCTGCTGCTGCTAATGCCGAGTTGCTGGAATGGCCAAAGAA
AGATAAGCGACGCCTCCTTCATGCTGTGTATCGTGTTGGTGATCTTGATC
GCACCATCAAATTTTACACCGAATGCTTTGGGATGAAGCTGTTGAGAAAG
AGAGACGTCCCGGAAGAGAAATACTCAAATGCCTTTCTTGGATTTGGCCC
CGAACAGTCTCACTTTGTTGTGGAGTTAACGTACAACTATGGTGTAACTT
CATATGATATTGGAACTGGTTTTGGCCATTTTGCAATTGCAACTGAAGAT
GTTTACAAACTGGTTGAAAACATTCGTGCCAAGGGTGGCAATGTCACTAG
AGAGCCTGGTCCAGTTAAAGGTGGAACAACTCACATTGCCTTTGTGAAGG
ATCCCGATGGTTATATTTTTGAACTCATCCAGAGAGGTCCAACTCCTGAA
CCACTCTGTCAAGTAATGCTTCGCGTTGGCGATCTTGGTCGCTCCATCAA
GTTCTATGAAAAGGCTTTGGGGATGAAACTGTTAAGGACAGTTGATAAAC
CTGAATACAAGTACACCCTGGCCATGCTTGGATATGCTGAGGAAGATCAG
ACTACTGTTCTGGAGTTAACTTATAATTATGGGGTGACTGAATATACCAG
GGNAATGCATAT
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