FC923255

Overview
NameFC923255
Unique NameFC923255
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length745
Libraries
Library NameType
StrClemenNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: PAPS2_MOUSE (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Mus musculus GN=Papss2 PE=1 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 3.043e-15
Identity = 22/46 (47.83%), Postives = 30/46 (65.22%), Query Frame = 3 
Query:   78 KDRDACRSMLPEGD--FIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            KDR+  R +       F E+F+D PL +CE+RD KGLYK AR G++
Sbjct:  128 KDRENARKIHESAGLPFFEIFVDAPLNICESRDVKGLYKRARAGEI 173

HSP 2 Score: 41.5874 bits (96), Expect = 3.043e-15
Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGLN++L F A DR ENIRRI ++
Sbjct:   80 DNVRHGLNKNLGFSAGDREENIRRIAEV 107

HSP 3 Score: 32.7278 bits (73), Expect = 3.043e-15
Identity = 19/47 (40.43%), Postives = 25/47 (53.19%), Query Frame = 2 
Query:  203 EGFTGIDDPYEPPLNCEIVLKQTGDDCPSPADMVETVVSYLDDNGFL 343
            +GFTGID  YE P   E VLK    +  S +D V+ VV  L +   +
Sbjct:  174 KGFTGIDSDYEKPETPECVLK---TNLSSVSDCVQQVVELLQEQNIV 217
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: CYSC_ERWT9 (Adenylyl-sulfate kinase OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=cysC PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 7.192e-15
Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = 3 
Query:   81 DRDACRSMLPEGDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            +R   R ML EG FIEVF+D PL +CEARDPKGLYK AR G++
Sbjct:  114 ERQMVRDMLGEGRFIEVFVDTPLAICEARDPKGLYKKARAGEL 156

HSP 2 Score: 39.6614 bits (91), Expect = 7.192e-15
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGL RDL F  +DR ENIRR+ ++
Sbjct:   65 DNVRHGLCRDLGFSDDDRKENIRRVGEV 92
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: NODQ_RHIME (Bifunctional enzyme nodQ OS=Rhizobium meliloti GN=nodQ PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.524e-14
Identity = 22/43 (51.16%), Postives = 30/43 (69.77%), Query Frame = 3 
Query:   81 DRDACRSMLPEGDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            +R   R ++ EG+FIE+F+D PL  C  RDPKGLY+ A  GK+
Sbjct:  546 ERRMARELMEEGEFIEIFVDTPLDECARRDPKGLYEKALAGKI 588

HSP 2 Score: 46.9802 bits (110), Expect = 1.524e-14
Identity = 20/28 (71.43%), Postives = 23/28 (82.14%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGLNRDL F  EDRVENIRR+ ++
Sbjct:  497 DNVRHGLNRDLGFTEEDRVENIRRVAEV 524
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: CYSC_CYAP7 (Adenylyl-sulfate kinase OS=Cyanothece sp. (strain PCC 7424) GN=cysC PE=3 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 2.571e-14
Identity = 21/32 (65.62%), Postives = 26/32 (81.25%), Query Frame = 3 
Query:  114 GDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            GDF+EVF++ PL VCE RD KGLYK AR G++
Sbjct:  100 GDFVEVFVNAPLAVCEQRDVKGLYKRARAGEI 131

HSP 2 Score: 41.2022 bits (95), Expect = 2.571e-14
Identity = 21/48 (43.75%), Postives = 28/48 (58.33%), Query Frame = 2 
Query:  203 EGFTGIDDPYEPPLNCEIVLKQTGDDCPSPADMVETVVSYLDDNGFLR 346
            + FTGIDDPYEPPLN E+   +   D     + V  ++  LD  G+LR
Sbjct:  132 KSFTGIDDPYEPPLNPEV---ECRTDLEELDESVNKILHKLDQLGYLR 176

HSP 3 Score: 28.1054 bits (61), Expect = 2.571e-14
Identity = 14/25 (56.00%), Postives = 15/25 (60.00%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRI 75
            D  R  L + L F  EDR ENIRRI
Sbjct:   42 DVVRTNLTKGLGFSKEDRDENIRRI 66
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: PAPS2_HUMAN (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 47.7506 bits (112), Expect = 3.089e-14
Identity = 22/46 (47.83%), Postives = 30/46 (65.22%), Query Frame = 3 
Query:   78 KDRDACRSMLPEGD--FIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            KDR+  R +       F E+F+D PL +CE+RD KGLYK AR G++
Sbjct:  127 KDRENARKIHESAGLPFFEIFVDAPLNICESRDVKGLYKRARAGEI 172

