FC920219

Overview
NameFC920219
Unique NameFC920219
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length688
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig3443 contig Ccv1_Contig3443:1..677. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_NEOCA (Tubulin alpha chain OS=Neospora caninum GN=TUBA PE=2 SV=1)

HSP 1 Score: 326.25 bits (835), Expect = 2.407e-92
Identity = 158/193 (81.87%), Postives = 167/193 (86.53%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIH+GQAGIQ+GNACWEL+CLEHGIQPDGQM SDKT+GGGDDAFNTFFSETGAGKHVPR VFLDLEPTV+DEVRTG YR LFHPEQLISGKEDAANNFARGHYTIGKEIVDL LDRIRKLADNCTGLQGFL+F+AV                 VDYGKKSKL F  +PSPQVST+VVEPYNSVLSTHS
Sbjct:    1 MREVISIHVGQAGIQIGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRCVFLDLEPTVVDEVRTGTYRHLFHPEQLISGKEDAANNFARGHYTIGKEIVDLSLDRIRKLADNCTGLQGFLMFNAVGGGTGSGLGCLLLERLSVDYGKKSKLNFCSWPSPQVSTAVVEPYNSVLSTHS 193

HSP 2 Score: 34.6538 bits (78), Expect = 2.407e-92
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+V+LDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVMLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_AVESA (Tubulin alpha chain OS=Avena sativa GN=TUBA PE=2 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 3.141e-92
Identity = 159/193 (82.38%), Postives = 167/193 (86.53%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIHIGQAGIQVGNACWELYCLEHGIQ DG M SD TVG   DAFNTFFSETGAGKHVPRA+F+DLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCL R+R+LADNCTGLQGFLVF+AV                 VDYGKKSKLGFT+YPSPQVST+VVEPYNSVLSTHS
Sbjct:    1 MREIISIHIGQAGIQVGNACWELYCLEHGIQQDGTMPSDTTVGVAHDAFNTFFSETGAGKHVPRAIFVDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLGRVRQLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSVLSTHS 193

HSP 2 Score: 35.4242 bits (80), Expect = 3.141e-92
Identity = 17/22 (77.27%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+VLLDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVLLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA5_MAIZE (Tubulin alpha-5 chain OS=Zea mays GN=TUBA5 PE=2 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 5.345e-92
Identity = 157/193 (81.35%), Postives = 168/193 (87.05%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIHIGQAGIQVGNACWELYCLEHGI+ DG M SD +VG   DAFNTFFSETG+GKHVPRA+F+DLEPTVIDEVRTG+YRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLDR+RKLADNCTGLQGFLVF+AV                 VDYGKKSKLGFT+YPSPQVST+VVEPYNSVLSTHS
Sbjct:    1 MREIISIHIGQAGIQVGNACWELYCLEHGIEHDGTMPSDSSVGVAHDAFNTFFSETGSGKHVPRAIFVDLEPTVIDEVRTGSYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLDRVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSVLSTHS 193

HSP 2 Score: 35.4242 bits (80), Expect = 5.345e-92
Identity = 17/22 (77.27%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+VLLDNE+IYDI
Sbjct:  192 HSL-LEHTDVAVLLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1C_BOVIN (Tubulin alpha-1C chain OS=Bos taurus GN=TUBA1C PE=1 SV=1)

HSP 1 Score: 329.331 bits (843), Expect = 5.848e-91
Identity = 153/193 (79.27%), Postives = 170/193 (88.08%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLISGKEDAANN+ARGHYTIGKEI+DL LDR+RKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIIDLVLDRVRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 5.848e-91
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_TORMA (Tubulin alpha chain OS=Torpedo marmorata PE=2 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 9.952e-91
Identity = 153/193 (79.27%), Postives = 170/193 (88.08%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEI+DL LDRIRKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 9.952e-91
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA3_DROME (Tubulin alpha-3 chain OS=Drosophila melanogaster GN=alphaTub84D PE=1 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 9.952e-91
Identity = 154/193 (79.79%), Postives = 169/193 (87.56%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKTVGGGDD+FNTFFSETGAGKHVPRAVF+DLEPTV+DEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEIVDL LDRIRKLAD CTGLQGFL+FH+                  VDYGKKSKL F VYP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 9.952e-91
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1B_RAT (Tubulin alpha-1B chain OS=Rattus norvegicus GN=Tuba1b PE=1 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 9.952e-91
Identity = 153/193 (79.27%), Postives = 170/193 (88.08%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEI+DL LDRIRKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 9.952e-91
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1B_PIG (Tubulin alpha-1B chain OS=Sus scrofa GN=TUBA1B PE=2 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 9.952e-91
Identity = 153/193 (79.27%), Postives = 170/193 (88.08%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEI+DL LDRIRKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 9.952e-91
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1B_PANTR (Tubulin alpha-1B chain OS=Pan troglodytes GN=TUBA1B PE=2 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 9.952e-91
Identity = 153/193 (79.27%), Postives = 170/193 (88.08%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEI+DL LDRIRKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 9.952e-91
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1B_MOUSE (Tubulin alpha-1B chain OS=Mus musculus GN=Tuba1b PE=1 SV=2)

