FC876300

Overview
NameFC876300
Unique NameFC876300
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length558
Libraries
Library NameType
DevOvary1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: IF4A_LODEL (ATP-dependent RNA helicase eIF4A OS=Lodderomyces elongisporus GN=TIF1 PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 2.893e-57
Identity = 110/184 (59.78%), Postives = 139/184 (75.54%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG  ATF    LQ++D      QAL+LAPTRELA QI+ V+ ++G YL V VHA +GGTS+ +D    ++GV +VVGTPGRVFDM+ R+  R D +KMF+LDEADEMLS GFK+QIY+IF+LLP   QV + SATMP + LE+T KFMN P RILVK DELTLEGIKQ+++ VE E++K +
Sbjct:   68 QSGTGKTATFTISALQRIDENEKSTQALILAPTRELALQIKNVITSIGLYLNVTVHASIGGTSMSDDIEAFKSGVQIVVGTPGRVFDMIERRYFRTDKVKMFILDEADEMLSSGFKEQIYNIFRLLPETTQVVLLSATMPQDVLEVTTKFMNNPVRILVKKDELTLEGIKQYFINVEVEDYKFD 251
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: IF4A3_NICPL (Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 6.444e-57
Identity = 110/184 (59.78%), Postives = 139/184 (75.54%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG  +     + Q +D    + QAL+L+PTRELA Q EKV+ A+GDY+ V+ HAC+GG SV ED R L+ GV VV GTPGRV DM++R++LR   IK+ +LDE+DEMLSRGFKDQIYD+++ LP ++QV + SAT+P E LEIT KFM  P RILVK DELTLEGIKQF++ VE EEWK +
Sbjct:   63 QSGTGKTSMIALTVCQIVDTKSSEVQALILSPTRELAAQTEKVILAIGDYINVQAHACIGGKSVGEDIRKLEHGVQVVSGTPGRVCDMIKRRTLRTRGIKLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPNEILEITSKFMTDPVRILVKRDELTLEGIKQFFVAVEKEEWKFD 246
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: IF4A_CANAL (ATP-dependent RNA helicase eIF4A OS=Candida albicans GN=TIF1 PE=3 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 2.449e-56
Identity = 109/184 (59.24%), Postives = 138/184 (75.00%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG  ATF    LQ+++      QAL+LAPTRELA QI+ V+ A+G YL V VHA +GGTS+ +D    ++GV +VVGTPGRV DM+ R+  + D +KMF+LDEADEMLS GFK+QIY+IF+LLP   Q+ + SATMP + LE+T KFMN P RILVK DELTLEGIKQFY+ VE E++K +
Sbjct:   68 QSGTGKTATFTISALQRINENEKATQALILAPTRELALQIKNVITAIGLYLKVTVHASIGGTSMSDDIEAFRSGVQIVVGTPGRVLDMIERRYFKTDKVKMFILDEADEMLSSGFKEQIYNIFRLLPETTQIVLLSATMPQDVLEVTTKFMNNPVRILVKKDELTLEGIKQFYINVELEDYKFD 251
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: IF4A_KLULA (ATP-dependent RNA helicase eIF4A OS=Kluyveromyces lactis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 3.198e-56
Identity = 108/184 (58.70%), Postives = 136/184 (73.91%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG   TF    LQ +D  +   Q L+LAPTRELA QI+KV+ AL  ++ VKVHAC+GGTS++ED   L+ G  ++VGTPGRVFDM+ R+  + D IKMF+LDEADEMLS GFK+QIY+IF +LP   QV + SATMP + LE+T KFM  P RILVK DELTLEGI Q+Y+ VE E++K +
Sbjct:   67 QSGTGKTGTFSIAALQNIDEKIKAPQGLILAPTRELALQIQKVVMALAIHMDVKVHACIGGTSLQEDSEALRGGAQIIVGTPGRVFDMIDRRIFKTDNIKMFILDEADEMLSTGFKEQIYNIFTMLPPTSQVVLLSATMPGDVLEVTSKFMKDPVRILVKKDELTLEGIGQYYVNVEEEQYKYD 250
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: RH2_ARATH (DEAD-box ATP-dependent RNA helicase 2 OS=Arabidopsis thaliana GN=RH2 PE=2 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 9.305e-56