HSP 2 Score: 40.817 bits (94), Expect = 3.089e-14
Identity = 18/28 (64.29%), Postives = 21/28 (75.00%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGLNR+L F   DR ENIRRI ++
Sbjct:   79 DNVRHGLNRNLGFSPGDREENIRRIAEV 106

HSP 3 Score: 30.0314 bits (66), Expect = 3.089e-14
Identity = 18/47 (38.30%), Postives = 24/47 (51.06%), Query Frame = 2 
Query:  203 EGFTGIDDPYEPPLNCEIVLKQTGDDCPSPADMVETVVSYLDDNGFL 343
            +GFTGID  YE P   E VLK    +  + +D V  VV  L +   +
Sbjct:  173 KGFTGIDSDYEKPETPERVLK---TNLSTVSDCVHQVVELLQEQNIV 216
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: CYSC_CLOAB (Adenylyl-sulfate kinase OS=Clostridium acetobutylicum GN=cysC PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 3.462e-14
Identity = 23/44 (52.27%), Postives = 35/44 (79.55%), Query Frame = 3 
Query:   78 KDRDACRSMLPEGDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            +DR+  R +L + DF+EV++D P++VCE RDPKG+YK AR G++
Sbjct:  114 EDRNNARKLLGK-DFVEVYIDCPIEVCEKRDPKGIYKKARNGEI 156

HSP 2 Score: 44.2838 bits (103), Expect = 3.462e-14
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN R+GLN DL FK+EDR ENIRR+ ++
Sbjct:   66 DNLRYGLNSDLGFKSEDRTENIRRVSEV 93
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: CYSC_VIBVY (Adenylyl-sulfate kinase OS=Vibrio vulnificus (strain YJ016) GN=cysC PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.493e-14
Identity = 26/43 (60.47%), Postives = 34/43 (79.07%), Query Frame = 3 
Query:   81 DRDACRSMLPEGDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            +R   R +LPEG+FIEVF++  L+VCE RDPKGLYK AR G++
Sbjct:  118 ERQLVRDLLPEGEFIEVFVNTSLEVCEQRDPKGLYKKARAGEI 160

HSP 2 Score: 37.7354 bits (86), Expect = 4.493e-14
Identity = 17/28 (60.71%), Postives = 20/28 (71.43%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGL  DL F  +DR ENIRRI ++
Sbjct:   69 DNVRHGLCSDLGFSTQDRRENIRRIGEL 96
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: CYSC_VIBVU (Adenylyl-sulfate kinase OS=Vibrio vulnificus GN=cysC PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.493e-14
Identity = 26/43 (60.47%), Postives = 34/43 (79.07%), Query Frame = 3 
Query:   81 DRDACRSMLPEGDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            +R   R +LPEG+FIEVF++  L+VCE RDPKGLYK AR G++
Sbjct:  118 ERQLVRDLLPEGEFIEVFVNTSLEVCEQRDPKGLYKKARAGEI 160

HSP 2 Score: 37.7354 bits (86), Expect = 4.493e-14
Identity = 17/28 (60.71%), Postives = 20/28 (71.43%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGL  DL F  +DR ENIRRI ++
Sbjct:   69 DNVRHGLCSDLGFSTQDRRENIRRIGEL 96
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: CYSC_VIBPA (Adenylyl-sulfate kinase OS=Vibrio parahaemolyticus GN=cysC PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.496e-14
Identity = 26/43 (60.47%), Postives = 34/43 (79.07%), Query Frame = 3 
Query:   81 DRDACRSMLPEGDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            +R   R +LPEG+FIEVF++  L+VCE RDPKGLYK AR G++
Sbjct:  118 ERQLVRDLLPEGEFIEVFVNASLEVCEGRDPKGLYKKARAGEI 160

