FC920219

Overview
NameFC920219
Unique NameFC920219
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length688
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA2_ORYSJ (Tubulin alpha-2 chain OS=Oryza sativa subsp. japonica GN=TUBA PE=2 SV=1)

HSP 1 Score: 348.206 bits (892), Expect = 3.704e-99
Identity = 171/193 (88.60%), Postives = 173/193 (89.64%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQM  DKTVGGGDDAFNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS
Sbjct:    1 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGDYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 193

HSP 2 Score: 35.4242 bits (80), Expect = 3.704e-99
Identity = 17/22 (77.27%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+VLLDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVLLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_DAUCA (Tubulin alpha chain OS=Daucus carota GN=TBA PE=2 SV=1)

HSP 1 Score: 347.051 bits (889), Expect = 3.704e-99
Identity = 167/193 (86.53%), Postives = 174/193 (90.16%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECIS+HIGQAGIQ+GNACWELYCLEHGIQP+GQM SDKTVGGGDDAFNTFFSETGAGKHVPRA+F+DLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFTVYPSPQ+STSVVEPYNSVLSTHS
Sbjct:    1 MRECISVHIGQAGIQIGNACWELYCLEHGIQPNGQMPSDKTVGGGDDAFNTFFSETGAGKHVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQISTSVVEPYNSVLSTHS 193

HSP 2 Score: 36.5798 bits (83), Expect = 3.704e-99
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDVSVLLDNE+IYDI
Sbjct:  192 HSL-LEHTDVSVLLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1_GOSHI (Tubulin alpha-1 chain OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 346.28 bits (887), Expect = 6.304e-99
Identity = 170/193 (88.08%), Postives = 172/193 (89.12%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQM SDKTVGGGDDAF TFFSETGAGKHVPRA+F+DLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF AV                 VDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS
Sbjct:    1 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFYTFFSETGAGKHVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFSAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 193

HSP 2 Score: 36.5798 bits (83), Expect = 6.304e-99
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDVSVLLDNE+IYDI
Sbjct:  192 HSL-LEHTDVSVLLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1_ELEIN (Tubulin alpha-1 chain OS=Eleusine indica GN=TUBA1 PE=2 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 2.383e-98
Identity = 169/193 (87.56%), Postives = 172/193 (89.12%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIHIGQAGIQVGNACWELYCLEHGIQ DGQM  DKT+GGGDDAFNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS
Sbjct:    1 MRECISIHIGQAGIQVGNACWELYCLEHGIQADGQMPGDKTIGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 193

HSP 2 Score: 35.4242 bits (80), Expect = 2.383e-98
Identity = 17/22 (77.27%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+VLLDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVLLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA2_MAIZE (Tubulin alpha-2 chain OS=Zea mays GN=TUBA2 PE=3 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 3.108e-98
Identity = 169/193 (87.56%), Postives = 172/193 (89.12%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIHIGQAGIQVGNACWELYCLEHGIQ DGQM  DKT+GGGDDAFNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS
Sbjct:    1 MRECISIHIGQAGIQVGNACWELYCLEHGIQADGQMPGDKTIGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 193

HSP 2 Score: 35.039 bits (79), Expect = 3.108e-98
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV++LLDNE+IYDI
Sbjct:  192 HSL-LEHTDVAILLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1_MAIZE (Tubulin alpha-1 chain OS=Zea mays GN=TUBA1 PE=3 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 3.108e-98
Identity = 169/193 (87.56%), Postives = 172/193 (89.12%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIHIGQAGIQVGNACWELYCLEHGIQ DGQM  DKT+GGGDDAFNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS
Sbjct:    1 MRECISIHIGQAGIQVGNACWELYCLEHGIQADGQMPGDKTIGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 193

HSP 2 Score: 35.039 bits (79), Expect = 3.108e-98
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV++LLDNE+IYDI
Sbjct:  192 HSL-LEHTDVAILLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_CHLVU (Tubulin alpha chain OS=Chlorella vulgaris GN=TUBA PE=3 SV=1)

HSP 1 Score: 344.739 bits (883), Expect = 4.055e-98
Identity = 169/193 (87.56%), Postives = 172/193 (89.12%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIHIGQAGIQVGNACWELYCLEHGIQPDGQM SDKT+GGGDDAFNTFFSETGAGKHVPR VFLDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFTVYPSPQVST+VVEPYNSVLSTHS
Sbjct:    1 MREVISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRCVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTAVVEPYNSVLSTHS 193

HSP 2 Score: 35.4242 bits (80), Expect = 4.055e-98
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDVSV+LDNE++YDI
Sbjct:  192 HSL-LEHTDVSVMLDNEAVYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_TETTH (Tubulin alpha chain OS=Tetrahymena thermophila PE=1 SV=1)

HSP 1 Score: 340.887 bits (873), Expect = 9.859e-97
Identity = 164/193 (84.97%), Postives = 172/193 (89.12%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIH+GQ GIQVGNACWEL+CLEHGIQPDGQM SDKT+GGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF++V                 VDYGKKSKLGFT+YPSPQVST+VVEPYNS+LSTHS
Sbjct:    1 MREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNSVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSILSTHS 193