Identity = 106/184 (57.61%), Postives = 138/184 (75.00%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG  +     + Q +D    + QAL+L+PTRELA Q EK ++A+G +  ++ HAC+GG SV ED R L+ GVHVV GTPGRV DM++R+SLR   IK+ +LDE+DEMLSRGFKDQIYD+++ LP  +QV + SAT+P E LE+T KFM +P +ILVK DELTLEGIKQF++ VE EEWK +
Sbjct:   80 QSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHANIQAHACIGGNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLSRGFKDQIYDVYRYLPPDLQVCLVSATLPHEILEMTSKFMTEPVKILVKRDELTLEGIKQFFVAVEKEEWKFD 263
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: IF4A_YEAST (ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae GN=TIF1 PE=1 SV=3)

HSP 1 Score: 215.698 bits (548), Expect = 1.215e-55
Identity = 113/184 (61.41%), Postives = 135/184 (73.37%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG   TF    LQ++D  +   QAL+LAPTRELA QI+KV+ AL  ++ +KVHAC+GGTS  ED   L+    +VVGTPGRVFD ++R+  R D IKMF+LDEADEMLS GFK+QIY IF LLP   QV + SATMP + LE+T KFM  P RILVK DELTLEGIKQFY+ VE EE+K E
Sbjct:   67 QSGTGKTGTFSIAALQRIDTSVKAPQALMLAPTRELALQIQKVVMALAFHMDIKVHACIGGTSFVEDAEGLR-DAQIVVGTPGRVFDNIQRRRFRTDKIKMFILDEADEMLSSGFKEQIYQIFTLLPPTTQVVLLSATMPNDVLEVTTKFMRNPVRILVKKDELTLEGIKQFYVNVEEEEYKYE 249
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: IF4A_YEAS7 (ATP-dependent RNA helicase eIF4A OS=Saccharomyces cerevisiae (strain YJM789) GN=TIF1 PE=3 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.215e-55
Identity = 113/184 (61.41%), Postives = 135/184 (73.37%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG   TF    LQ++D  +   QAL+LAPTRELA QI+KV+ AL  ++ +KVHAC+GGTS  ED   L+    +VVGTPGRVFD ++R+  R D IKMF+LDEADEMLS GFK+QIY IF LLP   QV + SATMP + LE+T KFM  P RILVK DELTLEGIKQFY+ VE EE+K E
Sbjct:   67 QSGTGKTGTFSIAALQRIDTSVKAPQALMLAPTRELALQIQKVVMALAFHMDIKVHACIGGTSFVEDAEGLR-DAQIVVGTPGRVFDNIQRRRFRTDKIKMFILDEADEMLSSGFKEQIYQIFTLLPPTTQVVLLSATMPNDVLEVTTKFMRNPVRILVKKDELTLEGIKQFYVNVEEEEYKYE 249
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: IF4A_CANGA (ATP-dependent RNA helicase eIF4A OS=Candida glabrata GN=TIF1 PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 6.031e-55
Identity = 110/184 (59.78%), Postives = 136/184 (73.91%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG   TF    LQ++D  +   QAL+LAPTRELA QI+KV+ ALG ++ +KVHAC+GGTS  ED   L+    +VVGTPGRVFD ++R+  + D IKMF+LDEADEMLS GFK+QIY IF +LP   QV + SATMP + LE+T KFM  P RILVK DELTLEGIKQFY+ VE E++K +
Sbjct:   68 QSGTGKTGTFSIAALQRIDPAIKAPQALMLAPTRELALQIQKVVMALGFHMDIKVHACIGGTSFVEDAEGLR-DAQIVVGTPGRVFDNIQRRKFKVDNIKMFILDEADEMLSTGFKEQIYQIFTMLPPTTQVVLLSATMPRDVLEVTAKFMQNPVRILVKKDELTLEGIKQFYVNVEEEQFKYD 250
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: FAL1_YARLI (ATP-dependent RNA helicase FAL1 OS=Yarrowia lipolytica GN=FAL1 PE=3 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 7.877e-55
Identity = 104/184 (56.52%), Postives = 137/184 (74.46%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG  ATF   +L+ +D    + QA+VL+PTRELA QI+ V+ ALGDY+ V+ HAC+GGTS+  D + L+AG  VV GTPGR  DM+++  LR   +KM +LDEADE+L++GF++QIYDI++ LPA  QV V SAT+P   LE+T KFM  P RILVK DELTLEG+KQ+++ VE EEWK +
