FC920219

Overview
NameFC920219
Unique NameFC920219
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length688
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig3443 contig Ccv1_Contig3443:1..677. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1_PARLI (Tubulin alpha-1 chain OS=Paracentrotus lividus PE=2 SV=1)

HSP 1 Score: 324.709 bits (831), Expect = 1.420e-89
Identity = 148/193 (76.68%), Postives = 169/193 (87.56%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTV+DEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYT+GKE++D+ LDRIRKLAD CTGLQGFL+FH+                  VDYGKKSKL F VYP+PQ+ST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKELIDIVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFSSLLMERLSVDYGKKSKLEFAVYPAPQISTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 1.420e-89
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA1_APLCA (Tubulin alpha-1 chain OS=Aplysia californica PE=2 SV=1)

HSP 1 Score: 323.939 bits (829), Expect = 2.417e-89
Identity = 148/193 (76.68%), Postives = 170/193 (88.08%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEP+V+DEVR+G YRQLFHPEQLI+GKEDAANN+ARGHYT+GKE++DL LDRIRKLAD CTGLQGFL+FH+                  VDYGKKSKL F+VYP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPSVVDEVRSGTYRQLFHPEQLITGKEDAANNYARGHYTVGKELIDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSVYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 2.417e-89
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA2_DROME (Tubulin alpha-2 chain OS=Drosophila melanogaster GN=alphaTub85E PE=1 SV=1)

HSP 1 Score: 323.939 bits (829), Expect = 2.417e-89
Identity = 151/193 (78.24%), Postives = 169/193 (87.56%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECIS+HIGQAG+Q+GNACWELYCLEHGIQPDG M SDKTVGGGDD+F+TFFSETGAGKHVPRAVF+DLEPTV+DEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEIVD+ LDRIRKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISVHIGQAGVQIGNACWELYCLEHGIQPDGHMPSDKTVGGGDDSFSTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDVVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 2.417e-89
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA2_ELEIN (Tubulin alpha-2 chain OS=Eleusine indica GN=TUBA2 PE=2 SV=1)

HSP 1 Score: 315.464 bits (807), Expect = 3.153e-89
Identity = 153/193 (79.27%), Postives = 166/193 (86.01%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIHIGQAGIQVGN+CWELYCLEHGIQPDG M SD ++G   DAFNTFFSETGAGKHVPRA+F+DLEPTVIDEV+TG YRQLFHPEQLIS KEDAANNFARGHYT+G+EIVD CLDRIRKLADNCTGLQGFLVF+AV                 VDYG+KSKLGFT+YPSPQ+ST+VVEPYNSVLSTHS
Sbjct:    1 MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGLMPSDTSLGVARDAFNTFFSETGAGKHVPRALFVDLEPTVIDEVKTGPYRQLFHPEQLISYKEDAANNFARGHYTVGREIVDPCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGRKSKLGFTIYPSPQISTAVVEPYNSVLSTHS 193

HSP 2 Score: 35.039 bits (79), Expect = 3.153e-89
Identity = 17/22 (77.27%), Postives = 19/22 (86.36%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV VLLDNE+IYDI
Sbjct:  192 HSL-IEHTDVVVLLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_NOTVI (Tubulin alpha chain OS=Notophthalmus viridescens PE=2 SV=1)

HSP 1 Score: 322.783 bits (826), Expect = 5.365e-89
Identity = 149/193 (77.20%), Postives = 168/193 (87.05%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECIS+H+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRA+F+DLEP+VIDEVRTG YRQLFHPEQLISGKEDAANN+ARGHYTIGKEI+D  LDR+RKLAD CTGLQGFLVFH+                  VDYGKKSKL F +YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAIFVDLEPSVIDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIIDQVLDRMRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 5.365e-89
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_LEPDS (Tubulin alpha chain OS=Lepidoglyphus destructor PE=1 SV=2)

HSP 1 Score: 322.398 bits (825), Expect = 6.998e-89
Identity = 149/193 (77.20%), Postives = 168/193 (87.05%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECIS+H+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+G GDD+FNTFFSETG+GKHVPRAV++DLEPTV+DEVRTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEIVDL LDRIRKLAD CTGLQGFL+FH+                  VDYGKKSKL F VYP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGTGDDSFNTFFSETGSGKHVPRAVYVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 6.998e-89
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA3E_HUMAN (Tubulin alpha-3E chain OS=Homo sapiens GN=TUBA3E PE=1 SV=2)

HSP 1 Score: 322.398 bits (825), Expect = 6.998e-89
Identity = 150/193 (77.72%), Postives = 168/193 (87.05%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFFSETGAGKHVPRAVF+DLEPTV+DEVRTG YRQLFHPEQLI+GKEDAA+N+ARGHYTIGKEIVDL LDRIRKLAD CTGLQGFL+FH+                  VDY KKSKL F +YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAASNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYSKKSKLEFAIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 6.998e-89
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA3_HOMAM (Tubulin alpha-3 chain OS=Homarus americanus PE=2 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 9.129e-89
Identity = 149/193 (77.20%), Postives = 167/193 (86.53%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECISIH+GQAG Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+FNTFF ETGAGKHVPRAVF+DLEPTVIDE+RTG YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEIVD+ LDRIRKLADNC GLQGFL+FH+                  VDYGKKSKL F +YP+PQV+T+VVEPYNS+L+TH+
Sbjct:    1 MRECISIHVGQAGAQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFCETGAGKHVPRAVFVDLEPTVIDEIRTGVYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDIVLDRIRKLADNCAGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVATAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 9.129e-89
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_PLAYO (Tubulin alpha chain OS=Plasmodium yoelii yoelii GN=PY01155 PE=2 SV=2)