HSP 2 Score: 34.6538 bits (78), Expect = 9.859e-97
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+V+LDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVMLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_TETPY (Tubulin alpha chain OS=Tetrahymena pyriformis PE=3 SV=1)

HSP 1 Score: 340.887 bits (873), Expect = 9.859e-97
Identity = 164/193 (84.97%), Postives = 172/193 (89.12%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIH+GQ GIQVGNACWEL+CLEHGIQPDGQM SDKT+GGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVF++V                 VDYGKKSKLGFT+YPSPQVST+VVEPYNS+LSTHS
Sbjct:    1 MREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNSVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSILSTHS 193

HSP 2 Score: 34.6538 bits (78), Expect = 9.859e-97
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+V+LDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVMLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA2_CHLRE (Tubulin alpha-2 chain OS=Chlamydomonas reinhardtii GN=TUBA2 PE=1 SV=1)

HSP 1 Score: 340.502 bits (872), Expect = 1.286e-96
Identity = 166/193 (86.01%), Postives = 171/193 (88.60%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIHIGQAGIQVGNACWELYCLEHGIQPDGQM SDKT+GGGDDAFNTFFSETGAGKHVPR +FLDLEPTV+DEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDL LDRIRKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFTVYPSPQVST+VVEPYNSVLSTHS
Sbjct:    1 MREVISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRCIFLDLEPTVVDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLALDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTAVVEPYNSVLSTHS 193

HSP 2 Score: 34.6538 bits (78), Expect = 1.286e-96
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+V+LDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVMLDNEAIYDI 212
The following BLAST results are available for this feature:
BLAST of FC920219 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 413
Match NameE-valueIdentityDescription
TBA2_ORYSJ3.704e-9988.60Tubulin alpha-2 chain OS=Oryza sativa subsp. japon... [more]
TBA_DAUCA3.704e-9986.53Tubulin alpha chain OS=Daucus carota GN=TBA PE=2 S... [more]
TBA1_GOSHI6.304e-9988.08Tubulin alpha-1 chain OS=Gossypium hirsutum PE=2 S... [more]
TBA1_ELEIN2.383e-9887.56Tubulin alpha-1 chain OS=Eleusine indica GN=TUBA1 ... [more]
TBA2_MAIZE3.108e-9887.56Tubulin alpha-2 chain OS=Zea mays GN=TUBA2 PE=3 SV... [more]
TBA1_MAIZE3.108e-9887.56Tubulin alpha-1 chain OS=Zea mays GN=TUBA1 PE=3 SV... [more]
TBA_CHLVU4.055e-9887.56Tubulin alpha chain OS=Chlorella vulgaris GN=TUBA ... [more]
TBA_TETTH9.859e-9784.97Tubulin alpha chain OS=Tetrahymena thermophila PE=... [more]
TBA_TETPY9.859e-9784.97Tubulin alpha chain OS=Tetrahymena pyriformis PE=3... [more]
TBA2_CHLRE1.286e-9686.01Tubulin alpha-2 chain OS=Chlamydomonas reinhardtii... [more]

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Properties
Property NameValue
Genbank descriptionC32005E05EF RVDevelop1 Citrus clementina cDNA clone C32005E05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920219 ID=FC920219; Name=FC920219; organism=Citrus clementina; type=EST; length=688bp
GACTCTTAATCTCGCAATTCCCGACTCAAATCTCTAACAAAATCACACAA
GAATGAGAGAATGCATCTCAATTCACATCGGCCAGGCCGGTATCCAGGTT
GGTAATGCCTGCTGGGAGCTCTATTGCCTCGAACATGGCATTCAGCCTGA
TGGGCAAATGTTAAGTGACAAAACAGTTGGAGGCGGAGATGATGCTTTCA
ACACATTCTTCAGTGAGACCGGCGCCGGAAAGCACGTTCCACGCGCCGTG
TTTTTGGACCTTGAACCAACTGTAATCGATGAAGTGAGGACTGGCGCCTA
CCGTCAGTTGTTTCACCCGGAGCAACTCATTAGCGGCAAAGAAGATGCTG
CTAACAACTTTGCCAGAGGCCACTATACAATTGGGAAAGAGATTGTGGAT
TTGTGCCTTGATAGGATCAGGAAGTTAGCTGATAACTGTACCGGTCTACA
AGGGTTCCTCGTGTTCCACGCGGTTGGTGGAGGAACAGGATCCGGGCTCG
GGTCTTTGCTCCTTGAGAGGCTTTCTGTTGATTACGGCAAGAAATCGAAA
CTGGGATTTACAGTTTACCCTTCACCTCAAGTTTCAACCTCAGTTGTTGA
GCCTTACAACAGCGTTTTGTCCACTCACTCTCTGTTAGACACACTGACGT
TTCTGTGTTGCTTGACAATGAAAGCATCTACGATATTT
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