Sbjct:   69 QSGTGKTATFSISMLEVIDTKHRETQAMVLSPTRELATQIQSVILALGDYMNVQCHACIGGTSLSVDMKKLEAGQQVVSGTPGRCLDMIKKGCLRTKNLKMLILDEADELLNKGFQEQIYDIYRYLPAATQVVVVSATLPHSVLEMTSKFMTDPVRILVKRDELTLEGLKQYFIAVEQEEWKFD 252
[Close]
BLAST of FC876300 vs. ExPASy Swiss-Prot
Match: FAL1_DEBHA (ATP-dependent RNA helicase FAL1 OS=Debaryomyces hansenii GN=FAL1 PE=3 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.755e-54
Identity = 102/184 (55.43%), Postives = 137/184 (74.46%), Query Frame = 3 
Query:    6 QSGTGNPATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGVFSATMPPEALEITRKFMNKPARILVKLDELTLEGIKQFYLTVENEEWKLE 557
            QSGTG  ATF  G+L+ +D     CQAL+L+PTRELAQQI+ V++ LGDY+ V  HAC+GGT V ED + LQ G  +V GTPGRV DM++R++L    IKM +LDEADE++++GFK+QIY+I++ LP  VQV V SAT+  E LE+T KF   P +ILVK D++TLEGIKQ+++  E E+WK +
Sbjct:   71 QSGTGKTATFSIGMLEVIDTKSKDCQALILSPTRELAQQIQSVVKHLGDYMNVHTHACIGGTHVGEDIKKLQQGQQIVSGTPGRVVDMIKRRNLATRNIKMMILDEADELMTKGFKEQIYEIYRYLPPGVQVVVVSATLSREVLEVTGKFTTDPVKILVKRDDITLEGIKQYHIQCEKEDWKFD 254
The following BLAST results are available for this feature:
BLAST of FC876300 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A_LODEL2.893e-5759.78ATP-dependent RNA helicase eIF4A OS=Lodderomyces e... [more]
IF4A3_NICPL6.444e-5759.78Eukaryotic initiation factor 4A-3 OS=Nicotiana plu... [more]
IF4A_CANAL2.449e-5659.24ATP-dependent RNA helicase eIF4A OS=Candida albica... [more]
IF4A_KLULA3.198e-5658.70ATP-dependent RNA helicase eIF4A OS=Kluyveromyces ... [more]
RH2_ARATH9.305e-5657.61DEAD-box ATP-dependent RNA helicase 2 OS=Arabidops... [more]
IF4A_YEAST1.215e-5561.41ATP-dependent RNA helicase eIF4A OS=Saccharomyces ... [more]
IF4A_YEAS71.215e-5561.41ATP-dependent RNA helicase eIF4A OS=Saccharomyces ... [more]
IF4A_CANGA6.031e-5559.78ATP-dependent RNA helicase eIF4A OS=Candida glabra... [more]
FAL1_YARLI7.877e-5556.52ATP-dependent RNA helicase FAL1 OS=Yarrowia lipoly... [more]
FAL1_DEBHA1.755e-5455.43ATP-dependent RNA helicase FAL1 OS=Debaryomyces ha... [more]

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Properties
Property NameValue
Genbank descriptionC32003B03EF DevOvary1 Citrus clementina cDNA clone C32003B03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC876300 ID=FC876300; Name=FC876300; organism=Citrus clementina; type=EST; length=558bp
AGGATCAGTCTGGAACTGGGAATCCAGCAACTTTCTGCTCTGGAATTTTG
CAGCAACTTGATTATGGTCTTGTCCAATGTCAGGCCCTGGTATTGGCACC
CACTAGGGAACTAGCTCAACAGATTGAGAAAGTAATGAGGGCACTTGGTG
ATTATCTTGGTGTTAAGGTTCATGCTTGTGTCGGTGGGACAAGTGTTCGC
GAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTTGTTGTTGGCACTCC
TGGTCGTGTGTTTGACATGCTGCGCCGACAGTCTTTACGCCCTGATTACA
TTAAGATGTTTGTACTGGATGAGGCTGATGAAATGCTTTCCCGTGGCTTT
AAGGATCAGATCTATGATATTTTCCAGCTGCTTCCAGCTAAAGTTCAAGT
GGGGGTTTTCTCTGCTACAATGCCTCCTGAGGCCTTGGAGATTACAAGGA
AGTTTATGAACAAGCCTGCGAGAATCTTGGTTAAACTTGATGAACTCACC
CTTGAGGGTATCAAGCAATTTTATCTCACTGTTGAGAACGAAGAATGGAA
GCTTGAAA
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