FC920847

Overview
NameFC920847
Unique NameFC920847
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length698
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig10260 contig Ccv1_Contig10260:1..696. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHSY_PINSY (Chalcone synthase OS=Pinus sylvestris GN=CHS PE=3 SV=1)

HSP 1 Score: 318.546 bits (815), Expect = 8.538e-99
Identity = 152/180 (84.44%), Postives = 167/180 (92.78%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M  ++  R AQRADGPAT+LAIGTATP ++V+Q+ YPDYYF+IT SEHMTELKEKF+RMCDKS IKKRYMYLTEEILKENP +C YMAPSLDARQD+VVVEVP+LGKEAA KAIKEWGQPKSKITH+IFCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTVLR+AKDLAEN
Sbjct:    6 MKDLEAFRKAQRADGPATILAIGTATPPNAVDQSSYPDYYFKITNSEHMTELKEKFRRMCDKSAIKKRYMYLTEEILKENPKVCEYMAPSLDARQDMVVVEVPRLGKEAAAKAIKEWGQPKSKITHVIFCTTSGVDMPGADYQLTKLLGLRPSVKRVMMYQQGCFAGGTVLRVAKDLAEN 185

HSP 2 Score: 63.5438 bits (153), Expect = 8.538e-99
Identity = 29/33 (87.88%), Postives = 33/33 (100.00%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            ++GARVLVVCSEITAVTFRGP+DTHLDS+VGQA
Sbjct:  186 NRGARVLVVCSEITAVTFRGPSDTHLDSMVGQA 218
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS9_MEDSA (Chalcone synthase 9 OS=Medicago sativa GN=CHS9 PE=2 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 8.539e-99
Identity = 151/180 (83.89%), Postives = 167/180 (92.78%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M +V EIR AQRA+GPAT++AIGTA PA+ V Q+ YPD+YF+IT SEH  ELKEKF+RMCDKSMIK+RYMYLTEEILKENP++C YMAPSLDARQD+VVVEVP+LGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRP VKR+MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVSVSEIRQAQRAEGPATIMAIGTANPANCVEQSTYPDFYFKITNSEHKVELKEKFQRMCDKSMIKRRYMYLTEEILKENPSVCEYMAPSLDARQDMVVVEVPRLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 8.539e-99
Identity = 30/33 (90.91%), Postives = 33/33 (100.00%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSE+TAVTFRGP+DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEVTAVTFRGPSDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS8_MEDSA (Chalcone synthase 8 OS=Medicago sativa GN=CHS8 PE=2 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 8.539e-99
Identity = 152/180 (84.44%), Postives = 167/180 (92.78%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M +V EIR AQRA+GPAT+LAIGTA PA+ V Q+ YPD+YF+IT SEH TELKEKF+RMCDKSMIK+RYMYLTEEILKENP++C YMAPSLDARQD+VVVEVP+LGKEAA KAIKEWGQPKSKITHLI CTTSGVDMPGADYQLTKL+GLRP VKR+MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVSVSEIRTAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRMCDKSMIKRRYMYLTEEILKENPSVCEYMAPSLDARQDMVVVEVPRLGKEAAVKAIKEWGQPKSKITHLIVCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 8.539e-99
Identity = 30/33 (90.91%), Postives = 33/33 (100.00%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSE+TAVTFRGP+DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEVTAVTFRGPSDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS4_MEDSA (Chalcone synthase 4 OS=Medicago sativa GN=CHS4 PE=2 SV=2)

HSP 1 Score: 317.005 bits (811), Expect = 8.539e-99
Identity = 152/180 (84.44%), Postives = 167/180 (92.78%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M +V EIR AQRA+GPAT+LAIGTA PA+ V Q+ YPD+YF+IT SEH TELKEKF+RMCDKSMIK+RYMYLTEEILKENP++C YMAPSLDARQD+VVVEVP+LGKEAA KAIKEWGQPKSKITHLI CTTSGVDMPGADYQLTKL+GLRP VKR+MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVSVSEIRKAQRAEGPATILAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRMCDKSMIKRRYMYLTEEILKENPSVCEYMAPSLDARQDMVVVEVPRLGKEAAVKAIKEWGQPKSKITHLIVCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 8.539e-99
Identity = 30/33 (90.91%), Postives = 33/33 (100.00%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSE+TAVTFRGP+DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEVTAVTFRGPSDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHSY_ORYSJ (Chalcone synthase OS=Oryza sativa subsp. japonica GN=CHS PE=2 SV=1)

