FC930319

Overview
NameFC930319
Unique NameFC930319
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length670
Libraries
Library NameType
PostHarvC1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC930319 vs. ExPASy Swiss-Prot
Match: HSDL2_BOVIN (Hydroxysteroid dehydrogenase-like protein 2 OS=Bos taurus GN=HSDL2 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.661e-11
Identity = 48/165 (29.09%), Postives = 74/165 (44.85%), Query Frame = 2
Query:   65 LKGKVALITGGGSGIGFEISTQFGKHGASVAIMGRRKQV-------LDAAVSALRSLGIKAVGFEGDVRRQEHAKKVVESTFEHFGKLDILVNAAAGNFLVSAEDLSPNGFRTVMDIDSVGTFTMCHEALKYLKKXXXXXXXXXXXXILNISATLHYTASWYQIH 538
            L G    ITG   GIG  I+ +  K GA++ I  +  Q        +  A   +++ G KA+    DVR +E     VE   E FG +DILVN A+   L +  +        +M++++ GT+      + YLKK            ILN+S  L+    W++ H
Sbjct:    8 LAGCTVFITGASRGIGKAIALKAAKDGANIVIAAKTAQAHPKLPGTIYTAAEEIKAAGGKALPCIVDVRDEEQISSAVEKAVEKFGGIDILVNNASAISLTNTLETPTKKVDLMMNVNTRGTYLTSKACIPYLKK-------SKVAHILNLSPPLNLNPLWFKQH 165          
BLAST of FC930319 vs. ExPASy Swiss-Prot
Match: BAIA1_EUBSP (Bile acid 7-dehydroxylase 1/3 OS=Eubacterium sp. (strain VPI 12708) GN=baiA1 PE=1 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 2.661e-11
Identity = 51/196 (26.02%), Postives = 91/196 (46.43%), Query Frame = 2
Query:   62 ILKGKVALITGGGSGIGFEISTQFGKHGASVAIMGRRKQVLDAAVSALRSL--GIKAVGFEGDVRRQEHAKKVVESTFEHFGKLDILVNAAAGNFLVSAEDLSPNGFRTVMDIDSVGTFTMCHEALKYLKKXXXXXXXXXXXXILNISATLHYTASWYQIHXXXXXXXXXXITRNLALEWGADYDIRVNGIAPGPI 643
            +++ K+ +ITGG  GIGF  +  F ++GA V+I G  ++ +D A++ L+ L    + +GF  D+  ++     V +  + +G+LD+++N A          +S   F+ +MDI+  G F     A + +K             I+N ++      S   I           +T  L  E     +IRV G+APG +
Sbjct:    3 LVQDKITIITGGTRGIGFAAAKLFIENGAKVSIFGETQEEVDTALAQLKELYPEEEVLGFAPDLTSRDAVMAAVGTVAQKYGRLDVMINNAGITMNSVFSRVSEEDFKNIMDINVNGVFNGAWSAYQCMK-------DAKQGVIINTASVTGIYGSLSGIGYPTSKAGVIGLTHGLGREI-IRKNIRVVGVAPGVV 190          
BLAST of FC930319 vs. ExPASy Swiss-Prot
Match: YOXD_BACSU (Uncharacterized oxidoreductase yoxD OS=Bacillus subtilis GN=yoxD PE=3 SV=2)