HSP 2 Score: 37.3502 bits (85), Expect = 4.496e-14
Identity = 17/28 (60.71%), Postives = 20/28 (71.43%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGL  DL F  +DR ENIRRI ++
Sbjct:   69 DNVRHGLCSDLGFSEQDRRENIRRIGEL 96
[Close]
BLAST of FC923255 vs. ExPASy Swiss-Prot
Match: CYSC1_BACHD (Probable adenylyl-sulfate kinase OS=Bacillus halodurans GN=BH1489 PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 4.499e-14
Identity = 25/44 (56.82%), Postives = 33/44 (75.00%), Query Frame = 3 
Query:   78 KDRDACRSMLPEGDFIEVFMDVPLQVCEARDPKGLYKLAREGKV 209
            +DRD  R +L +G+FIEV++  PL+ CE RDPKGLYK AR G +
Sbjct:  114 EDRDNVRGILDDGEFIEVYVRCPLETCEKRDPKGLYKKARSGDI 157

HSP 2 Score: 40.0466 bits (92), Expect = 4.499e-14
Identity = 18/28 (64.29%), Postives = 20/28 (71.43%), Query Frame = 1 
Query:    1 DNCRHGLNRDLSFKAEDRVENIRRIEKI 84
            DN RHGLN  L F  EDR ENIRRI ++
Sbjct:   66 DNIRHGLNAGLGFSEEDRKENIRRIGEV 93
The following BLAST results are available for this feature:
BLAST of FC923255 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
PAPS2_MOUSE3.043e-1547.83Bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
CYSC_ERWT97.192e-1565.12Adenylyl-sulfate kinase OS=Erwinia tasmaniensis (s... [more]
NODQ_RHIME1.524e-1451.16Bifunctional enzyme nodQ OS=Rhizobium meliloti GN=... [more]
CYSC_CYAP72.571e-1465.63Adenylyl-sulfate kinase OS=Cyanothece sp. (strain ... [more]
PAPS2_HUMAN3.089e-1447.83Bifunctional 3'-phosphoadenosine 5'-phosphosulfate... [more]
CYSC_CLOAB3.462e-1452.27Adenylyl-sulfate kinase OS=Clostridium acetobutyli... [more]
CYSC_VIBVY4.493e-1460.47Adenylyl-sulfate kinase OS=Vibrio vulnificus (stra... [more]
CYSC_VIBVU4.493e-1460.47Adenylyl-sulfate kinase OS=Vibrio vulnificus GN=cy... [more]
CYSC_VIBPA4.496e-1460.47Adenylyl-sulfate kinase OS=Vibrio parahaemolyticus... [more]
CYSC1_BACHD4.499e-1456.82Probable adenylyl-sulfate kinase OS=Bacillus halod... [more]

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Properties
Property NameValue
Genbank descriptionC31702G02EF StrClemenN Citrus clementina cDNA clone C31702G02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC923255 ID=FC923255; Name=FC923255; organism=Citrus clementina; type=EST; length=745bp
GACAACTGCCGGCATGGACTGAACCGTGATCTAAGTTTTAAGGCAGAAGA
TCGTGTGGAAAACATACGCAGGATTGAAAAGATCGAGATGCCTGCCGTTC
AATGTTGCCAGAAGGAGATTTCATTGAGGTGTTCATGGATGTGCCACTTC
AAGTGTGTGAGGCAAGGGACCCAAAGGGCCTTTACAAGCTTGCACGAGAA
GGGAAGGTTTTACTGGGATTGATGATCCATATGAACCACCATTAAACTGT
GAGATAGTACTAAAACAGACGGGAGATGACTGTCCTTCACCGGCTGATAT
GGTTGAAACTGTGGTATCTTACCTGGATGATAATGGCTTCCTGCGGGCCT
AATTTGAAGAGAAAGGTCTCAGCTCTTGGTTTCCATTGCCTTTGGTCACC
TGCAATACTTTGAGGTATCGACAAAAATCATGGTACGATCCAGTAAGGAA
ACTGATTGTTTCCTGCATCTGTAGCGACAATAAATGTCTGGCACTTTAAT
TACTCGATCGAGTAGTTGACGTCAGTGCCAAGCAAGTTGCATAGCGTTTC
GCGTCTTTAATTCAGGTTTGTTTTGGAATAAGTTATATGTTCATAGGGTT
GGGGATCGATGTAAATCAGTGTCATGCAGCATTAGAATAAAAGTTGTCTC
AGGTTTGCAAATTTCGATTGTACACGTACCCAACCCCGGCATCTTTGTAT
TACCATATTTGTGATAAGGAGCCAAGCTCTTACAGGCGTCTCATT
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