HSP 1 Score: 322.783 bits (826), Expect = 1.114e-98
Identity = 154/178 (86.52%), Postives = 168/178 (94.38%), Query Frame = 1 
Query:   67 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            TV+E+R AQRA+GPATVLAIGTATPA+ V QADYPDYYFRITKSEHM ELKEKFKRMCDKS I+KRYM+LTEEIL+ENPNMCAYMAPSLDARQDIVVVEVPKLGK AA KAIKEWGQP+S+ITHL+FCTTSGVDMPGADYQL K++GLRP+V R MMYQQGCFAGGTVLR+AKDLAEN
Sbjct:    6 TVEEVRRAQRAEGPATVLAIGTATPANCVYQADYPDYYFRITKSEHMVELKEKFKRMCDKSQIRKRYMHLTEEILQENPNMCAYMAPSLDARQDIVVVEVPKLGKAAAQKAIKEWGQPRSRITHLVFCTTSGVDMPGADYQLAKMLGLRPNVNRLMMYQQGCFAGGTVLRVAKDLAEN 183

HSP 2 Score: 58.9214 bits (141), Expect = 1.114e-98
Identity = 26/33 (78.79%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            ++GARVL VCSEITAVTFRGP+++HLDS+VGQA
Sbjct:  184 NRGARVLAVCSEITAVTFRGPSESHLDSMVGQA 216
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHSY_ORYSI (Chalcone synthase OS=Oryza sativa subsp. indica GN=CHS PE=2 SV=1)

HSP 1 Score: 322.783 bits (826), Expect = 1.114e-98
Identity = 154/178 (86.52%), Postives = 168/178 (94.38%), Query Frame = 1 
Query:   67 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            TV+E+R AQRA+GPATVLAIGTATPA+ V QADYPDYYFRITKSEHM ELKEKFKRMCDKS I+KRYM+LTEEIL+ENPNMCAYMAPSLDARQDIVVVEVPKLGK AA KAIKEWGQP+S+ITHL+FCTTSGVDMPGADYQL K++GLRP+V R MMYQQGCFAGGTVLR+AKDLAEN
Sbjct:    6 TVEEVRRAQRAEGPATVLAIGTATPANCVYQADYPDYYFRITKSEHMVELKEKFKRMCDKSQIRKRYMHLTEEILQENPNMCAYMAPSLDARQDIVVVEVPKLGKAAAQKAIKEWGQPRSRITHLVFCTTSGVDMPGADYQLAKMLGLRPNVSRLMMYQQGCFAGGTVLRVAKDLAEN 183