HSP 1 Score: 313.538 bits (802), Expect = 2.026e-88
Identity = 151/193 (78.24%), Postives = 162/193 (83.94%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRE ISIH+GQAGIQVGNACWEL+CLEHGIQPDGQM  D+     DDAFNTFFSETGAGKHVPR VF+DLEPTV+DEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKE++D+CLDRIRKLADNCTGLQGFL+F AV                 VDYGKKSKL F  +PSPQVST+VVEPYNSVLSTHS
Sbjct:    1 MREVISIHVGQAGIQVGNACWELFCLEHGIQPDGQMPPDQAGRANDDAFNTFFSETGAGKHVPRCVFVDLEPTVVDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEVIDVCLDRIRKLADNCTGLQGFLMFSAVGGGTGSGFGCLMLERLSVDYGKKSKLNFCCWPSPQVSTAVVEPYNSVLSTHS 193

HSP 2 Score: 34.2686 bits (77), Expect = 2.026e-88
Identity = 15/22 (68.18%), Postives = 20/22 (90.91%), Query Frame = 1 
Query:  622 HSLSVRHTDVSVLLDNESIYDI 687
            HSL + HTDV+++LDNE+IYDI
Sbjct:  192 HSL-LEHTDVAIMLDNEAIYDI 212
[Close]
BLAST of FC920219 vs. ExPASy Swiss-Prot
Match: TBA_XENTR (Tubulin alpha chain OS=Xenopus tropicalis GN=tuba PE=2 SV=1)

HSP 1 Score: 320.472 bits (820), Expect = 2.644e-88
Identity = 149/193 (77.20%), Postives = 167/193 (86.53%), Query Frame = 2 
Query:   53 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMLSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHS 631
            MRECIS+H+GQAG+Q+GNACWELYCLEHGIQPDGQM SDKT+GGGDD+F TFFSETGAGKHVPRAVF+DLEPTVIDEVR G YRQLFHPEQLI+GKEDAANN+ARGHYTIGKEI+D  LDRIRKLAD CTGLQGFLVFH+                  VDYGKKSKL F++YP+PQVST+VVEPYNS+L+TH+
Sbjct:    1 MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFSETGAGKHVPRAVFVDLEPTVIDEVRAGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHT 193

HSP 2 Score: 26.9498 bits (58), Expect = 2.644e-88
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  631 SVRHTDVSVLLDNESIYDI 687
            ++ H+D + ++DNE+IYDI
Sbjct:  194 TLEHSDCAFMVDNEAIYDI 212
The following BLAST results are available for this feature:
BLAST of FC920219 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 413
Match NameE-valueIdentityDescription
TBA1_PARLI1.420e-8976.68Tubulin alpha-1 chain OS=Paracentrotus lividus PE=... [more]
TBA1_APLCA2.417e-8976.68Tubulin alpha-1 chain OS=Aplysia californica PE=2 ... [more]
TBA2_DROME2.417e-8978.24Tubulin alpha-2 chain OS=Drosophila melanogaster G... [more]
TBA2_ELEIN3.153e-8979.27Tubulin alpha-2 chain OS=Eleusine indica GN=TUBA2 ... [more]
TBA_NOTVI5.365e-8977.20Tubulin alpha chain OS=Notophthalmus viridescens P... [more]
TBA_LEPDS6.998e-8977.20Tubulin alpha chain OS=Lepidoglyphus destructor PE... [more]
TBA3E_HUMAN6.998e-8977.72Tubulin alpha-3E chain OS=Homo sapiens GN=TUBA3E P... [more]
TBA3_HOMAM9.129e-8977.20Tubulin alpha-3 chain OS=Homarus americanus PE=2 S... [more]
TBA_PLAYO2.026e-8878.24Tubulin alpha chain OS=Plasmodium yoelii yoelii GN... [more]
TBA_XENTR2.644e-8877.20Tubulin alpha chain OS=Xenopus tropicalis GN=tuba ... [more]

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Properties
Property NameValue
Genbank descriptionC32005E05EF RVDevelop1 Citrus clementina cDNA clone C32005E05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920219 ID=FC920219; Name=FC920219; organism=Citrus clementina; type=EST; length=688bp
GACTCTTAATCTCGCAATTCCCGACTCAAATCTCTAACAAAATCACACAA
GAATGAGAGAATGCATCTCAATTCACATCGGCCAGGCCGGTATCCAGGTT
GGTAATGCCTGCTGGGAGCTCTATTGCCTCGAACATGGCATTCAGCCTGA
TGGGCAAATGTTAAGTGACAAAACAGTTGGAGGCGGAGATGATGCTTTCA
ACACATTCTTCAGTGAGACCGGCGCCGGAAAGCACGTTCCACGCGCCGTG
TTTTTGGACCTTGAACCAACTGTAATCGATGAAGTGAGGACTGGCGCCTA
CCGTCAGTTGTTTCACCCGGAGCAACTCATTAGCGGCAAAGAAGATGCTG
CTAACAACTTTGCCAGAGGCCACTATACAATTGGGAAAGAGATTGTGGAT
TTGTGCCTTGATAGGATCAGGAAGTTAGCTGATAACTGTACCGGTCTACA
AGGGTTCCTCGTGTTCCACGCGGTTGGTGGAGGAACAGGATCCGGGCTCG
GGTCTTTGCTCCTTGAGAGGCTTTCTGTTGATTACGGCAAGAAATCGAAA
CTGGGATTTACAGTTTACCCTTCACCTCAAGTTTCAACCTCAGTTGTTGA
GCCTTACAACAGCGTTTTGTCCACTCACTCTCTGTTAGACACACTGACGT
TTCTGTGTTGCTTGACAATGAAAGCATCTACGATATTT
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