HSP 1 Score: 67.781 bits (164), Expect = 5.928e-11
Identity = 47/193 (24.35%), Postives = 85/193 (44.04%), Query Frame = 2
Query:   65 LKGKVALITGGGSGIGFEISTQFGKHGASVAIMGRRKQVLDAAVSALRSLGIKAVGFEGDVRRQEHAKKVVESTFEHFGKLDILVNAAAGNFLVSAEDLSPNGFRTVMDIDSVGTFTMCHEALKYLKKXXXXXXXXXXXXILNISATLHYTASWYQIHXXXXXXXXXXITRNLALEWGADYDIRVNGIAPGPI 643
            L+ K ALITGGG GIG   +    K G ++ ++GR    ++     +++LG+KA     DV+  +   + V    E  G +DIL+N A  +      DLS + +  ++ ++ +G + +    L  +              I+NIS+T     +               +T +L  E    ++IRV+ + P  +
Sbjct:    4 LQHKTALITGGGRGIGRATALALAKEGVNIGLIGRTSANVEKVAEEVKALGVKAAFAAADVKDADQVNQAVAQVKEQLGDIDILINNAGISKFGGFLDLSADEWENIIQVNLMGVYHVTRAVLPEM-------IERKAGDIINISSTAGQRGAAVTSAYSASKFAVLGLTESLMQE-VRKHNIRVSALTPSTV 188          
BLAST of FC930319 vs. ExPASy Swiss-Prot
Match: ACT3_STRCO (Putative ketoacyl reductase OS=Streptomyces coelicolor GN=actIII PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.928e-11
Identity = 55/194 (28.35%), Postives = 88/194 (45.36%), Query Frame = 2
Query:   74 KVALITGGGSGIGFEISTQFGKHGASVAIMGRRKQVLDAAVSALRSLGIKAVGFEGDVRRQEHAKKVVESTFEHFGKLDILVNAAAGNFLVSAEDLSPNGFRTVMDIDSVGTFTMCHEALKYLKKXXXXXXXXXXXXILNISATLHYTASWYQIHXXXXXXXXXXITRNLALEWGADYDIRVNGIAPGPIGDTP 655
            +VAL+TG  SGIG EI+ + GK G  V +  R ++ L   +  LR  G++A G   DVR     + +V +  E +G +D+LVN A      +  +L+   +  V++ +  G F +  + LK                I+NI++T       +              T+ L LE  A   I VN + PG + +TP
Sbjct:    7 EVALVTGATSGIGLEIARRLGKEGLRVFVCARGEEGLRTTLKELREAGVEADGRTCDVRSVPEIEALVAAVVERYGPVDVLVNNAGRPGGGATAELADELWLDVVETNLTGVFRVTKQVLK-----AGGMLERGTGRIVNIASTGGKQGVVHAAPYSASKHGVVGFTKALGLEL-ARTGITVNAVCPGFV-ETP 193          
The following BLAST results are available for this feature:
BLAST of FC930319 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 64
Match NameE-valueIdentityDescription
HSDL2_BOVIN2.661e-1129.09Hydroxysteroid dehydrogenase-like protein 2 OS=Bos... [more]
BAIA1_EUBSP2.661e-1126.02Bile acid 7-dehydroxylase 1/3 OS=Eubacterium sp. (... [more]
YOXD_BACSU5.928e-1124.35Uncharacterized oxidoreductase yoxD OS=Bacillus su... [more]
ACT3_STRCO5.928e-1128.35Putative ketoacyl reductase OS=Streptomyces coelic... [more]

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Properties
Property NameValue
Genbank descriptionC34006B10EF PostHarvC1 Citrus clementina cDNA clone C34006B10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930319 ID=FC930319; Name=FC930319; organism=Citrus clementina; type=EST; length=670bp
GCAAAAGCGGTCAGATAAAGAAATAAGCAAAATGTCGTTGGAATCTCCGT
TCAAGGCCGATATATTGAAAGGCAAAGTCGCTTTGATCACAGGAGGTGGT
TCGGGAATTGGGTTTGAGATATCAACCCAATTCGGTAAACATGGAGCTTC
AGTTGCAATCATGGGACGTCGCAAGCAAGTCCTTGATGCTGCTGTTTCTG
CTCTTCGGTCTCTTGGAATCAAGGCTGTTGGCTTTGAAGGAGATGTTCGT
AGGCAGGAACATGCGAAAAAAGTTGTGGAATCAACTTTTGAGCATTTTGG
GAAGCTTGACATTCTTGTGAATGCTGCGGCCGGCAACTTTCTTGTATCAG
CTGAGGATTTGTCCCCTAATGGGTTTCGAACAGTTATGGATATTGATTCT
GTCGGCACATTCACAATGTGCCATGAAGCACTTAAATATCTTAAAAAGGG
TGGACCTGGGAGAAGCTCAGCTGGTGGTGGATCCATTTTGAACATTAGTG
CTACTTTACATTATACAGCTTCTTGGTACCAAATACATGTAGCTGCAGCC
AAGGCAGCTGTTGATGCCATTACCAGAAACTTGGCACTGGAATGGGGAGC
TGACTATGATATTCGAGTCAATGGAATTGCCCCAGGCCCCATCGGTGATA
CCCCGGGCATGAATAAACTT
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