HSP 2 Score: 58.9214 bits (141), Expect = 1.114e-98
Identity = 26/33 (78.79%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            ++GARVL VCSEITAVTFRGP+++HLDS+VGQA
Sbjct:  184 NRGARVLAVCSEITAVTFRGPSESHLDSMVGQA 216
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS2_IPOBA (Chalcone synthase LF2 OS=Ipomoea batatas GN=CHS-LF2 PE=2 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 1.453e-98
Identity = 153/180 (85.00%), Postives = 165/180 (91.67%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M TV+E+R AQRA+GPAT+LAIGT TPA+ VNQ+ YPDYYFRIT SEH TELKEKF+RMCDKSMI KRYM+LTEEILKENP+ C YMAPSLDARQDI VVEVPKLGKEAA  AIKEWGQPKSKITH++FCTTSGVDMPGADYQLTKL+GL PSVKR MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVTVEEVRKAQRAEGPATILAIGTVTPANCVNQSTYPDYYFRITNSEHKTELKEKFQRMCDKSMITKRYMHLTEEILKENPSFCEYMAPSLDARQDIAVVEVPKLGKEAAQSAIKEWGQPKSKITHVVFCTTSGVDMPGADYQLTKLLGLSPSVKRLMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 62.003 bits (149), Expect = 1.453e-98
Identity = 28/33 (84.85%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVL+VCSEIT VTFRGP++THLDSLVGQA
Sbjct:  181 NKGARVLIVCSEITVVTFRGPSETHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS8_BROFI (Chalcone synthase 8 OS=Bromheadia finlaysoniana GN=CHS8 PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 1.896e-98
Identity = 152/177 (85.88%), Postives = 168/177 (94.92%), Query Frame = 1 
Query:   70 VQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            ++EIR AQRA+GPA VLAIGT+TP +++ QADYPDYYFRITKSEH+TELKEKFKRMCDKSMIKKRYMYLTEEILKENPN+CA+MAPSLDARQDIVV EVPKL KEAA +AIKEWG PKS+ITHLIFCTTSG+DMPGADYQLT+L+GLRPSV RFM+YQQGCFAGGTVLRLAKDLAEN
Sbjct:    5 MEEIRQAQRAEGPAAVLAIGTSTPPNALYQADYPDYYFRITKSEHLTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNICAFMAPSLDARQDIVVTEVPKLAKEAAARAIKEWGHPKSRITHLIFCTTSGIDMPGADYQLTRLLGLRPSVNRFMLYQQGCFAGGTVLRLAKDLAEN 181

HSP 2 Score: 60.077 bits (144), Expect = 1.896e-98
Identity = 28/31 (90.32%), Postives = 31/31 (100.00%), Query Frame = 3 
Query:  606 GARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            GARVLVVCSEITAVTFRGP+++HLDSLVGQA
Sbjct:  184 GARVLVVCSEITAVTFRGPSESHLDSLVGQA 214
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS4_BROFI (Chalcone synthase 4 OS=Bromheadia finlaysoniana GN=CHS4 PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 1.896e-98
Identity = 152/177 (85.88%), Postives = 168/177 (94.92%), Query Frame = 1 
Query:   70 VQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            ++EIR AQRA+GPA VLAIGT+TP +++ QADYPDYYFRITKSEH+TELKEKFKRMCDKSMIKKRYMYLTEEILKENPN+CA+MAPSLDARQDIVV EVPKL KEAA +AIKEWG PKS+ITHLIFCTTSG+DMPGADYQLT+L+GLRPSV RFM+YQQGCFAGGTVLRLAKDLAEN
Sbjct:    5 MEEIRQAQRAEGPAAVLAIGTSTPPNALYQADYPDYYFRITKSEHLTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNICAFMAPSLDARQDIVVTEVPKLAKEAAVRAIKEWGHPKSRITHLIFCTTSGIDMPGADYQLTRLLGLRPSVNRFMLYQQGCFAGGTVLRLAKDLAEN 181

HSP 2 Score: 60.077 bits (144), Expect = 1.896e-98
Identity = 28/31 (90.32%), Postives = 31/31 (100.00%), Query Frame = 3 
Query:  606 GARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            GARVLVVCSEITAVTFRGP+++HLDSLVGQA
Sbjct:  184 GARVLVVCSEITAVTFRGPSESHLDSLVGQA 214
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS3_BROFI (Chalcone synthase 3 OS=Bromheadia finlaysoniana GN=CHS3 PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 1.896e-98
Identity = 152/177 (85.88%), Postives = 168/177 (94.92%), Query Frame = 1 
Query:   70 VQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            ++EIR AQRA+GPA VLAIGT+TP +++ QADYPDYYFRITKSEH+TELKEKFKRMCDKSMIKKRYMYLTEEILKENPN+CA+MAPSLDARQDIVV EVPKL KEAA +AIKEWG PKS+ITHLIFCTTSG+DMPGADYQLT+L+GLRPSV RFM+YQQGCFAGGTVLRLAKDLAEN
Sbjct:    5 MEEIRQAQRAEGPAAVLAIGTSTPPNALYQADYPDYYFRITKSEHLTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNICAFMAPSLDARQDIVVTEVPKLAKEAAVRAIKEWGHPKSRITHLIFCTTSGIDMPGADYQLTRLLGLRPSVNRFMLYQQGCFAGGTVLRLAKDLAEN 181