HSP 1 Score: 328.561 bits (841), Expect = 9.952e-91
Identity = 153/193 (79.27%), Postives = 170/193 (88.08%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTVIDEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEI+DL LDRIRKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 9.952e-91
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
The following BLAST results are available for this feature:
BLAST of FC920219 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 413
Match NameE-valueIdentityDescription
TBA_NEOCA2.407e-9281.87Tubulin alpha chain OS=Neospora caninum GN=TUBA PE... [more]
TBA_AVESA3.141e-9282.38Tubulin alpha chain OS=Avena sativa GN=TUBA PE=2 S... [more]
TBA5_MAIZE5.345e-9281.35Tubulin alpha-5 chain OS=Zea mays GN=TUBA5 PE=2 SV... [more]
TBA1C_BOVIN5.848e-9179.27Tubulin alpha-1C chain OS=Bos taurus GN=TUBA1C PE=... [more]
TBA_TORMA9.952e-9179.27Tubulin alpha chain OS=Torpedo marmorata PE=2 SV=1[more]
TBA3_DROME9.952e-9179.79Tubulin alpha-3 chain OS=Drosophila melanogaster G... [more]
TBA1B_RAT9.952e-9179.27Tubulin alpha-1B chain OS=Rattus norvegicus GN=Tub... [more]
TBA1B_PIG9.952e-9179.27Tubulin alpha-1B chain OS=Sus scrofa GN=TUBA1B PE=... [more]
TBA1B_PANTR9.952e-9179.27Tubulin alpha-1B chain OS=Pan troglodytes GN=TUBA1... [more]
TBA1B_MOUSE9.952e-9179.27Tubulin alpha-1B chain OS=Mus musculus GN=Tuba1b P... [more]

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Properties
Property NameValue
Genbank descriptionC32005E05EF RVDevelop1 Citrus clementina cDNA clone C32005E05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920219 ID=FC920219; Name=FC920219; organism=Citrus clementina; type=EST; length=688bp
GACTCTTAATCTCGCAATTCCCGACTCAAATCTCTAACAAAATCACACAA
GAATGAGAGAATGCATCTCAATTCACATCGGCCAGGCCGGTATCCAGGTT
GGTAATGCCTGCTGGGAGCTCTATTGCCTCGAACATGGCATTCAGCCTGA
TGGGCAAATGTTAAGTGACAAAACAGTTGGAGGCGGAGATGATGCTTTCA
ACACATTCTTCAGTGAGACCGGCGCCGGAAAGCACGTTCCACGCGCCGTG
TTTTTGGACCTTGAACCAACTGTAATCGATGAAGTGAGGACTGGCGCCTA
CCGTCAGTTGTTTCACCCGGAGCAACTCATTAGCGGCAAAGAAGATGCTG
CTAACAACTTTGCCAGAGGCCACTATACAATTGGGAAAGAGATTGTGGAT
TTGTGCCTTGATAGGATCAGGAAGTTAGCTGATAACTGTACCGGTCTACA
AGGGTTCCTCGTGTTCCACGCGGTTGGTGGAGGAACAGGATCCGGGCTCG
GGTCTTTGCTCCTTGAGAGGCTTTCTGTTGATTACGGCAAGAAATCGAAA
CTGGGATTTACAGTTTACCCTTCACCTCAAGTTTCAACCTCAGTTGTTGA
GCCTTACAACAGCGTTTTGTCCACTCACTCTCTGTTAGACACACTGACGT
TTCTGTGTTGCTTGACAATGAAAGCATCTACGATATTT
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