HSP 2 Score: 60.077 bits (144), Expect = 1.896e-98
Identity = 28/31 (90.32%), Postives = 31/31 (100.00%), Query Frame = 3 
Query:  606 GARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            GARVLVVCSEITAVTFRGP+++HLDSLVGQA
Sbjct:  184 GARVLVVCSEITAVTFRGPSESHLDSLVGQA 214
The following BLAST results are available for this feature:
BLAST of FC920847 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 145
Match NameE-valueIdentityDescription
CHSY_PINSY8.538e-9984.44Chalcone synthase OS=Pinus sylvestris GN=CHS PE=3 ... [more]
CHS9_MEDSA8.539e-9983.89Chalcone synthase 9 OS=Medicago sativa GN=CHS9 PE=... [more]
CHS8_MEDSA8.539e-9984.44Chalcone synthase 8 OS=Medicago sativa GN=CHS8 PE=... [more]
CHS4_MEDSA8.539e-9984.44Chalcone synthase 4 OS=Medicago sativa GN=CHS4 PE=... [more]
CHSY_ORYSJ1.114e-9886.52Chalcone synthase OS=Oryza sativa subsp. japonica ... [more]
CHSY_ORYSI1.114e-9886.52Chalcone synthase OS=Oryza sativa subsp. indica GN... [more]
CHS2_IPOBA1.453e-9885.00Chalcone synthase LF2 OS=Ipomoea batatas GN=CHS-LF... [more]
CHS8_BROFI1.896e-9885.88Chalcone synthase 8 OS=Bromheadia finlaysoniana GN... [more]
CHS4_BROFI1.896e-9885.88Chalcone synthase 4 OS=Bromheadia finlaysoniana GN... [more]
CHS3_BROFI1.896e-9885.88Chalcone synthase 3 OS=Bromheadia finlaysoniana GN... [more]

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Properties
Property NameValue
Genbank descriptionC32013G05EF RVDevelop1 Citrus clementina cDNA clone C32013G05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920847 ID=FC920847; Name=FC920847; organism=Citrus clementina; type=EST; length=698bp
TCGCCGGGGGAACTCACCAAGCACGAGCCACCAACATATTCATTAAGGGT
TCAACTTGAAATGGCAACCGTTCAAGAGATCAGAAACGCTCAGCGTGCCG
ACGGCCCGGCCACCGTCCTCGCCATCGGTACGGCCACGCCTGCCCACAGT
GTCAACCAGGCTGATTATCCCGACTATTACTTCAGGATCACAAAGAGCGA
GCATATGACGGAGCTTAAAGAGAAGTTCAAGCGCATGTGTGACAAGTCGA
TGATTAAGAAGCGTTACATGTACTTAACGGAAGAGATTTTGAAGGAAAAC
CCCAACATGTGCGCTTACATGGCTCCATCACTCGACGCACGTCAAGACAT
TGTGGTGGTCGAAGTGCCGAAGCTCGGGAAAGAAGCTGCTACAAAGGCCA
TCAAAGAATGGGGCCAGCCCAAGTCTAAGATCACCCACCTCATCTTCTGC
ACCACCTCCGGCGTCGACATGCCCGGCGCCGACTACCAGCTCACCAAACT
GATCGGCCTGCGCCCCTCCGTCAAGCGCTTCATGATGTACCAACAAGGAT
GTTTCGCCGGTGGCACTGTTCTTCGCCTCGCTAAAGACTTGGCTGAGAAC
ACAAGGGCGCTCGCGTTCTTGTTGTCTGTTCGGAGATCACGGCGGTCACT
TTCCGCGGCCCTGCCGATACTCATCTTGATTCTCTTGTGGGNCAGGCT
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